ESE1 (ELF3) (NM_004433) Human Tagged ORF Clone

SKU RG200631

ELF3 (tGFP-tagged) - Human E74-like factor 3 (ets domain transcription factor, epithelial-specific ) (ELF3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ESE1
• Synonyms: EPR-1; ERT; ESE-1; ESX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200631 representing NM_004433; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCAACCTGTGAGATTAGCAACATTTTTAGCAACTACTTCAGTGCGATGTACAGCTCGGAGGACT; CCACCCTGGCCTCTGTTCCCCCTGCTGCCACCTTTGGGGCCGATGACTTGGTACTGACCCTGAGCAACCC; CCAGATGTCATTGGAGGGTACAGAGAAGGCCAGCTGGTTGGGGGAACAGCCCCAGTTCTGGTCGAAGACG; CAGGTTCTGGACTGGATCAGCTACCAAGTGGAGAAGAACAAGTACGACGCAAGCGCCATTGACTTCTCAC; GATGTGACATGGATGGCGCCACCCTCTGCAATTGTGCCCTTGAGGAGCTGCGTCTGGTCTTTGGGCCTCT; GGGGGACCAACTCCATGCCCAGCTGCGAGACCTCACTTCCAGCTCTTCTGATGAGCTCAGTTGGATCATT; GAGCTGCTGGAGAAGGATGGCATGGCCTTCCAGGAGGCCCTAGACCCAGGGCCCTTTGACCAGGGCAGCC; CCTTTGCCCAGGAGCTGCTGGACGACGGTCAGCAAGCCAGCCCCTACCACCCCGGCAGCTGTGGCGCAGG; AGCCCCCTCCCCTGGCAGCTCTGACGTCTCCACCGCAGGGACTGGTGCTTCTCGGAGCTCCCACTCCTCA; GACTCCGGTGGAAGTGACGTGGACCTGGATCCCACTGATGGCAAGCTCTTCCCCAGCGATGGTTTTCGTG; ACTGCAAGAAGGGGGATCCCAAGCACGGGAAGCGGAAACGAGGCCGGCCCCGAAAGCTGAGCAAAGAGTA; CTGGGACTGTCTCGAGGGCAAGAAGAGCAAGCACGCGCCCAGAGGCACCCACCTGTGGGAGTTCATCCGG; GACATCCTCATCCACCCGGAGCTCAACGAGGGCCTCATGAAGTGGGAGAATCGGCATGAAGGCGTCTTCA; AGTTCCTGCGCTCCGAGGCTGTGGCCCAACTATGGGGCCAAAAGAAAAAGAACAGCAACATGACCTACGA; GAAGCTGAGCCGGGCCATGAGGTACTACTACAAACGGGAGATCCTGGAACGGGTGGATGGCCGGCGACTC; GTCTACAAGTTTGGCAAAAACTCAAGCGGCTGGAAGGAGGAAGAGGTTCTCCAGAGTCGGAAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200631 representing NM_004433; Red=Cloning site Green=Tags(s); ; MAATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKT; QVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWII; ELLEKDGMAFQEALDPGPFDQGSPFAQELLDDGQQASPYHPGSCGAGAPSPGSSDVSTAGTGASRSSHSS; DSGGSDVDLDPTDGKLFPSDGFRDCKKGDPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIR; DILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRL; VYKFGKNSSGWKEEEVLQSRN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004433
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004433.5
• RefSeq Size: 5036 bp
• RefSeq ORF: 1116 bp
• Locus ID: 1999
• UniProt ID: P78545
• Cytogenetics: 1q32.1
• Domains: AT_hook, ETS, SAM_PNT
• Protein Families: Transcription Factors
• Summary: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).[UniProtKB/Swiss-Prot Function]