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AP2 alpha (AP2A1) (NM_014203) Human Untagged Clone

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SKU
SC315023
AP2A1 (untagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1
  $895.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol AP2 alpha
Synonyms ADTAA; AP2-ALPHA; CLAPA1
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_014203 edited
ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATC
CGGAACTGTAAGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATC
CGCTCCAAGTTCAAAGGAGACAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGT
AAACTGCTTTTCATCTTCCTGCTTGGCCATGACATTGACTTTGGGCACATGGAGGCTGTG
AATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAGGTTACCTGTTCATTTCTGTG
CTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAAGAATGACCTG
GCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGC
ATGGACAGTGTCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCT
GACCTGGTGCCCATGGGCGAGTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCAC
ATGGGTGTGGTCACGGCCGCCGTCAGCCTCATCACCTGTCTCTGCAAGAAGAACCCAGAT
GACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGAGCCGGATCGTCTCCTCTGCC
TCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCTCTCGGTGAAG
CTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTG
CAGCATTCCAACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTAT
GACAGTGAGCCCAACCTCCTGGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCAC
CGGGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCCGAG
TTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCATCAATGCCCTCAAGACGGAG
CGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTGTGACCGGAGC
AATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTATAGC
TGGTACGTGGACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAG
GTGTGGTACCGTGTGCTACAGATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCC
AAGACCGTCTTTGAGGCGCTCCAGGCCCCTGCCTGTCACGAGAACATGGTGAAGGTTGGC
GGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGGACCCCCGCTCCAGCCCCCCA
GTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCACGCGGGCGCTG
CTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCC
GTGGAGTACCTCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAG
GAGATGCCGCCCTTCCCCGAGCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAG
GGGCCAGGGGCCGGCAGCGCCCTGGACGATGGCCGGAGGGACCCCAGCAGCAACGACATC
AACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGCCCTCGCCCTCCGCCGACCTC
CTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGGAGCAGGGAAC
CTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG
GAGGCCTTCCTCAGCGAGCTGGAGCCGCCTGCCCCCGAGAGCCCCATGGCTTTGCTGGCT
GACCCAGCTCCAGCTGCTGACCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCC
GATGAGTTGCTGAATAAGTTTGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTG
CTGCAGATCGGAGTCAAGTCAGAGTTCCGACAGAACCTGGGCCGCATGTATCTCTTCTAT
GGCAACAAGACCTCGGTGCAGTTCCAGAATTTCTCACCCACTGTGGTTCACCCGGGAGAC
CTCCAGACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGGTGGACGGCGGCGCG
CAGGTGCAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTG
TCCGTGCGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACC
ATCAACAAGTTCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAG
CAGCTGAGCCTCCCTCAACAGGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGAC
GCAGAAGTTACTAAGGCCAAGCTTCTGGGGTTTGGCTCTGCTCTCCTGGACAATGTGGAC
CCCAACCCTGAGAACTTCGTGGGGGCGGGGATCATCCAGACTAAAGCCCTGCAGGTGGGC
TGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGCTGACCCTGCGCACC
AGCAAGGAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTCTGA
Restriction Sites Please inquire |
ACCN NM_014203
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this clone has been fully sequenced and found one SNP within the protein associated with this reference, NM_014203.2. This SNP doesn't change amino acid.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014203.2, NP_055018.2
RefSeq Size 3499 bp
RefSeq ORF 2934 bp
Locus ID 160
UniProt ID O95782
Cytogenetics 19q13.33
Domains Adaptin_N, Alpha_adaptinC2, Alpha_adaptin_C
Protein Pathways Endocytosis, Huntington's disease
Summary This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the longer isoform of this protein.
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"NM_014203" in other vectors (4)
SKU Description Size Price
RC224855 AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$894.00
RC224855L3 Lenti-ORF clone of AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,194.00
RC224855L4 Lenti-ORF clone of AP2A1 (mGFP-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,194.00
RG224855 AP2A1 (tGFP-tagged) - Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1 10 ug
$1,094.00

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