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SETMAR (NM_006515) Human Tagged ORF Clone

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SKU
RC229337
SETMAR (Myc-DDK-tagged)-Human SET domain and mariner transposase fusion gene (SETMAR)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $626.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SETMAR
Synonyms Mar1; METNASE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229337 representing NM_006515
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCGCGGAAGCGGCAAAGACGACACGGCCTTGTGGGATGGCGGAGTTTAAGGAGAAGCCTGAGGCCC
CGACTGAGCAGCTGGATGTCGCGTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCCGC
GCCGGCGCCCTTCCAGTACACTCCTGATCATGTAGTTGGACCTGGAGCAGACATTGATCCCACTCAAATA
ACCTTTCCCGGATGCATTTGTGTCAAAACTCCCTGCCTCCCTGGCACTTGCTCCTGTCTCCGCCATGGAG
AGAACTATGATGATAACTCATGCCTTAGAGATATAGGATCTGGAGGAAAGTATGCAGAGCCTGTTTTTGA
ATGCAATGTCCTGTGCCGATGCAGTGACCACTGCAGAAACAGAGTGGTCCAGAAAGGTCTACAGTTCCAC
TTCCAAGTGTTCAAGACGCATAAAAAAGGCTGGGGACTTCGTACCTTGGAATTTATACCGAAAGGAAGGT
TTGTCTGTGAATATGCTGGTGAGGTTTTAGGATTCTCTGAAGTTCAGAGAAGAATTCACTTACAAACAAA
ATCCGACTCCAATTACATTATAGCCATCAGGGAACATGTTTATAATGGGCAGGTAATGGAAACATTTGTT
GACCCTACTTATATAGGAAATATTGGAAGATTCCTTAATCATTCTTGTGAGCCAAACCTTTTGATGATTC
CTGTCCGAATTGACTCAATGGTACCTAAGTTGGCACTTTTTGCAGCCAAAGATATTGTGCCAGAAGAAGA
ACTCTCTTATGATTATTCAGGAAGATATCTTAATCTAACAGTCAGTGAAGACAAAGAAAGGCTAGATCAT
GGGAAACTAAGGAAACCTTGTTACTGTGGTGCCAAATCATGTACTGCTTTCCTGCCTTTTGACAGTTCTC
TGTACTGCCCCGTAGAAAAGTCGAACATCAGTTGTGGAAATGAGAAGGAACCCAGCATGTGTGGCTCAGC
CCCTTCTGTGTTCCCCTCCTGCAAGCGATTGACCCTTGAGACTATGAAAATGATGTTAGACAAAAAGCAA
ATTCGAGCAATTTTCTTATTCGAGTTCAAAATGGGTCGTAAAGCAGCAGAAACAACTCGCAACATCAACA
ATGCATTTGGCCCAGGAACTGCTAACGAACGTACAGTGCAGTGGTGGTTCAAGAAGTTTTGCAAAGGAGA
TGAGAGCCTTGAAGATGAGGAGCGTAGTGGCCGGCCATCAGAAGTTGACAACGACCAGTTGAGAGCAATC
ATCGAAGCTGATCCCCTTACAACTACACGAGAAGTTGCTGAAGAACTCAATGTCAACCATTCTACGGTCG
TTCGACATTTGAAGCAAATTGGAAAGGTGAAAAAGCTCGATAAGTGGGTGCCTCATGAGCTGACTGAAAA
TCAAAAAAATCGTCGTTTTGAAGTGTCATCTTCTCTTATTCTACGCAACCACAACGAACCATTTCTCGAT
CGGATTGTGACGTGTGATGAAAAGTGGATTTTATATGACAACCGGCGACGATCAGCTCAGTGGTTGGATC
AAGAAGAAGCTCCAAAGCACTTCCCAAAGCCAATCTTGCACCCAAAAAAGGTCATGGTCACTATTTGGTG
GTCTGCTGCTGGTCTGATCCACTACAGCTTTCTGAATCCCGGTGAAACCATTACATCTGAGAAGTATGCT
CAGGAAATCGATGAGATGAACCAAAAACTGCAACGCCTGCAGCTGGCATTGGTCAACAGAAAGGGCCCAA
TTCTTCTCCACGACAATGCCCGACCGCATGTTGCACAACCCACACTTCAAAAGTTGAATGAATTGGGCTA
TGAAGTTTTGCCTCATCCACCGTATTCACCTGACCTCTTGCCAACCAACTACCACGTCTTTAAGCATCTC
AACAACTTTTTGCAGGGAAAACGCTTCCACAACCAGCAGGATGCAGAAAATGCTTTCCAAGAGTTCGTCG
AATCCCAAAGCACGGATTTTTACGCTACAGGAATAAACCAACTTATTTCTCGTTGGCAAAAATGTGTTGA
TTGTAATGGTTCCTATTTTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC229337 representing NM_006515
Red=Cloning site Green=Tags(s)

MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQI
TFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFH
FQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFV
DPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDH
GKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLETMKMMLDKKQ
IRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAI
IEADPLTTTREVAEELNVNHSTVVRHLKQIGKVKKLDKWVPHELTENQKNRRFEVSSSLILRNHNEPFLD
RIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYA
QEIDEMNQKLQRLQLALVNRKGPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHL
NNFLQGKRFHNQQDAENAFQEFVESQSTDFYATGINQLISRWQKCVDCNGSYFD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006515
ORF Size 2052 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006515.3, NP_006506.3
RefSeq ORF 2055 bp
Locus ID 6419
UniProt ID Q53H47, E7EN68
Cytogenetics 3p26.1
Domains Pre-SET, PreSET, SET, Transposase_1
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Lysine degradation
MW 77.9 kDa
Summary This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. provided by RefSeq, Jan 2013
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"NM_006515" in other vectors (7)
SKU Description Size Price
RC229337L1 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), Myc-DDK-tagged 10 ug
$926.00
RC229337L2 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), mGFP tagged 10 ug
$926.00
RC229337L3 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), Myc-DDK-tagged 10 ug
$926.00
RC229337L4 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), mGFP tagged 10 ug
$926.00
RG229337 SETMAR (tGFP-tagged) - Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$826.00
SC314383 SETMAR (untagged)-Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$690.00
SC327972 SETMAR (untagged)-Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$690.00

Plasmid Map for RC229337

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC229337
Circular map for RC229337

file-alt Citations

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