PPAR gamma (PPARG) (NM_138712) Human Untagged Clone
SKU
SC322218
PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PPAR gamma |
| Synonyms | CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_138712 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_138712.2, NP_619726.1 |
| RefSeq Size | 1883 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 5468 |
| UniProt ID | P37231 |
| Cytogenetics | 3p25.2 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Protein Pathways | Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer |
| Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) differs in the 5' UTR and 5' coding region compared to variant 2. Variants 1, 3, 4, 6, and 7 all encode the same isoform (1), which has a shorter, distinct N-terminus compared to isoform 2. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_138712" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201538 | PPARG (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$686.00
|
|
| RC201538L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC201538L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG201538 | PPARG (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$886.00
|
|
| SC124177 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$686.00
|
|
| SC317792 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 | 10 ug |
$732.00
|
|
Citations
Peroxisome proliferator-activated receptor γ-mediated induction of microRNA-145 opposes tumor phenotype in colorectal cancer
Panza, Votino, Gentile et al
Biochim. Biophys. Acta (2014)
Panza, Votino, Gentile et al
Biochim. Biophys. Acta (2014)
Cited by 28
AI Summary: OriGene’s pCMV6-XL4 plasmid vector containing the PPARγ coding region was used in this study to ectopically express PPARγ in colorectal cancer cell lines, enabling the investigation of its regulatory effect on miR-145 expression and tumor phenotype.
Snippet: described (9). 2.8. Expression vectors and mutagenesis. The pCMV6-XL4 plasmid vector (OriGene, Rockville, MD) containing the PPARγ coding region was used for Cignal PPAR (luc) reporter assay (QIAGEN). The luciferase ... Cite SaveSaving
CD146(+) Human Umbilical Cord Perivascular Cells Maintain Stemness under Hypoxia and as a Cell Source for Skeletal Regeneration
Tsang, Shu, Kwok et al
PLoS ONE (2013) 8 (10), e76153 DOI: 10.1371/journal.pone.0076153
Tsang, Shu, Kwok et al
PLoS ONE (2013) 8 (10), e76153 DOI: 10.1371/journal.pone.0076153
Cited by 70
AI Summary: OriGene’s PPAR-γ expression vector was used in this study to investigate the regulatory role of PPAR-γ on HIF-2α promoter activity in human umbilical cord perivascular cells (HUCPVCs) under hypoxic conditions.
Snippet: directed mutagenesis Kit (Promega). HUCPVCs were seeded in 24-well plates for 24 h, 900 ng PPAR-c-expression vector (Origene) was co-transfected with 300 ng wild-type or mutated Hif-2a-pGL3 constructs for 24h. 0.05 mg pRL ... Cite SaveSaving
CD146+ Human Umbilical Cord Perivascular Cells Maintain Stemness under Hypoxia and as a Cell Source for Skeletal Regeneration
Tsang, Shu, Kwok et al
PLOS ONE (2013) 8 (10), e76153
Tsang, Shu, Kwok et al
PLOS ONE (2013) 8 (10), e76153
AI Summary: OriGene’s PPAR-γ expression vector was used for co-transfection experiments to study gene regulation in CD146+ human umbilical cord perivascular cells under hypoxic conditions in this paper.
Snippet: HUCPVCs were seeded in 24-well plates for 24 h, 900 ng PPAR-γ-expression vector (Origene) was co-transfected with 300 ng wild-type or mutated Hif-2α-pGL3 constructs for 24 h. 0.05 µg pRL-CMV plasmid expressing Renilla luciferase was co-transfected in each
Showing 1-3 of 3 papers.
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