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DYNC1I1 (NM_001135557) Human Untagged Clone

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SKU
SC326103
DYNC1I1 (untagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3
  $624.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DYNC1I1
Synonyms DNCI1; DNCIC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC326103 representing NM_001135557.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTGACAAAAGTGACTTAAAAGCTGAGCTAGAGCGCAAAAAGCAGCGCTTAGCACAGATAAGAGAA
GAGAAGAAACGGAAGGAAGAGGAGAGGAAAAAGAAAGAGGCTGATATGCAGCAGAAGAAAGAACCCGTT
CAGGACGACTCTGATCTGGATCGCAAACGACGAGAGACAGAGGCTTTGCTGCAAAGCATTGGTATCTCA
CCGGAGCCGCCTCTAGTCCCAACCCCTATGTCTCCCTCCTCGAAATCAGTGAGCACTCCCAGTGAAGCT
GGAAGCCAAGACTCAGGCGATCTGGGGCCATTAACAAGAAGAAGACTGCATAAACTGGGCGTGTCAAAG
GTCACCCAAGTGGATTTCCTGCCAAGGGAAGTAGTGTCCTACTCAAAGGAGACCCAGACTCCTCTTGCC
ACGCATCAGTCTGAAGAGGATGAGGAAGATGAGGAAATGGTGGAATCTAAAGTTGGCCAGGACTCAGAA
CTGGAAAATCAGGACAAAAAACAGGAAGTGAAGGAAGCCCCTCCAAGAGAGTTGACAGAGGAAGAAAAA
CAGCAGATCCTTCATTCAGAGGAATTTCTCATCTTTTTTGACCGGACAATACGGGTAATTGAAAGAGCC
CTGGCTGAAGATTCCGACATCTTTTTTGACTACAGCGGCCGAGAGTTAGAGGAAAAAGATGGGGATGTT
CAGGCTGGAGCCAATCTTTCTTTCAATCGTCAGTTCTATGATGAACATTGGTCCAAGCATCGAGTGGTC
ACTTGTATGGACTGGTCCCTCCAGTACCCTGAGCTGATGGTGGCTTCTTACAACAACAATGAAGATGCT
CCCCATGAACCAGATGGAGTGGCCTTGGTTTGGAACATGAAGTTTAAGAAAACCACACCAGAATACGTC
TTCCACTGTCAGTCCTCTGTGATGTCGGTCTGCTTCGCCCGTTTCCATCCTAACTTGGTGGTTGGTGGG
ACTTACTCGGGCCAGATTGTCCTCTGGGACAATCGCAGTCATCGAAGGACTCCAGTGCAGCGGACACCC
TTATCAGCTGCTGCACACACGCATCCCGTGTACTGTGTAAATGTTGTTGGGACCCAGAATGCTCATAAC
CTCATCACTGTCTCCACTGATGGCAAAATGTGTTCCTGGAGCCTGGACATGCTCTCAACTCCACAGGAG
AGCATGGAGCTGGTGTACAATAAGTCCAAGCCTGTCGCTGTTACCGGAATGGCTTTCCCAACGGGAGAC
GTCAATAACTTCGTGGTTGGCAGTGAGGAAGGTACAGTCTACACGGCTTGTCGTCATGGAAGCAAAGCA
GGTATTGGTGAGGTCTTTGAAGGTCACCAAGGGCCAGTGACAGGAATTAACTGCCACATGGCAGTGGGC
CCAATCGACTTTTCTCACCTGTTTGTCACATCATCATTTGACTGGACTGTCAAACTGTGGACCACCAAG
CACAACAAGCCGCTCTACTCCTTTGAAGACAATGCAGACTATGTGTACGATGTCATGTGGTCCCCCGTG
CATCCTGCGCTTTTTGCCTGCGTGGACGGGATGGGGCGCTTGGACCTCTGGAACCTCAACAATGACACC
GAGGTTCCAACAGCAAGTGTGGCCATTGAGGGGGCATCCGCCCTAAACCGTGTTCGTTGGGCCCAAGCT
GGCAAAGAAGTTGCTGTTGGGGACTCGGAAGGCCGTATTTGGGTCTATGACGTTGGAGAGCTTGCAGTT
CCCCACAATGATGAATGGACCCGATTTGCCAGGACCCTTGTGGAAATTCGTGCTAACAGAGCTGATAGC
GAGGAGGAAGGCACTGTTGAGTTATCTGCCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001135557
Insert Size 1827 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135557.1
RefSeq Size 2950 bp
RefSeq ORF 1827 bp
Locus ID 1780
UniProt ID O14576
Cytogenetics 7q21.3
MW 68.6 kDa
Summary Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) uses an alternate in-frame splice junction and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.
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Documents
Resources
"NM_001135557" in other vectors (4)
SKU Description Size Price
RC226891 DYNC1I1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$566.00
RC226891L3 Lenti-ORF clone of DYNC1I1 (Myc-DDK-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$866.00
RC226891L4 Lenti-ORF clone of DYNC1I1 (mGFP-tagged)-Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$866.00
RG226891 DYNC1I1 (tGFP-tagged) - Human dynein, cytoplasmic 1, intermediate chain 1 (DYNC1I1), transcript variant 3 10 ug
$766.00

file-alt Citations

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