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SETMAR (NM_006515) Human Untagged Clone

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SKU
SC327972
SETMAR (untagged)-Human SET domain and mariner transposase fusion gene (SETMAR)
  $690.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SETMAR
Synonyms Mar1; METNASE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC327972 representing NM_006515.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTCGCGGAAGCGGCAAAGACGACACGGCCTTGTGGGATGGCGGAGTTTAAGGAGAAGCCTGAGGCC
CCGACTGAGCAGCTGGATGTCGCGTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCC
GCGCCGGCGCCCTTCCAGTACACTCCTGATCATGTAGTTGGACCTGGAGCAGACATTGATCCCACTCAA
ATAACCTTTCCCGGATGCATTTGTGTCAAAACTCCCTGCCTCCCTGGCACTTGCTCCTGTCTCCGCCAT
GGAGAGAACTATGATGATAACTCATGCCTTAGAGATATAGGATCTGGAGGAAAGTATGCAGAGCCTGTT
TTTGAATGCAATGTCCTGTGCCGATGCAGTGACCACTGCAGAAACAGAGTGGTCCAGAAAGGTCTACAG
TTCCACTTCCAAGTGTTCAAGACGCATAAAAAAGGCTGGGGACTTCGTACCTTGGAATTTATACCGAAA
GGAAGGTTTGTCTGTGAATATGCTGGTGAGGTTTTAGGATTCTCTGAAGTTCAGAGAAGAATTCACTTA
CAAACAAAATCCGACTCCAATTACATTATAGCCATCAGGGAACATGTTTATAATGGGCAGGTAATGGAA
ACATTTGTTGACCCTACTTATATAGGAAATATTGGAAGATTCCTTAATCATTCTTGTGAGCCAAACCTT
TTGATGATTCCTGTCCGAATTGACTCAATGGTACCTAAGTTGGCACTTTTTGCAGCCAAAGATATTGTG
CCAGAAGAAGAACTCTCTTATGATTATTCAGGAAGATATCTTAATCTAACAGTCAGTGAAGACAAAGAA
AGGCTAGATCATGGGAAACTAAGGAAACCTTGTTACTGTGGTGCCAAATCATGTACTGCTTTCCTGCCT
TTTGACAGTTCTCTGTACTGCCCCGTAGAAAAGTCGAACATCAGTTGTGGAAATGAGAAGGAACCCAGC
ATGTGTGGCTCAGCCCCTTCTGTGTTCCCCTCCTGCAAGCGATTGACCCTTGAGACTATGAAAATGATG
TTAGACAAAAAGCAAATTCGAGCAATTTTCTTATTCGAGTTCAAAATGGGTCGTAAAGCAGCAGAAACA
ACTCGCAACATCAACAATGCATTTGGCCCAGGAACTGCTAACGAACGTACAGTGCAGTGGTGGTTCAAG
AAGTTTTGCAAAGGAGATGAGAGCCTTGAAGATGAGGAGCGTAGTGGCCGGCCATCAGAAGTTGACAAC
GACCAGTTGAGAGCAATCATCGAAGCTGATCCCCTTACAACTACACGAGAAGTTGCTGAAGAACTCAAT
GTCAACCATTCTACGGTCGTTCGACATTTGAAGCAAATTGGAAAGGTGAAAAAGCTCGATAAGTGGGTG
CCTCATGAGCTGACTGAAAATCAAAAAAATCGTCGTTTTGAAGTGTCATCTTCTCTTATTCTACGCAAC
CACAACGAACCATTTCTCGATCGGATTGTGACGTGTGATGAAAAGTGGATTTTATATGACAACCGGCGA
CGATCAGCTCAGTGGTTGGATCAAGAAGAAGCTCCAAAGCACTTCCCAAAGCCAATCTTGCACCCAAAA
AAGGTCATGGTCACTATTTGGTGGTCTGCTGCTGGTCTGATCCACTACAGCTTTCTGAATCCCGGTGAA
ACCATTACATCTGAGAAGTATGCTCAGGAAATCGATGAGATGAACCAAAAACTGCAACGCCTGCAGCTG
GCATTGGTCAACAGAAAGGGCCCAATTCTTCTCCACGACAATGCCCGACCGCATGTTGCACAACCCACA
CTTCAAAAGTTGAATGAATTGGGCTATGAAGTTTTGCCTCATCCACCGTATTCACCTGACCTCTTGCCA
ACCAACTACCACGTCTTTAAGCATCTCAACAACTTTTTGCAGGGAAAACGCTTCCACAACCAGCAGGAT
GCAGAAAATGCTTTCCAAGAGTTCGTCGAATCCCAAAGCACGGATTTTTACGCTACAGGAATAAACCAA
CTTATTTCTCGTTGGCAAAAATGTGTTGATTGTAATGGTTCCTATTTTGATTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_006515
Insert Size 2055 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006515.3
RefSeq Size 2157 bp
RefSeq ORF 2055 bp
Locus ID 6419
UniProt ID Q53H47
Cytogenetics 3p26.1
Domains Pre-SET, PreSET, SET, Transposase_1
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Lysine degradation
MW 78 kDa
Summary This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. provided by RefSeq, Jan 2013
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_006515" in other vectors (7)
SKU Description Size Price
RC229337 SETMAR (Myc-DDK-tagged)-Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$626.00
RC229337L1 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), Myc-DDK-tagged 10 ug
$926.00
RC229337L2 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), mGFP tagged 10 ug
$926.00
RC229337L3 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), Myc-DDK-tagged 10 ug
$926.00
RC229337L4 Lenti ORF clone of Human SET domain and mariner transposase fusion gene (SETMAR), mGFP tagged 10 ug
$926.00
RG229337 SETMAR (tGFP-tagged) - Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$826.00
SC314383 SETMAR (untagged)-Human SET domain and mariner transposase fusion gene (SETMAR) 10 ug
$690.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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