ENOSF1 (NM_001126123) Human Tagged ORF Clone

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SKU
RC225544
ENOSF1 (Myc-DDK-tagged)-Human enolase superfamily member 1 (ENOSF1), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ENOSF1
Synonyms RTS; TYMSAS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC225544 representing NM_001126123
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAAGATGGAATCAAGGGGTGTGGAATTACCTTCACTCTGGGAAAAGGCACTGAAGTTGATTGGTC
CAGAAAAGGGCGTGGTGCACCTGGCGACAGCGGCCGTCCTAAACGCGGTGTGGGACTTGTGGGCCAAGCA
GGAGGGAAAGCCTGTCTGGAAGTTACTTGTGGACATGGATCCCAGGATGCTGGTATCCTGCATAGATTTC
AGGTACATCACTGATGTCCTGACTGAGGAGGATGCCCTAGAAATACTGCAGAAAGGTCAAATTGGTAAAA
AAGAAAGAGAGAAGCAAATGCTGGCACAAGGATACCCTGCTTACACGACATCGTGCGCCTGGCTGGGGTA
CTCAGATGACACGTTGAAGCAGCTCTGTGCCCAGGCGCTGAAGGATGGCTGGACCAGGTTTAAAGTAAAG
GTGGGTGCTGATCTCCAGGATGACATGCGAAGATGCCAAATCATCCGAGACATGATTGGACCGGAAAAGA
CTTTGATGATGGATGCCAACCAGCGCTGGGATGTGCCTGAGGCGGTGGAGTGGATGTCCAAGCTGGCCAA
GTTCAAGCCATTGTGGATTGAGGAGCCAACCTCCCCTGATGACATTCTGGGGCACGCCACCATTTCCAAG
GCACTGGTCCCATTAGGAATTGGCATTGCCACAGGAGAACAGTGCCACAATAGAGTGATATTTAAGCAAC
TCCTACAGGCGAAGGCCCTGCAGTTCCTCCAGATTGACAGTTGCAGACTGGGCAGTGTCAATGAGAACCT
CTCAGTATTGCTGATGGCCAAAAAGTTTGAAATTCCTGTTTGCCCCCATGCTGGTGGAGTTGGCCTCTGT
GAACTGGTGCAGCACCTGATTATATTTGACTACATATCAGTTTCTGCAAGCCTTGAAAATAGGGTGTGTG
AGTATGTTGACCACCTGCATGAGCATTTCAAGTATCCCGTGATGATCCAGCGGGCTTCCTACATGCCTCC
CAAGGATCCCGGCTACTCAACAGAAATGAAGGAGGAATCTGTAAAGAAACACCAGTATCCAGATGGTGAA
GTTTGGAAGAAACTCCTTCCTGCTCAAGAAAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC225544 representing NM_001126123
Red=Cloning site Green=Tags(s)

MQKMESRGVELPSLWEKALKLIGPEKGVVHLATAAVLNAVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDF
RYITDVLTEEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTRFKVK
VGADLQDDMRRCQIIRDMIGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIEEPTSPDDILGHATISK
ALVPLGIGIATGEQCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKFEIPVCPHAGGVGLC
ELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKEESVKKHQYPDGE
VWKKLLPAQEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001126123
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001126123.3, NP_001119595.1
RefSeq ORF 1086 bp
Locus ID 55556
UniProt ID Q7L5Y1
Cytogenetics 18p11.32
MW 40.8 kDa
Summary This gene can encode a mitochondrial enzyme that is thought to convert L-fuconate to 2-keto-3-deoxy-L-fuconate. This locus was originally identified as the source of antisense RNAs of the adjacent thymidylate synthase gene. Splice variants at this locus may contain an alternate 3' exon that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and may downregulate TS expression. provided by RefSeq, Aug 2017
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Documents
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"NM_001126123" in other vectors (4)
SKU Description Size Price
RC225544L3 Lenti ORF clone of Human enolase superfamily member 1 (ENOSF1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC225544L4 Lenti ORF clone of Human enolase superfamily member 1 (ENOSF1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG225544 ENOSF1 (tGFP-tagged) - Human enolase superfamily member 1 (ENOSF1), transcript variant 2 10 ug
$657.00
SC323001 ENOSF1 (untagged)-Human enolase superfamily member 1 (ENOSF1), transcript variant 2 10 ug
$503.00

Plasmid Map for RC225544

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Cloning scheme for RC225544

Circular map for RC225544

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