NRDE2 (NM_017970) Human Tagged ORF Clone
- TrueORF®
SKU
RC224847
NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1
$1,520.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NRDE2 |
| Synonyms | C14orf102 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017970 |
| ORF Size | 3492 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017970.4 |
| RefSeq Size | 3824 bp |
| RefSeq ORF | 3495 bp |
| Locus ID | 55051 |
| UniProt ID | Q9H7Z3 |
| Cytogenetics | 14q32.11 |
| MW | 132.5 kDa |
| Summary | Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation (PubMed:30842217). Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation (PubMed:30842217). Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome (PubMed:30842217). By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm (PubMed:30842217).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017970" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC224847L1 | Lenti-ORF clone of NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,820.00
|
|
| RC224847L2 | Lenti-ORF clone of NRDE2 (mGFP-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,820.00
|
|
| RC224847L3 | Lenti-ORF clone of NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,820.00
|
|
| RC224847L4 | Lenti-ORF clone of NRDE2 (mGFP-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,820.00
|
|
| RG224847 | NRDE2 (tGFP-tagged) - Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,720.00
|
|
| SC304549 | NRDE2 (untagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 | 10 ug |
$1,622.00
|
|
Citations
Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor
Jiao, Perales, Umbreit et al
RNA (2019) 25 (3), 352-363 DOI: 10.1261/rna.069773.118
Jiao, Perales, Umbreit et al
RNA (2019) 25 (3), 352-363 DOI: 10.1261/rna.069773.118
Cited by 19
AI Summary: In this study, OriGene products including NRDE2 shRNA (OriGene, Cat# TL313919) and NRDE2 cDNA clone (OriGene, Cat# RC210874) were used to manipulate NRDE2 expression in human cells, enabling the authors to investigate NRDE2's essential role in RNA splicing and genome stability.
Showing 1-1 of 1 papers.
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