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GRPR (NM_005314) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG210362
GRPR (tGFP-tagged) - Human gastrin-releasing peptide receptor (GRPR)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRPR
Synonyms BB2; BB2R; BRS2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG210362 representing NM_005314
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTAAATGACTGTTTCCTTCTGAACTTGGAGGTGGACCATTTCATGCACTGCAACATCTCCAGTC
ACAGTGCGGATCTCCCCGTGAACGATGACTGGTCCCACCCGGGGATCCTCTATGTCATCCCTGCAGTTTA
TGGGGTTATCATTCTGATAGGCCTCATTGGCAACATCACTTTGATCAAGATCTTCTGTACAGTCAAGTCC
ATGCGAAACGTTCCAAACCTGTTCATTTCCAGTCTGGCTTTGGGAGACCTGCTCCTCCTAATAACGTGTG
CTCCAGTGGATGCCAGCAGGTACCTGGCTGACAGATGGCTATTTGGCAGGATTGGCTGCAAACTGATCCC
CTTTATACAGCTTACCTCTGTTGGGGTGTCTGTCTTCACACTCACGGCGCTCTCGGCAGACAGATACAAA
GCCATTGTCCGGCCAATGGATATCCAGGCCTCCCATGCCCTGATGAAGATCTGCCTCAAAGCCGCCTTTA
TCTGGATCATCTCCATGCTGCTGGCCATTCCAGAGGCCGTGTTTTCTGACCTCCATCCCTTCCATGAGGA
AAGCACCAACCAGACCTTCATTAGCTGTGCCCCATACCCACACTCTAATGAGCTTCACCCCAAAATCCAT
TCTATGGCTTCCTTTCTGGTCTTCTACGTCATCCCACTGTCGATCATCTCTGTTTACTACTACTTCATTG
CTAAAAATCTGATCCAGAGTGCTTACAATCTTCCCGTGGAAGGGAATATACATGTCAAGAAGCAGATTGA
ATCCCGGAAGCGACTTGCCAAGACAGTGCTGGTGTTTGTGGGCCTGTTCGCCTTCTGCTGGCTCCCCAAT
CATGTCATCTACCTGTACCGCTCCTACCACTACTCTGAGGTGGACACCTCCATGCTCCACTTTGTCACCA
GCATCTGTGCCCGCCTCCTGGCCTTCACCAACTCCTGCGTGAACCCCTTTGCCCTCTACCTGCTGAGCAA
GAGTTTCAGGAAACAGTTCAACACTCAGCTGCTCTGTTGCCAGCCTGGCCTGATCATCCGGTCTCACAGC
ACTGGAAGGAGTACAACCTGCATGACCTCCCTCAAGAGTACCAACCCCTCCGTGGCCACCTTTAGCCTCA
TCAATGGAAACATCTGTCACGAGCGGTATGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG210362 representing NM_005314
Red=Cloning site Green=Tags(s)

MALNDCFLLNLEVDHFMHCNISSHSADLPVNDDWSHPGILYVIPAVYGVIILIGLIGNITLIKIFCTVKS
MRNVPNLFISSLALGDLLLLITCAPVDASRYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYK
AIVRPMDIQASHALMKICLKAAFIWIISMLLAIPEAVFSDLHPFHEESTNQTFISCAPYPHSNELHPKIH
SMASFLVFYVIPLSIISVYYYFIAKNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPN
HVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLIIRSHS
TGRSTTCMTSLKSTNPSVATFSLINGNICHERYV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005314
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005314.1
RefSeq Size 2681 bp
RefSeq ORF 1155 bp
Locus ID 2925
UniProt ID P30550
Cytogenetics Xp22.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
Summary Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. provided by RefSeq, Jul 2008
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"NM_005314" in other vectors (6)
SKU Description Size Price
RC210362 GRPR (Myc-DDK-tagged)-Human gastrin-releasing peptide receptor (GRPR) 10 ug
$457.00
RC210362L1 Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), Myc-DDK-tagged 10 ug
$757.00
RC210362L2 Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), mGFP tagged 10 ug
$757.00
RC210362L3 Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), Myc-DDK-tagged 10 ug
$757.00
RC210362L4 Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), mGFP tagged 10 ug
$757.00
SC116819 GRPR (untagged)-Human gastrin-releasing peptide receptor (GRPR) 10 ug
$457.00

Plasmid Map for RG210362

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG210362
Circular map for RG210362

file-alt Citations

A Selective Carborane-Functionalized Gastrin-Releasing Peptide Receptor Agonist as Boron Delivery Agent for Boron Neutron Capture Therapy
Hoppenz, Els-Heindl, Kellert et al
J. Org. Chem. (2019)
Cited by 54
AI Summary: In this study, OriGene’s pCMV6_NMBR-tGFP, pCMV6_GRPR-tGFP, and pCMV6_BRS-3-tGFP plasmids were used to express specific G protein-coupled receptors in cells, enabling the evaluation of the selectivity and uptake of the novel boron delivery peptide conjugates for boron neutron capture therapy.
Snippet: …Hoechst33342 and D-Galactose was purchased from Sigma-Aldrich. pCMV6_NMBR-tGFP, pCMV6_GRPR-tGFP, pCMV6_BRS-3-tGFP were supplied from OriGene Technologies, Inc. …
Showing 1-1 of 1 papers.
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