RFPL2 (NM_001098527) Human Tagged ORF Clone

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SKU
RC229180
RFPL2 (Myc-DDK-tagged)-Human ret finger protein-like 2 (RFPL2), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RFPL2
Synonyms RNF79
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229180 representing NM_001098527
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCTGAATTAGGCTTCCCAGAGACTGCAGTGTCCCAATCCAGGATCTGTCTATGTGCTGTAT
TGTGTGGCCACTGGGACTTTGCAGACATGATGGTGATAAGGAGCCTGAGCCTCATCAGGTTAGAGGGTGT
GGAAGGGCGTGACCCAGTGGGAGGTGGGAATCTCACCAATAAAAGGCCCTCGTGTGCCCCTTCCCCACAA
GACCTGAGCGCCCAGTGGAAGCAGCTGGAGGACAGAGGAGCTTCCAGCAGAAGAGTGGACATGGCTGCAC
TCTTCCAAGAAGCAAGCAGCTGTCCCGTCTGCTCAGACTATCTGGAAAAACCAATGTCCCTGGAGTGTGG
ATGCGCCGTCTGCCTCAAGTGCATTAATTCACTGCAGAAGGAGCCCCATGGGGAGGATCTACTTTGCTGT
TGCTCTTCCATGGTCTCTCGGAAGAACAAAATCAGGCGCAATCGGCAGCTAGAGAGGCTGGCTTCCCACA
TCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTGCAGATGAACCCAAGGATGCGGAAGTTCCAAGTGGA
TATGACCTTGGATGCCAACACAGCCAACAACTTCCTCCTCATTTCTGACGACCTCAGGAGCGTCCGAAGT
GGGCGCATCAGACAGAATCGGCAAGACCTTGCCGAGAGATTTGACGTGTCCGTTTGCATCCTGGGCTCCC
CTCGCTTTACCTGTGGCCGCCACTGCTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGT
CTGCAGAGAATCTGTTCACCGCAAAGGGAGGATCCAGCTGACCACAGAGCTTGGATTCTGGACTGTGAGT
TTGAGGGATGGAGGCCGCCTCTCTGCCACCACGGTGCCGCTGACTTTCCTCTTCGTAGACCGCAAGTTAC
AGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGATGCTGAAAGTGGTTCCCA
TGTCTATACATTCAGGAGCGTATCTGCTGAGGAGCCATTGCGCCCATTTTTGGCTCCTTCAGTTCCACCT
AATGGTGATCAAGGTGTCTTGAGCATCTGTCCTTTGATGAACTCAGGCACTACTGATGCTCCAGTCCGTC
CTGGGGAGGCCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229180 representing NM_001098527
Red=Cloning site Green=Tags(s)

MEVAELGFPETAVSQSRICLCAVLCGHWDFADMMVIRSLSLIRLEGVEGRDPVGGGNLTNKRPSCAPSPQ
DLSAQWKQLEDRGASSRRVDMAALFQEASSCPVCSDYLEKPMSLECGCAVCLKCINSLQKEPHGEDLLCC
CSSMVSRKNKIRRNRQLERLASHIKELEPKLKKILQMNPRMRKFQVDMTLDANTANNFLLISDDLRSVRS
GRIRQNRQDLAERFDVSVCILGSPRFTCGRHCWEVDVGTSTEWDLGVCRESVHRKGRIQLTTELGFWTVS
LRDGGRLSATTVPLTFLFVDRKLQRVGIFLDMGMQNVSFFDAESGSHVYTFRSVSAEEPLRPFLAPSVPP
NGDQGVLSICPLMNSGTTDAPVRPGEAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001098527
ORF Size 1134 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001098527.3
RefSeq ORF 1137 bp
Locus ID 10739
UniProt ID O75678
Cytogenetics 22q12.3
Protein Families Druggable Genome
MW 41.9 kDa
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Documents
Resources
"NM_001098527" in other vectors (5)
SKU Description Size Price
RC229180L3 Lenti ORF clone of Human ret finger protein-like 2 (RFPL2), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC229180L4 Lenti ORF clone of Human ret finger protein-like 2 (RFPL2), transcript variant 2, mGFP tagged 10 ug
$757.00
RG229180 RFPL2 (tGFP-tagged) - Human ret finger protein-like 2 (RFPL2), transcript variant 2 10 ug
$657.00
SC316355 RFPL2 (untagged)-Human ret finger protein-like 2 (RFPL2), transcript variant 2 10 ug
$503.00
SC327815 RFPL2 (untagged)-Human ret finger protein-like 2 (RFPL2) transcript variant 2 10 ug
$503.00

Plasmid Map for RC229180

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Cloning scheme for RC229180

Circular map for RC229180

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