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KCC4 (SLC12A7) (NM_006598) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG210512
SLC12A7 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,143.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCC4
Synonyms KCC4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG210512 representing NM_006598
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGCCCACGAACTTTACGGTGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGCCGAGC
GGACGGAGGCTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGGAGATGGAAATCCAAG
AGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAGCTTCTTTGAAGGGAAGAACATGGCA
CTTTTCGAGGAGGAGATGGACAGTAACCCCATGGTGTCCTCGCTGCTCAACAAGCTGGCCAACTACACCA
ACCTGAGCCAGGGCGTGGTGGAGCACGAGGAGGACGAGGAGAGCCGGCGGCGGGAGGCCAAGGCTCCGCG
CATGGGCACCTTCATCGGCGTCTACCTGCCGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGC
CTGACGTGGATCGTGGGGGTGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACATGTA
CAATGCTGACCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGCTGGCGGGTCCTACTA
CATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGGCCTCTGCTTCTACCTGGGCACGACG
TTTGCAGGGGCCATGTATATTTTGGGGACCATCGAGATTTTTCTGACGTACATCTCCCCGGGTGCGGCCA
TCTTCCAGGCGGAGGCTGCAGGTGGCGAGGCGGCCGCCATGCTGCACAACATGCGTGTGTACGGCACGTG
CACGCTCGTGCTCATGGCCCTGGTGGTCTTCGTGGGCGTCAAGTATGTCAACAAGCTGGCGCTGGTCTTC
CTGGCCTGCGTCGTGCTGTCCATCCTGGCCATCTATGCCGGCGTCATCAAGTCTGCCTTCGACCCCCCGG
ACATCCCGGTCTGCCTCCTGGGGAACCGCACGCTGTCACGGCGCAGCTTCGATGCCTGCGTCAAGGCCTA
CGGCATCCACAACAACTCAGCCACCTCCGCGCTCTGGGGCCTCTTCTGCAACGGCTCCCAGCCCAGCGCC
GCCTGTGACGAGTACTTCATCCAGAACAACGTCACCGAAATCCAGGGCATCCCGGGCGCGGCCAGTGGTG
TCTTCCTGGAGAACCTGTGGAGTACGTACGCGCACGCGGGGGCGTTTGTGGAGAAGAAAGGTGTGCCCTC
GGTGCCCGTGGCAGAGGAGAGCCGTGCCAGCACACTGCCCTACGTGCTCACCGACATCGCGGCCTCCTTC
ACCCTGCTGGTTGGCATCTACTTCCCTTCCGTGACCGGTATCATGGCGGGTTCAAACCGGTCCGGGGACC
TCAAGGATGCACAGAAGTCCATCCCCACGGGGACCATCCTGGCCATAGTGACGACGTCTTTCATCTATCT
CTCCTGCATTGTGCTGTTTGGGGCCTGCATTGAAGGCGTGGTCTTACGAGATAAGTTCGGGGAGGCCCTG
CAGGGGAACCTGGTCATCGGCATGCTGGCCTGGCCCTCCCCCTGGGTCATCGTCATCGGCTCCTTCTTCT
CCACCTGCGGTGCCGGCCTGCAGACCCTCACGGGGGCACCGCGCCTACTGCAGGCCATTGCCCGTGACGG
CATCGTCCCCTTCCTGCAGGTGTTTGGCCACGGGAAGGCCAACGGGGAGCCCACGTGGGCGCTGCTGCTG
ACAGTCCTCATCTGCGAGACTGGCATCCTCATCGCCTCTCTGGACAGCGTGGCCCCGATCCTCTCCATGT
TCTTCCTCATGTGCTACCTGTTCGTGAACCTGGCCTGCGCCGTGCAGACCCTGCTACGTACCCCCAACTG
GCGTCCACGCTTCAAGTTCTACCACTGGACCCTGTCCTTTCTGGGTATGAGCCTGTGCCTGGCGCTGATG
TTCATCTGCTCCTGGTACTACGCGCTGTCCGCCATGCTCATCGCTGGCTGCATCTACAAGTACATCGAGT
ACCGCGGGGCCGAGAAGGAGTGGGGCGATGGCATCCGTGGCCTATCCCTGAACGCCGCCCGCTACGCCCT
GCTGCGCGTGGAGCACGGTCCCCCCCACACCAAGAACTGGAGGCCCCAGGTGCTGGTGATGCTGAACCTG
GACGCGGAGCAGGCCGTGAAGCACCCCCGCCTGCTGTCCTTCACGTCGCAGCTGAAGGCCGGCAAGGGCC
TGACCATCGTGGGCTCGGTGCTGGAGGGGACGTACCTGGACAAGCACATGGAGGCTCAGCGGGCCGAGGA
GAACATACGGTCCCTAATGAGCACAGAGAAGACCAAGGGCTTCTGCCAGCTGGTGGTCTCGTCCAGCCTG
CGGGATGGCATGTCCCACCTGATCCAGTCGGCCGGCCTGGGCGGCCTGAAGCACAACACGGTGCTCATGG
CCTGGCCCGCATCCTGGAAGCAGGAGGACAACCCCTTCTCCTGGAAGAACTTTGTAGACACCGTCCGCGA
CACCACCGCCGCGCACCAGGCTCTGCTGGTGGCCAAGAACGTCGACTCGTTTCCGCAAAACCAGGAGCGC
TTCGGCGGGGGCCACATCGACGTGTGGTGGATCGTGCACGACGGCGGCATGCTCATGCTGCTGCCCTTCC
TGCTGCGCCAGCACAAGGTGTGGAGGAAGTGCCGGATGCGTATCTTCACCGTGGCCCAGGTGGACGACAA
CAGCATCCAGATGAAGAAGGACCTGCAGATGTTCTTGTATCACTTGCGCATCAGCGCCGAGGTGGAGGTG
GTGGAGATGGTTGAAAACGACATATCTGCTTTCACCTACGAGAGGACACTAATGATGGAGCAGAGGTCGC
AGATGCTGAAGCAGATGCAGCTGTCCAAGAACGAGCAGGAGCGAGAGGCCCAGCTGATCCACGACAGGAA
CACCGCGTCCCACACCGCGGCGGCAGCCAGGACCCAAGCGCCGCCTACGCCAGACAAGGTGCAGATGACC
TGGACCAGGGAGAAGCTGATCGCTGAGAAGTACAGGAGCAGAGACACCAGCCTATCCGGTTTCAAAGACC
TCTTCAGCATGAAGCCGGACCAGTCCAACGTCAGGCGGATGCACACGGCTGTGAAGCTCAATGGCGTCGT
CCTCAACAAGTCCCAGGATGCGCAGCTGGTCCTGCTCAACATGCCAGGTCCTCCCAAAAACCGGCAGGGA
GACGAGAACTACATGGAGTTTCTTGAAGTCCTGACCGAGGGGCTGAACAGAGTCCTCCTGGTCAGGGGTG
GCGGCCGGGAGGTGATCACCATCTACTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG210512 representing NM_006598
Red=Cloning site Green=Tags(s)

