OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

DYNC1I1 (NM_001135557) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC226891
DYNC1I1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $566.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA375665 DYNC1I1 Rabbit polyclonal Antibody
100 ul
$410.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "DYNC1I1 or NM_001135557"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DYNC1I1
Synonyms DNCI1; DNCIC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC226891 representing NM_001135557
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGACAAAAGTGACTTAAAAGCTGAGCTAGAGCGCAAAAAGCAGCGCTTAGCACAGATAAGAGAAG
AGAAGAAACGGAAGGAAGAGGAGAGGAAAAAGAAAGAGGCTGATATGCAGCAGAAGAAAGAACCCGTTCA
GGACGACTCTGATCTGGATCGCAAACGACGAGAGACAGAGGCTTTGCTGCAAAGCATTGGTATCTCACCG
GAGCCGCCTCTAGTCCCAACCCCTATGTCTCCCTCCTCGAAATCAGTGAGCACTCCCAGTGAAGCTGGAA
GCCAAGACTCAGGCGATCTGGGGCCATTAACAAGAAGAAGACTGCATAAACTGGGCGTGTCAAAGGTCAC
CCAAGTGGATTTCCTGCCAAGGGAAGTAGTGTCCTACTCAAAGGAGACCCAGACTCCTCTTGCCACGCAT
CAGTCTGAAGAGGATGAGGAAGATGAGGAAATGGTGGAATCTAAAGTTGGCCAGGACTCAGAACTGGAAA
ATCAGGACAAAAAACAGGAAGTGAAGGAAGCCCCTCCAAGAGAGTTGACAGAGGAAGAAAAACAGCAGAT
CCTTCATTCAGAGGAATTTCTCATCTTTTTTGACCGGACAATACGGGTAATTGAAAGAGCCCTGGCTGAA
GATTCCGACATCTTTTTTGACTACAGCGGCCGAGAGTTAGAGGAAAAAGATGGGGATGTTCAGGCTGGAG
CCAATCTTTCTTTCAATCGTCAGTTCTATGATGAACATTGGTCCAAGCATCGAGTGGTCACTTGTATGGA
CTGGTCCCTCCAGTACCCTGAGCTGATGGTGGCTTCTTACAACAACAATGAAGATGCTCCCCATGAACCA
GATGGAGTGGCCTTGGTTTGGAACATGAAGTTTAAGAAAACCACACCAGAATACGTCTTCCACTGTCAGT
CCTCTGTGATGTCGGTCTGCTTCGCCCGTTTCCATCCTAACTTGGTGGTTGGTGGGACTTACTCGGGCCA
GATTGTCCTCTGGGACAATCGCAGTCATCGAAGGACTCCAGTGCAGCGGACACCCTTATCAGCTGCTGCA
CACACGCATCCCGTGTACTGTGTAAATGTTGTTGGGACCCAGAATGCTCATAACCTCATCACTGTCTCCA
CTGATGGCAAAATGTGTTCCTGGAGCCTGGACATGCTCTCAACTCCACAGGAGAGCATGGAGCTGGTGTA
CAATAAGTCCAAGCCTGTCGCTGTTACCGGAATGGCTTTCCCAACGGGAGACGTCAATAACTTCGTGGTT
GGCAGTGAGGAAGGTACAGTCTACACGGCTTGTCGTCATGGAAGCAAAGCAGGTATTGGTGAGGTCTTTG
AAGGTCACCAAGGGCCAGTGACAGGAATTAACTGCCACATGGCAGTGGGCCCAATCGACTTTTCTCACCT
GTTTGTCACATCATCATTTGACTGGACTGTCAAACTGTGGACCACCAAGCACAACAAGCCGCTCTACTCC
TTTGAAGACAATGCAGACTATGTGTACGATGTCATGTGGTCCCCCGTGCATCCTGCGCTTTTTGCCTGCG
TGGACGGGATGGGGCGCTTGGACCTCTGGAACCTCAACAATGACACCGAGGTTCCAACAGCAAGTGTGGC
CATTGAGGGGGCATCCGCCCTAAACCGTGTTCGTTGGGCCCAAGCTGGCAAAGAAGTTGCTGTTGGGGAC
TCGGAAGGCCGTATTTGGGTCTATGACGTTGGAGAGCTTGCAGTTCCCCACAATGATGAATGGACCCGAT
TTGCCAGGACCCTTGTGGAAATTCGTGCTAACAGAGCTGATAGCGAGGAGGAAGGCACTGTTGAGTTATC
TGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC226891 representing NM_001135557
Red=Cloning site Green=Tags(s)

MSDKSDLKAELERKKQRLAQIREEKKRKEEERKKKEADMQQKKEPVQDDSDLDRKRRETEALLQSIGISP
EPPLVPTPMSPSSKSVSTPSEAGSQDSGDLGPLTRRRLHKLGVSKVTQVDFLPREVVSYSKETQTPLATH
QSEEDEEDEEMVESKVGQDSELENQDKKQEVKEAPPRELTEEEKQQILHSEEFLIFFDRTIRVIERALAE
DSDIFFDYSGRELEEKDGDVQAGANLSFNRQFYDEHWSKHRVVTCMDWSLQYPELMVASYNNNEDAPHEP
DGVALVWNMKFKKTTPEYVFHCQSSVMSVCFARFHPNLVVGGTYSGQIVLWDNRSHRRTPVQRTPLSAAA
HTHPVYCVNVVGTQNAHNLITVSTDGKMCSWSLDMLSTPQESMELVYNKSKPVAVTGMAFPTGDVNNFVV
GSEEGTVYTACRHGSKAGIGEVFEGHQGPVTGINCHMAVGPIDFSHLFVTSSFDWTVKLWTTKHNKPLYS
FEDNADYVYDVMWSPVHPALFACVDGMGRLDLWNLNNDTEVPTASVAIEGASALNRVRWAQAGKEVAVGD
SEGRIWVYDVGELAVPHNDEWTRFARTLVEIRANRADSEEEGTVELSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001135557
ORF Size 1824 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135557.1, NP_001129029.1
RefSeq ORF 1827 bp
Locus ID 1780
UniProt ID O14576, Q8N542, O14576-3
Cytogenetics 7q21.3
MW 68.4 kDa
Summary Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001135557" in other vectors (4)
SKU Description Size Price
RC226891L3 Lenti-ORF clone of DYNC1I1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$866.00
RC226891L4 Lenti-ORF clone of DYNC1I1 (mGFP-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$866.00
RG226891 DYNC1I1 (tGFP-tagged) - Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$766.00
SC326103 DYNC1I1 (untagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$624.00

Plasmid Map for RC226891

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC226891
Circular map for RC226891

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products