PCBP3 (NM_020528) Human Tagged ORF Clone

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SKU
RC229176
PCBP3 (Myc-DDK-tagged)-Human poly(rC) binding protein 3 (PCBP3), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCBP3
Synonyms ALPHA-CP3; PCBP3-OT1; PCBP3OT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229176 representing NM_020528
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAAGGTGACGCCTTCTGGGCCCCATCTGTCCTTCCTCACAGCACCCTCAGCACCTTAAGCCACC
ACCCTCAGCCACAATTTGGCAGAAGGATGGAGTCCAAGGTCTCAGAAGGTGGCCTGAATGTGACCCTCAC
CATCCGCCTGCTGATGCATGGAAAGGAAGTTGGAAGCATCATCGGGAAGAAAGGAGAAACTGTGAAGAAG
ATGCGTGAGGAGAGTGGTGCAAGGATCAACATCTCAGAGGGAAACTGCCCAGAGAGGATTGTGACCATCA
CAGGCCCCACAGACGCCATCTTCAAGGCCTTTGCCATGATCGCATACAAGTTTGAGGAGGATATCATCAA
CTCCATGAGCAACAGCCCTGCCACCAGCAAGCCCCCAGTGACGCTGAGGCTGGTGGTGCCTGCCAGCCAG
TGTGGGTCCCTGATCGGCAAAGGAGGCTCCAAGATCAAGGAGATCAGGGAGTCCACAGGTGCCCAGGTGC
AGGTGGCTGGGGACATGCTGCCCAACTCCACGGAGCGAGCGGTGACCATCTCGGGGACCCCAGATGCCAT
CATCCAGTGCGTCAAGCAGATCTGTGTGGTCATGCTGGAGTCCCCACCGAAAGGTGCCACCATTCCCTAC
CGCCCAAAGCCCGCCTCCACCCCTGTCATTTTTGCAGGTGGTCAGGCCTACACAATCCAGGGACAGTATG
CCATCCCTCACCCGGATCAGTTGACCAAGCTCCACCAGTTGGCCATGCAGCAAACCCCCTTTCCTCCCCT
CGGACAGACCAACCCCGCTTTCCCCGGAGAAAAGCTGCCTTTACACTCCTCCGAAGAAGCTCAAAATCTG
ATGGGCCAGTCATCAGGTCTGGACGCCAGCCCACCGGCCAGCACTCATGAGCTCACCATTCCCAATGATC
TAATAGGCTGCATAATTGGACGCCAAGGGACCAAAATCAATGAAATTCGACAGATGTCTGGAGCTCAGAT
CAAAATCGCCAACGCCACGGAAGGGTCCTCAGAGCGTCAGATCACCATCACGGGGACCCCGGCCAACATC
AGCCTTGCCCAGTATCTCATCAACGCCAGGCTGACGTCCGAGGTCACCGGGATGGGCACGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229176 representing NM_020528
Red=Cloning site Green=Tags(s)

MGEGDAFWAPSVLPHSTLSTLSHHPQPQFGRRMESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKK
MREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQ
CGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLESPPKGATIPY
RPKPASTPVIFAGGQAYTIQGQYAIPHPDQLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNL
MGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANI
SLAQYLINARLTSEVTGMGTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020528
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020528.3
RefSeq ORF 1116 bp
Locus ID 54039
UniProt ID P57721
Cytogenetics 21q22.3
Domains KH
MW 39.3 kDa
Summary This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. The protein encoded by this gene lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jan 2017
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Documents
Resources
"NM_020528" in other vectors (5)
SKU Description Size Price
RC229176L3 Lenti ORF clone of Human poly(rC) binding protein 3 (PCBP3), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC229176L4 Lenti ORF clone of Human poly(rC) binding protein 3 (PCBP3), transcript variant 1, mGFP tagged 10 ug
$757.00
RG229176 PCBP3 (tGFP-tagged) - Human poly(rC) binding protein 3 (PCBP3), transcript variant 1 10 ug
$657.00
SC113092 PCBP3 (untagged)-Human poly(rC) binding protein 3 (PCBP3), transcript variant 1 10 ug
$457.00
SC327811 PCBP3 (untagged)-Human poly(rC) binding protein 3 (PCBP3) transcript variant 1 10 ug
$503.00

Plasmid Map for RC229176

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Cloning scheme for RC229176

Circular map for RC229176

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