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pCMV6-XL5-DDK-His Mammalian Expression Vector

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SKU
PS100068
pCMV6-XL5-DDK-His, mammalian vector with C-terminal DDK-His tag (No mammalian selection marker), 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag DDK-His
E. coli Selection Ampicillin (100 ug/mL)
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be used for transient expression of a fusion protein with a C-terminal DDK-His tag.
  • Such clones are the best for detection and purification of the transgene expression using anti-DDK or anti-His antibodies or a nickel affinity columns.
  • OriGene's Extracellular Domain clones are housed in this vector.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Connexin 30 expression inhibits growth of human malignant gliomas but protects them against radiation therapy
Artesi, Kroonen, Bredel et al
Neuro-oncology (2014)
Cited by 45
AI Summary: OriGene’s pCMV6-XL5 plasmid was used to express Connexin 30 (Cx30) in glioblastoma cells, enabling the study of Cx30’s effects on tumor growth and response to radiation therapy in this research.
Snippet: Appropriate quantities of pCMV6-XL5 plasmid (Origene Technologies) encoding or not for Cx30 DNA (Clongen) and dissolved in TansIT LT1 transfection reagent (Mirus Bio) were added to the cells according to the manufacturer's instructions. ... Cite SaveSaving
Distinct fcγ receptors mediate the effect of serum amyloid p on neutrophil adhesion and fibrocyte differentiation
Cox, Pilling, Gomer
J. Immunol. (2014) 193 (4), 1701-8 DOI: 10.4049/jimmunol.1400281
Cited by 44
AI Summary: OriGene’s pCMV6-AC-His vector was used to express FcγRI and Fcε common γ-chain in HEK293 cells, enabling the study of SAP binding and receptor function in this research.
Snippet: SAP affinity assays and receptor expression FcgRI and Fcε common g-chain (FcRg) cDNA (PSI:Biology-materials re- pository, Tempe, AZ) (32, 33) were ligated into the pCMV6-AC-His vec- tor (OriGene, Rockville, MD) and then transfected into HEK293 cells using ... Cite SaveSaving
Showing 1-2 of 2 papers.
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