TLE2 (NM_001144762) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC227899L4
Lenti-ORF clone of TLE2 (mGFP-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3
  $878.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TLE2
Synonyms ESG; ESG2; GRG2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC227899).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001144762
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001144762.1, NP_001138234.1
RefSeq ORF 1866 bp
Locus ID 7089
UniProt ID Q04725
Cytogenetics 19p13.3
Protein Families Transcription Factors
MW 66.5 kDa
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001144762" in other vectors (4)
SKU Description Size Price
RC227899 TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$578.00
RC227899L3 Lenti-ORF clone of TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$878.00
RG227899 TLE2 (tGFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$778.00
SC326117 TLE2 (untagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3 10 ug
$637.00

Plasmid Map for RC227899L4

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Cloning scheme for RC227899L4

Circular map for RC227899L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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