RAD51C (NM_058216) Human Tagged ORF Clone

  • TrueORF®
SKU
RG210462
RAD51C (tGFP-tagged) - Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1
  $886.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RAD51C
Synonyms BROVCA3; FANCO; R51H3; RAD51L2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG210462 representing NM_058216
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGTTTCCCGCTGTCTCCAGCGGTGC
GGGTGAAGCTGGTGTCTGCGGGGTTCCAGACTGCTGAGGAACTCCTAGAGGTGAAACCCTCCGAGCTTAG
CAAAGAAGTTGGGATATCTAAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCACA
AATAAACCAAGATATGCTGGTACATCTGAGTCACACAAGAAGTGTACAGCACTGGAACTTCTTGAGCAGG
AGCATACCCAGGGCTTCATAATCACCTTCTGTTCAGCACTAGATGATATTCTTGGGGGTGGAGTGCCCTT
AATGAAAACAACAGAAATTTGTGGTGCACCAGGTGTTGGAAAAACACAATTATGTATGCAGTTGGCAGTA
GATGTGCAGATACCAGAATGTTTTGGAGGAGTGGCAGGTGAAGCAGTTTTTATTGATACAGAGGGAAGTT
TTATGGTTGATAGAGTGGTAGACCTTGCTACTGCCTGCATTCAGCACCTTCAGCTTATAGCAGAAAAACA
CAAGGGAGAGGAACACCGAAAAGCTTTGGAGGATTTCACTCTTGATAATATTCTTTCTCATATTTATTAT
TTTCGCTGTCGTGACTACACAGAGTTACTGGCACAAGTTTATCTTCTTCCAGATTTCCTTTCAGAACACT
CAAAGGTTCGACTAGTGATAGTGGATGGTATTGCTTTTCCATTTCGTCATGACCTAGATGACCTGTCTCT
TCGTACTCGGTTATTAAATGGCCTAGCCCAGCAAATGATCAGCCTTGCAAATAATCACAGATTAGCTGTA
ATTTTAACCAATCAGATGACAACAAAGATTGATAGAAATCAGGCCTTGCTTGTTCCTGCATTAGGGGAAA
GTTGGGGACATGCTGCTACAATACGGCTAATCTTTCATTGGGACCGAAAGCAAAGGTTGGCAACATTGTA
CAAGTCACCCAGCCAGAAGGAATGCACAGTACTGTTTCAAATCAAACCTCAGGGATTTAGAGATACTGTT
GTTACTTCTGCATGTTCATTGCAAACAGAAGGTTCCTTGAGCACCCGGAAACGGTCACGAGACCCAGAGG
AAGAATTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG210462 representing NM_058216
Red=Cloning site Green=Tags(s)

MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLT
NKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAV
DVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYY
FRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAV
ILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTV
VTSACSLQTEGSLSTRKRSRDPEEEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_058216
ORF Size 1128 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_058216.3
RefSeq Size 1295 bp
RefSeq ORF 1131 bp
Locus ID 5889
UniProt ID O43502
Cytogenetics 17q22
Protein Families Druggable Genome
Protein Pathways Homologous recombination
Summary This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_058216" in other vectors (7)
SKU Description Size Price
RC210462 RAD51C (Myc-DDK-tagged)-Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1 10 ug
$686.00
RC210462L1 Lenti ORF clone of Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC210462L2 Lenti ORF clone of Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1, mGFP tagged 10 ug
$986.00
RC210462L3 Lenti ORF clone of Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC210462L4 Lenti ORF clone of Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1, mGFP tagged 10 ug
$986.00
SC109703 RAD51C (untagged)-Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1 10 ug
$457.00
SC321013 RAD51C (untagged)-Human RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 1 10 ug
$457.00

Plasmid Map for RG210462

Download sequence for

Cloning scheme for RG210462

Circular map for RG210462

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