EZH2 (NM_004456) Human Tagged ORF Clone
- TrueORF®
SKU
RG202054
EZH2 (tGFP-tagged) - Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1
$789.00
$1,231.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EZH2 |
| Synonyms | ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004456 |
| ORF Size | 2253 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004456.5 |
| RefSeq Size | 2695 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 2146 |
| UniProt ID | Q15910 |
| Cytogenetics | 7q36.1 |
| Domains | myb_DNA-binding, SET |
| Protein Families | Druggable Genome, Transcription Factors |
| Summary | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Feb 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004456" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202054 | EZH2 (Myc-DDK-tagged)-Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 | 10 ug |
$1,031.00
|
|
| RC202054L1 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,331.00
|
|
| RC202054L2 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mGFP tagged | 10 ug |
$1,331.00
|
|
| RC202054L3 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,331.00
|
|
| RC202054L4 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mGFP tagged | 10 ug |
$1,331.00
|
|
| SC128144 | EZH2 (untagged)-Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 | 10 ug |
$1,033.00
|
|
Citations
EZH2 regulates expression of FOXC1 by mediating H3K27me3 in breast cancers
Zheng, Li, Yi et al
Acta Pharmacol Sin (2020)
Zheng, Li, Yi et al
Acta Pharmacol Sin (2020)
Cited by 21
Biophysical characterization of histone H3.3 K27M point mutation
Hetey, Boros-Oláh, Kuik-Rózsa et al
Biochem Biophys Res Commun (2017) 490 (3), 868-875 DOI: 10.1016/j.bbrc.2017.06.133
Hetey, Boros-Oláh, Kuik-Rózsa et al
Biochem Biophys Res Commun (2017) 490 (3), 868-875 DOI: 10.1016/j.bbrc.2017.06.133
Cited by 17
AI Summary: In this study, OriGene products—including the H3F3A (NM_002107) cDNA clone—were used to express wild-type and K27M mutant histone H3.3 proteins, enabling the biophysical and cellular characterization of the mutation’s effects on nucleosome stability and Ezh2 dynamics.
Showing 1-2 of 2 papers.
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