OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TLE2 (NM_001144762) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC227899
TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $578.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA504240 TLE2 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6) 100 ul
$447.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "TLE2 or NM_001144762"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TLE2
Synonyms ESG; ESG2; GRG2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227899 representing NM_001144762
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGG
AGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCA
GGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGGGAGTGCA
TCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGTGGTGGCGGGAAGCAGA
GAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTGATTACAATCTGGTGGT
GGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAAGGTACCCATCTGCATT
CCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGCTCACCGCTGCCTAGAG
CCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCTGTGACTCCTCCCCGCC
CCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGCTGCCAAGCCAGCACCT
TCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACCACGTCCTTCAGCCTGG
GCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCCTCCACCTGTCCCCCCA
GGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCATCCCCATCTCCGAGGG
TCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCCTTCCACGTGTCTGCGG
ACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCATCCCGCGGCACGCCCG
GCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGGCTCCACACAGCATGTG
TACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCCAAGACGCCCGTGGCCC
AGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGGATGGCCGGAGTCTGAT
CGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCCCCGTATCAAGGCCGAG
CTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAGGTTTGCTTCTCCTGCT
GCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGCAGTTCCAGGGCCACAC
GGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGGGGGCCTGGACAACACG
GTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGCTCCCAGATTTTCTCCC
TGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACGTGGAGATCCTGCACGT
CCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCTGAAGTTTGCCTCCTGC
GGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGGACGCCGTACGGGGCCAGCA
TTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAAATAACAAATACATCGTGAC
AGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227899 representing NM_001144762
Red=Cloning site Green=Tags(s)

MQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGSA
SPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICI
PARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAP
STDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRG
SSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHV
YTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAE
LTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNT
VRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASC
GRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001144762
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001144762.1, NP_001138234.1
RefSeq ORF 1866 bp
Locus ID 7089
UniProt ID Q04725
Cytogenetics 19p13.3
Protein Families Transcription Factors
MW 66.5 kDa
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001144762" in other vectors (4)
SKU Description Size Price
RC227899L3 Lenti-ORF clone of TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$878.00
RC227899L4 Lenti-ORF clone of TLE2 (mGFP-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$878.00
RG227899 TLE2 (tGFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$778.00
SC326117 TLE2 (untagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$637.00

Plasmid Map for RC227899

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227899
Circular map for RC227899

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products