KCC4 (SLC12A7) (NM_006598) Human Tagged ORF Clone
SKU
RC210512
SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7)
$943.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | KCC4 |
| Synonyms | KCC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
AscI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006598 |
| ORF Size | 3249 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006598.3 |
| RefSeq Size | 5239 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 10723 |
| UniProt ID | Q9Y666 |
| Cytogenetics | 5p15.33 |
| Protein Families | Transmembrane |
| MW | 118.9 kDa |
| Summary | Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006598" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210512L1 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged | 10 ug |
$1,243.00
|
|
| RC210512L2 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged | 10 ug |
$1,243.00
|
|
| RC210512L3 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged | 10 ug |
$1,243.00
|
|
| RC210512L4 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged | 10 ug |
$1,243.00
|
|
| RG210512 | SLC12A7 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) | 10 ug |
$1,143.00
|
|
| SC303797 | SLC12A7 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) | 10 ug |
$944.00
|
|
Citations
SLC12A7 alters adrenocortical carcinoma cell adhesion properties to promote an aggressive invasive behavior
Brown, Murtha, Rubinstein et al
Cell Commun. Signal (2018) 16 (1), 27 DOI: 10.1186/s12964-018-0243-0
Brown, Murtha, Rubinstein et al
Cell Commun. Signal (2018) 16 (1), 27 DOI: 10.1186/s12964-018-0243-0
AI Summary: OriGene’s Myc-DDK tagged pCMV6-Entry and pCMV6-Entry/SLC12A7-ORF plasmid expression vectors were used to enforce SLC12A7 overexpression in SW-13 adrenocortical carcinoma cells, enabling the study of its effects on cell adhesion and invasive behavior.
Snippet: Myc-DDK tagged pCMV6-Entry and pCMV6-Entry/SLC12A7-ORF plasmid expression vectors (Origene) were transfected into SW-13 cells using Lipofectamine 3000 (ThermoFisher) according to the manufacturer's recommendations in 6-well plates with cells grown Cached
Showing 1-1 of 1 papers.
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