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AP2 alpha (AP2A1) (NM_014203) Human Tagged ORF Clone

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SKU
RC224855
AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $894.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AP2 alpha
Synonyms ADTAA; AP2-ALPHA; CLAPA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224855 representing NM_014203
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATCCGGAACTGTA
AGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATCCGCTCCAAGTTCAAAGGAGA
CAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGTAAACTGCTTTTCATCTTCCTGCTTGGCCAT
GACATTGACTTTGGGCACATGGAGGCTGTGAATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAG
GTTACCTGTTCATTTCTGTGCTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAA
GAATGACCTGGCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGCATGGACAGTG
TCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCTGACCTGGTGCCCATGGGCGA
GTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCACATGGGTGTGGTCACGGCCGCCGTCAGCCTC
ATCACCTGTCTCTGCAAGAAGAACCCAGATGACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGA
GCCGGATCGTCTCCTCTGCCTCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCT
CTCGGTGAAGCTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTGCAGCATTCCA
ACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT
GGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACCAACCTGCGCTACCTGGCCCTG
GAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCA
TCAATGCCCTCAAGACGGAGCGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTG
TGACCGGAGCAATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGCTGGTACGTGG
ACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAGGTGTGGTACCGTGTGCTACA
GATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCCAAGACCGTCTTTGAGGCGCTCCAGGCCCCT
GCCTGTCACGAGAACATGGTGAAGGTTGGCGGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGG
ACCCCCGCTCCAGCCCCCCAGTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCAC
GCGGGCGCTGCTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCCGTGGAGTACC
TCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAGGAGATGCCGCCCTTCCCCGA
GCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAGGGGCCAGGGGCCGGCAGCGCCCTGGACGAT
GGCCGGAGGGACCCCAGCAGCAACGACATCAACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGC
CCTCGCCCTCCGCCGACCTCCTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGG
AGCAGGGAACCTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG
GAGGCCTTCCTCAGCGAGCTGGAGCCGCCTGCCCCCGAGAGCCCCATGGCTTTGCTGGCTGACCCAGCTC
CAGCTGCTGACCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCCGATGAGTTGCTGAATAAGTT
TGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTGCTGCAGATCGGAGTCAAGTCAGAGTTCCGA
CAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACCTCGGTGCAGTTCCAGAATTTCTCACCCA
CTGTGGTTCACCCGGGAGACCTCCAGACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGGTGGA
CGGCGGCGCGCAGGTGCAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTG
TCCGTGCGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACCATCAACAAGT
TCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAGCAGCTGAGCCTCCCTCAACA
GGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGACGCAGAAGTTACTAAGGCCAAGCTTCTGGGG
TTTGGCTCTGCTCTCCTGGACAATGTGGACCCCAACCCTGAGAACTTCGTGGGGGCGGGGATCATCCAGA
CTAAAGCCCTGCAGGTGGGCTGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGCTGAC
CCTGCGCACCAGCAAGGAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224855 representing NM_014203
Red=Cloning site Green=Tags(s)

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS
REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL
ITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL
VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL
ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP
ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ
GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDD
GRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPE
EAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFR
QNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLL
SVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLG
FGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014203
ORF Size 2931 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014203.3
RefSeq Size 3499 bp
RefSeq ORF 2934 bp
Locus ID 160
UniProt ID O95782
Cytogenetics 19q13.33
Domains Adaptin_N, Alpha_adaptinC2, Alpha_adaptin_C
Protein Pathways Endocytosis, Huntington's disease
MW 107.4 kDa
Summary This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_014203" in other vectors (4)
SKU Description Size Price
RC224855L3 Lenti-ORF clone of AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,194.00
RC224855L4 Lenti-ORF clone of AP2A1 (mGFP-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,194.00
RG224855 AP2A1 (tGFP-tagged) - Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,094.00
SC315023 AP2A1 (untagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$895.00

Plasmid Map for RC224855

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224855
Circular map for RC224855

file-alt Citations

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