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LTK (NM_002344) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC600053
LTK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens leukocyte receptor tyrosine kinase, transcript variant 1, Signal peptide (1-16) plus EC domain (17-424)
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LTK
Synonyms TYK1
Vector pCMV6-XL5-DDK-His
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
>RC600053 representing leader sequence plus the extracellular domain region of NM_002344
Red=Cloning site Blue=ORF Green=Tags(s)

GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
CGATCGCC

ATGGGCTGCTGGGGACAGCTGCTGGTGTGGTTCGGAGCCGCGGGCGCCATTCTCTGCTCTAGCCCGGGGT
CCCAGGAGACTTTTCTGCGGTCCTCGCCCCTGCCGCTGGCAAGTCCCAGCCCCCGGGACCCGAAAGTCAG
CGCCCCGCCTAGTATCTTGGAGCCAGCCTCCCCGCTGAATTCTCCGGGCACCGAGGGGTCTTGGCTGTTT
TCTACCTGCGGGGCCAGCGGCCGGCATGGGCCCACACAGACACAATGTGACGGGGCGTACGCGGGGACCA
GCGTGGTGGTGACCGTGGGGGCCGCCGGGCAGCTGAGAGGCGTGCAGCTGTGGCGCGTGCCGGGCCCTGG
CCAGTATCTGATCTCAGCCTACGGAGCCGCGGGCGGCAAAGGCGCCAAGAACCACCTGTCGCGGGCGCAT
GGCGTCTTCGTCTCAGCAATCTTCTCCCTCGGTCTCGGGGAGTCGCTGTACATCCTGGTGGGGCAGCAGG
GAGAGGACGCCTGTCCCGGAGGTAGCCCGGAGAGCCAGCTCGTCTGCCTCGGGGAGTCTCGAGCCGTTGA
AGAGCACGCGGCGATGGATGGGAGCGAAGGGGTCCCGGGGTCGCGGCGCTGGGCGGGAGGTGGCGGGGGT
GGCGGGGGCGCCACCTACGTTTTCCGGGTGCGCGCTGGCGAGCTGGAACCGTTGCTGGTGGCGGCCGGAG
GCGGCGGTCGGGCCTACCTGAGGCCGCGGGACCGAGGCCGGACTCAGGCCTCCCCCGAGAAACTGGAGAA
CCGCTCGGAGGCGCCCGGGAGCGGCGGGAGAGGCGGGGCGGCAGGTGGTGGGGGCGGCTGGACGTCGCGG
GCTCCCTCTCCGCAGGCCGGCCGCTCACTGCAGGAGGGGGCGGAGGGCGGCCAGGGCTGCTCCGAGGCTT
GGGCGACCCTTGGCTGGGCCGCGGCCGGCGGCTTCGGGGGCGGCGGCGGGGCCTGCACTGCGGGCGGAGG
CGGCGGCGGCTACAGGGGGGGCGACGCTTCAGAGACTGACAACCTCTGGGCTGATGGGGAAGATGGAGTA
TCCTTCATACACCCCAGCAGCGAGCTCTTCCTGCAGCCTCTGGCAGTCACCGAGAACCACGGAGAGGTAG
AGATCCGAAGGCACCTCAACTGCAGTCACTGCCCTTTGAGAGACTGCCAATGGCAGGCAGAGCTCCAGCT
GGCTGAATGCCTGTGCCCAGAAGGCATGGAGCTAGCTGTGGATAACGTCACCTGCATGGACCTGCACAAG
CCCCCAGGCCCT

ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
TGGTACCGATATCAAGCTTGTCGACTCTAGA
Protein Sequence
>RC600053 representing signal peptide plus the extracellular domain region of NM_002344
Red=Cloning sites Green= DDK and 6XHIS Tags

MGCWGQLLVWFGAAGAILCSSPGSQETFLRSSPLPLASPSPRDPKVSAPPSILEPASPLNSPGTEGSWLF
STCGASGRHGPTQTQCDGAYAGTSVVVTVGAAGQLRGVQLWRVPGPGQYLISAYGAAGGKGAKNHLSRAH
GVFVSAIFSLGLGESLYILVGQQGEDACPGGSPESQLVCLGESRAVEEHAAMDGSEGVPGSRRWAGGGGG
GGGATYVFRVRAGELEPLLVAAGGGGRAYLRPRDRGRTQASPEKLENRSEAPGSGGRGGAAGGGGGWTSR
APSPQAGRSLQEGAEGGQGCSEAWATLGWAAAGGFGGGGGACTAGGGGGGYRGGDASETDNLWADGEDGV
SFIHPSSELFLQPLAVTENHGEVEIRRHLNCSHCPLRDCQWQAELQLAECLCPEGMELAVDNVTCMDLHK
PPGP

TRSGTRSGDYKDDDDKGSHHHHHH
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_002344
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002344.5, NP_002335.2
RefSeq Size 3143 bp
RefSeq ORF 2595 bp
Locus ID 4058
UniProt ID P29376
Cytogenetics 15q15.1
Protein Families Druggable Genome, Protein Kinase, Transmembrane
MW 42.9 kDa
Summary The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2008
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"NM_002344" in other vectors (7)
SKU Description Size Price
RC214572 LTK (Myc-DDK-tagged)-Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1 10 ug
$791.00
RC214572L1 Lenti ORF clone of Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1, Myc-DDK-tagged 10 ug
$1,091.00
RC214572L2 Lenti ORF clone of Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1, mGFP tagged 10 ug
$1,091.00
RC214572L3 Lenti ORF clone of Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1, Myc-DDK-tagged 10 ug
$1,091.00
RC214572L4 Lenti ORF clone of Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1, mGFP tagged 10 ug
$1,091.00
RG214572 LTK (tGFP-tagged) - Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1 10 ug
$991.00
SC303183 LTK (untagged)-Human leukocyte receptor tyrosine kinase (LTK), transcript variant 1 10 ug
$871.00

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