PPAR gamma (PPARG) (NM_138712) Human Tagged ORF Clone
- TrueORF®
SKU
RG201538
PPARG (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1
$886.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PPAR gamma |
| Synonyms | CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_138712 |
| ORF Size | 1431 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_138712.4 |
| RefSeq Size | 1892 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 5468 |
| UniProt ID | P37231 |
| Cytogenetics | 3p25.2 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Protein Pathways | Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer |
| Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_138712" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201538 | PPARG (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$686.00
|
|
| RC201538L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201538L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| SC124177 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$686.00
|
|
| SC317792 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$732.00
|
|
| SC322218 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$686.00
|
|
Citations
Identification of Crocetin as a Dual Agonist of GPR40 and GPR120 Responsible for the Antidiabetic Effect of Saffron
Zhao, Ahn, Nam et al
Nutrients (2023) 15 (22) DOI: 10.3390/nu15224774
Zhao, Ahn, Nam et al
Nutrients (2023) 15 (22) DOI: 10.3390/nu15224774
Cited by 14
AI Summary: OriGene’s pCMV6-hPPARG-GFP plasmid (RG201538) was used in this study to enable the analysis of PPARγ activation, helping demonstrate that crocetin selectively activates GPR40 and GPR120 rather than PPARγ.
Snippet: ell in DMEM containing 10% FBS and 1% 100× antibiotic–antimycotic solution. After a 24 h incubation, the cells were cotransfected with pPPRE-TK-Luc (addgene, #1015) and pCMV6-hPPARG-GFP (RG201538, OriGene Technologies, Inc., Rockville, MD, US) using Lipofetamine 3000 (Cat. # L3000015, Invitrogen, Waltham, MA, US). Following a 3 h transfection, the cells were treated with the compounds of intere
Ethyl Gallate Dual-Targeting PTPN6 and PPARγ Shows Anti-Diabetic and Anti-Obese Effects
Ahn, Kim, Nam et al
Int J Mol Sci (2022) 23 (9) DOI: 10.3390/ijms23095020
Ahn, Kim, Nam et al
Int J Mol Sci (2022) 23 (9) DOI: 10.3390/ijms23095020
Cited by 29
AI Summary: OriGene’s pCMV6-hPPARG-GFP plasmid (RG201538) was used in this study to enable the expression of human PPARγ for functional assays assessing the antagonist activity of ethyl gallate.
Snippet: ipped FBS and 1% penicillin at a density of 1.5 × 104 cells/well. After incubation for 24 h, the cells were co-transfected with pPPRE-TK-luc (Addgene #1015, Watertown, MA, USA) and pCMV6-hPPARG-GFP (Origene (Rockville, MD, USA), RG201538) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. After 6 h of transfection, cells were treated and incub
Structure-Activity Relationship of Synthetic Ginkgolic Acid Analogs for Treating Type 2 Diabetes by PTPN9 Inhibition
Kim, Son, Ahn et al
Int J Mol Sci (2022) 23 (7) DOI: 10.3390/ijms23073927
Kim, Son, Ahn et al
Int J Mol Sci (2022) 23 (7) DOI: 10.3390/ijms23073927
Cited by 8
AI Summary: OriGene products pCMV6-hFFAR4-GFP (RG201538) and FFAR4 (RG231184) plasmids were used in this study to co-transfect cells for functional assays assessing the effects of synthetic ginkgolic acid analogs on glucose uptake and insulin signaling pathways.
Snippet: ty of 1.5 × 104 cells/well. After 24 h of incubation, the cells were co-transfected using the Cignal PPAR Reporter (QIAGEN, Cignal PPAR (Luc) Reporter kit, CCS-3026L) and pCMV6-hFFAR4-GFP (RG201538, OriGene Technologies, Inc., Rockville, MD, USA) or with SRE plasmids (pGL4.33 (luc2P/SRE/Hygro), Promega, Madison, WI, USA) and FFAR4 (RG231184, OriGene Technologies, Inc., Rockville, MD, USA) using a
Structure-Activity Relationship Study and Biological Evaluation of 2-(Disubstituted phenyl)-indole-5-propanoic Acid Derivatives as GPR40 Full Agonists
Zhao, Yoon, Yoo et al
J Med Chem (2021) 64 (7), 4130-4149 DOI: 10.1021/acs.jmedchem.1c00031
Zhao, Yoon, Yoo et al
J Med Chem (2021) 64 (7), 4130-4149 DOI: 10.1021/acs.jmedchem.1c00031
Cited by 11
AI Summary: OriGene's pCMV6-hPPARG-GFP plasmid (RG201538) was used for co-transfection experiments to study the activity of PPARγ in the evaluation of GPR40 agonist compounds.
Snippet: G-protein-coupled receptor 40 (GPR40) is considered as an attractive drug target for treating type 2 diabetes, owing to its role in the free fatty acid-mediated increase in glucose-stimulated insul...;the cells were co-transfected using the Cignal PPARReporter (QIAGEN, Cignal PPAR (Luc) Reporter kit, CCS-3026L)along with pCMV6-hPPARG-GFP (Origene, RG201538) usingLipofetamine2000 (Invitrogen) according to the manufacturer’sinstructions
Showing 1-4 of 4 papers.
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