MPTNFTVVPVEAHADGGGDETAERTEAPGTPEGPEPERPSPGDGNPRENSPFLNNVEVEQESFFEGKNMA
LFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEESRRREAKAPRMGTFIGVYLPCLQNILGVILFLR
LTWIVGVAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTT
FAGAMYILGTIEIFLTYISPGAAIFQAEAAGGEAAAMLHNMRVYGTCTLVLMALVVFVGVKYVNKLALVF
LACVVLSILAIYAGVIKSAFDPPDIPVCLLGNRTLSRRSFDACVKAYGIHNNSATSALWGLFCNGSQPSA
ACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAHAGAFVEKKGVPSVPVAEESRASTLPYVLTDIAASF
TLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEAL
QGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQTLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLL
TVLICETGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALM
FICSWYYALSAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNL
DAEQAVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHMEAQRAEENIRSLMSTEKTKGFCQLVVSSSL
RDGMSHLIQSAGLGGLKHNTVLMAWPASWKQEDNPFSWKNFVDTVRDTTAAHQALLVAKNVDSFPQNQER
FGGGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEV
VEMVENDISAFTYERTLMMEQRSQMLKQMQLSKNEQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMT
WTREKLIAEKYRSRDTSLSGFKDLFSMKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKNRQG
DENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006598
ORF Size 3249 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006598.1, NP_006589.1
RefSeq Size 5239 bp
RefSeq ORF 3252 bp
Locus ID 10723
UniProt ID Q9Y666
Cytogenetics 5p15.33
Protein Families Transmembrane
Summary Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_006598" in other vectors (6)
SKU Description Size Price
RC210512 SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) 10 ug
$943.00
RC210512L1 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged 10 ug
$1,243.00
RC210512L2 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged 10 ug
$1,243.00
RC210512L3 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged 10 ug
$1,243.00
RC210512L4 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged 10 ug
$1,243.00
SC303797 SLC12A7 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) 10 ug
$944.00

Plasmid Map for RG210512

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG210512
Circular map for RG210512

file-alt Citations

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