EZH2 (NM_004456) Human Untagged Clone
SKU
SC128144
EZH2 (untagged)-Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1
$1,033.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | EZH2 |
| Synonyms | ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_004456 |
| Insert Size | 2256 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004456.3, NP_004447.2 |
| RefSeq Size | 2695 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 2146 |
| UniProt ID | Q15910 |
| Cytogenetics | 7q36.1 |
| Domains | myb_DNA-binding, SET |
| Protein Families | Druggable Genome, Transcription Factors |
| Summary | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Feb 2011 Transcript Variant: This variant (1) encodes the longest isoform (a). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004456" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202054 | EZH2 (Myc-DDK-tagged)-Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 | 10 ug |
$1,031.00
|
|
| RC202054L1 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,331.00
|
|
| RC202054L2 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mGFP tagged | 10 ug |
$1,331.00
|
|
| RC202054L3 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,331.00
|
|
| RC202054L4 | Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mGFP tagged | 10 ug |
$1,331.00
|
|
| RG202054 | EZH2 (tGFP-tagged) - Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 | 10 ug |
$789.00
$1,231.00
|
|
Citations
EZH2 modulates the DNA methylome and controls T cell adhesion through junctional adhesion molecule-A in lupus patients
Tsou, Coit, Kilian et al
Arthritis & rheumatology (Hoboken, N.J.) (2017)
Tsou, Coit, Kilian et al
Arthritis & rheumatology (Hoboken, N.J.) (2017)
AI Summary: OriGene’s EZH2 expression plasmid (EZH2; control vector pCMV6-XL5) was used to overexpress EZH2 in naïve CD4+ T cells, enabling the study of EZH2’s role in DNA methylation and T cell adhesion in lupus pathogenesis.
Snippet: After isolation and purification, naīve CD4+ T cells from healthy subjects were transfected with 0.1 μg of EZH2 (Origene;control vector pCMV6-XL5) and cultured in RPMI media supplemented with 10% fetal bovine serum (FBS) and 2mM L-glutamine. After 5 Page 5. ...;... After isolation and purification, naīve CD4+ T cells from healthy subjects were transfected with 0.1 μg of EZH2 (Origene;control vector pCMV6-XL5) and cultured in RPMI media supplemented with 10% fetal bovine serum (FBS) and 2mM L-glutamine. After 5 Page 5.
TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein
Bhatnagar, Gazin, Chamberlain et al
Nature (2014)
Bhatnagar, Gazin, Chamberlain et al
Nature (2014)
AI Summary: OriGene’s RNF2 protein was used in in vitro ubiquitination assays to investigate the ubiquitin ligase activity of TRIM37 in the study.
Snippet: In vitro ubiquitination reactions were set up containing purified TRIM37 or TRIM37(C18R) (500 ng to 2 µg), a combination of BMI1 (500 ng;Abcam) and RNF2 (500 ng;Origene) mixed together, or BRCA1 (500 ng;Abcam) plus H2A or H2B (5X;Boston Biochemicals), 10 nM UBE1 (E1;Boston Biochemicals), 10 nM UBCH5B (E2;Boston Biochemicals), and 10 nM HA-ubiquitin aldehyde (Boston Biochemicals) in 10 µl of reaction buffer (50 mM Tris-Cl, pH 7.9, 5 mM MgCl2, 2 mM NaF, 100 mM dithiothreitol (DTT) and 2 mM ATP)
Down-regulation of the miR-25 and miR-30d contributes to the development of anaplastic thyroid carcinoma targeting the polycomb protein EZH2
Esposito, Tornincasa, Pallante et al
J. Clin. Endocrinol. Metab. (2012) 97 (5), E710-8 DOI: 10.1210/jc.2011-3068
Esposito, Tornincasa, Pallante et al
J. Clin. Endocrinol. Metab. (2012) 97 (5), E710-8 DOI: 10.1210/jc.2011-3068
Cited by 117
AI Summary: In this study, the pCMV6-EZH2 expression vector from OriGene was used to investigate the role of EZH2 in anaplastic thyroid carcinoma cells following manipulation of miR-25 and miR-30d levels.
Snippet: control vector) were purchased at Cell Biolabs (San Diego, CA). The pCMV6-EZH2 vector was purchased at Origene Technologies (Rockville, MD). For transfection of miRNA oligonucleotides, cells were transfected with 50
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
