SYNC (NM_001161708) Human Tagged ORF Clone

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SKU
RC228394
SYNC (Myc-DDK-tagged)-Human syncoilin, intermediate filament protein (SYNC), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SYNC
Synonyms SYNC1; SYNCOILIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228394 representing NM_001161708
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCCGGAGCCCCGGCGCGGCGGGGACGGCGCCGCCCAGGCCGCGAGGAAAACAAGAGTAGAGG
CCAATTCTCCTCTTCCAAAGAACTCTGGATCCCTAAATGAGGCAGAAGCCTTGAACCCAGAAGTTACTCT
ATCTTCAGAGGGGTCCTTAAACCTCGAAGACATTCTCTACCTGGAGGACACAGGTGACCTTGATGAGACA
CTCTATGTGCAAGAGACTGAGAAGGCAGAGGAGGCCCTGTATATTGAGGAGGCCATGCAGCCAGATGAGG
CTCTGCATGTGGAGGAGCCTGGGAATCCAGAGGAGACAGTGTGTGTGGAGGAAACCACGGAGCCAGATCG
GATACAGTTTGTGGAGGGGCCCGTGGAGCCAGGAAAGCCCACAAGCCCAGAGCACGTTGTTTATGAGGGA
GAGACAGTCACAAGGGCGGAGAAATCTAACCCTGAGGAGAGCCTCAGAGCCGAGCAGAGCCCCAGCGTGG
AGGAGAACCTGAGCATAGAGGACCTGGAATTGCTAGAGGGGCGTTTCCAGCAGTGTGTCCAAGCTGTGGC
CCAGCTGGAAGAGGAGAGGGATCAGCTCATCCATGAGCTTGTATTGCTCCGGGAACCAGCCCTGCAGGAG
GTACAGCAAGTCCATCAAGACATCCTGGCTGCCTACAAGCTCCATGCCCAAGCAGAGCTGGAGAGAGATG
GCCTAAGGGAGGAGATCCGGCTGGTCAAGCAGAAGCTTTTCAAAGTGACAAAGGAATGTGTGGCCTACCA
ATACCAGCTGGAGTGCCGCCAGCAGGACGTGGCTCAGTTTGCCGATTTCCGGGAAGTGCTGACTACAAGG
GCAACCCAGCTCTCAGAGGAACTGGCCCAGCTCCGGGATGCCTATCAGAAGCAGAAGGAGCAGCTGCGGC
AACAACTAGAAGCCCCTCCAAGCCAGAGGGATGGGCACTTTCTCCAGGAAAGCCGGCGACTCTCTGCCCA
GTTTGAAAATCTCATGGCAGAGAGCCGCCAGGACCTGGAGGAGGAGTATGAGCCTCAGTTCCTGCGGCTC
CTAGAGAGGAAAGAAGCTGGGACCAAAGCTCTGCAGAGAACCCAGGCTGAGATCCAGGAAATGAAGGAGG
CTCTGAGACCCCTGCAAGCAGAGGCCCGGCAGCTCCGCCTGCAAAACAGGAACCTGGAGGACCAGATCGC
ACTTGTGAGGCAAAAACGAGATGAAGAGGTGCAGCAGTACAGGGAACAGCTGGAGGAAATGGAAGAACGC
CAGAGGCAGTTAAGAAATGGGGTGCAACTCCAGCAACAGAAGAACAAAGAGATGGAACAGCTAAGGCTCA
GTCTTGCTGAAGAGCTCTCTACTTATAAGGGCTGTTTAGAAATATATGGCCAAATCTGTAACCCGGAAAC
AGCAAAAAACTTCTTAGCAAAGGATCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC228394 representing NM_001161708
Red=Cloning site Green=Tags(s)

MASPEPRRGGDGAAQAARKTRVEANSPLPKNSGSLNEAEALNPEVTLSSEGSLNLEDILYLEDTGDLDET
LYVQETEKAEEALYIEEAMQPDEALHVEEPGNPEETVCVEETTEPDRIQFVEGPVEPGKPTSPEHVVYEG
ETVTRAEKSNPEESLRAEQSPSVEENLSIEDLELLEGRFQQCVQAVAQLEEERDQLIHELVLLREPALQE
VQQVHQDILAAYKLHAQAELERDGLREEIRLVKQKLFKVTKECVAYQYQLECRQQDVAQFADFREVLTTR
ATQLSEELAQLRDAYQKQKEQLRQQLEAPPSQRDGHFLQESRRLSAQFENLMAESRQDLEEEYEPQFLRL
LERKEAGTKALQRTQAEIQEMKEALRPLQAEARQLRLQNRNLEDQIALVRQKRDEEVQQYREQLEEMEER
QRQLRNGVQLQQQKNKEMEQLRLSLAEELSTYKGCLEIYGQICNPETAKNFLAKDH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001161708
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001161708.1, NP_001155180.1
RefSeq ORF 1431 bp
Locus ID 81493
UniProt ID Q9H7C4, Q9H7C4-2
Cytogenetics 1p35.1
MW 54.7 kDa
Summary This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2009
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Documents
Resources
"NM_001161708" in other vectors (4)
SKU Description Size Price
RC228394L3 Lenti ORF clone of Human syncoilin, intermediate filament protein (SYNC), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC228394L4 Lenti ORF clone of Human syncoilin, intermediate filament protein (SYNC), transcript variant 2, mGFP tagged 10 ug
$757.00
RG228394 SYNC (tGFP-tagged) - Human syncoilin, intermediate filament protein (SYNC), transcript variant 2 10 ug
$657.00
SC327029 SYNC (untagged)-Human syncoilin intermediate filament protein (SYNC) transcript variant 2 10 ug
$503.00

Plasmid Map for RC228394

Download sequence for

Cloning scheme for RC228394

Circular map for RC228394

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