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KCC4 (SLC12A7) (NM_006598) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC210512L4
Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,243.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCC4
Synonyms KCC4
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC210512).
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006598
ORF Size 3249 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006598.1
RefSeq Size 5239 bp
RefSeq ORF 3252 bp
Locus ID 10723
UniProt ID Q9Y666
Cytogenetics 5p15.33
Protein Families Transmembrane
MW 118.9 kDa
Summary Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).UniProtKB/Swiss-Prot Function
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"NM_006598" in other vectors (6)
SKU Description Size Price
RC210512 SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) 10 ug
$943.00
RC210512L1 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged 10 ug
$1,243.00
RC210512L2 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), mGFP tagged 10 ug
$1,243.00
RC210512L3 Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7), Myc-DDK-tagged 10 ug
$1,243.00
RG210512 SLC12A7 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) 10 ug
$1,143.00
SC303797 SLC12A7 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7) 10 ug
$944.00

Plasmid Map for RC210512L4

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file-download TXT (FASTA format)

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Cloning scheme for RC210512L4
Circular map for RC210512L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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