SRGAP2 (NM_015326) Human Tagged ORF Clone

SKU RC224139

SRGAP2 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SRGAP2
• Synonyms: ARHGAP34; FNBP2; SRGAP2A; SRGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224139 representing NM_015326; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGCAGAGTACGATACTCAGGTCAAAGAGA; TCCGTGCTCAGCTCACAGAGCAGATGAAATGCCTGGACCAGCAGTGTGAGCTTCGGGTGCAACTGTTGCA; GGACCTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAGATGGACTACTCCCGCAACCTGGAGAAGCTG; GCAGAACGCTTCCTGGCCAAGACACGCAGCACCAAGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCT; CTCCAGTCAACTGCTGGAATCTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAG; TGACATCTACCTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA; AAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTCTACTCGGTGA; TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAAAGGAGGCGGAGAAGCA; GGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGACCGGCAGACCCCACGCTCCCCTGACTCCACG; GCCAACGTTCGCATTGAGGAGAAACATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA; AGCGTCAAGCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTACTTGCTGGCTTT; GGAGGCAACCAATGCATCTGTCTTCAAGTACTACATCCATGACCTATCTGACCTTATTGATCAGTGTTGT; GACTTAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTAAACCTGGAAC; AGTCGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACCTGGATGCCACCAGTGACAAGCA; GCGCCTCATGGAGATGTACAACAACGTCTTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCACATGGGG; GATATGGCTTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTACAGAGATGCCAACAACTGC; AGTCTCGCTTATCCACTCTAAAGATTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAAC; CATCCAGGACATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGGAG; TCCGTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCCAACCAGC; AAGAGACAGAGCAGTTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGCAGGAACCTCATCACCAAGTT; ACAAGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGATTGCAGTCTAGCCAGG; CGCAGCTCAACTGTGAGGAAACAGGACTCCAGCCAGGCAATTCCTCTGGTGGTGGAAAGCTGTATCCGGT; TTATCAGCAGACACGGACTACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAATGA; CATCAAAAATGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAACGACCATGACATGGATTCC; ATAGCTGGTGTCCTGAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCTTTCATG; ACCTGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCACATCCGGAAAGTCCTCCTAGT; CCTGCCCAAAACCACTCTGATTATCATGAGATACCTCTTTGCCTTCCTCAATCATTTATCACAGTTCAGT; GAAGAGAACATGATGGACCCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGAGG; GCCACGACCAGGTGTCCTGCCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAGCATGAGAA; CATCTTCCCAAGCCCCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT; GATAGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAGAGCACCACACGA; GCGATGACGAATGTGAGCCCATCGAGGCCATTGCCAAGTTTGACTACGTGGGCCGGACAGCCCGAGAGCT; GTCCTTTAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGGGCTTCCGACGACTGGTGGGAAGGCCGGCAC; AATGGCATCGACGGACTCATCCCCCATCAGTACATCGTGGTCCAAGACACCGAGGACGGTGTCGTGGAGA; GGTCCAGCCCCAAGTCTGAGATTGAGGTCATTTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAGCGGG; GGCCAGCTGTCCCAGTGGGGGTCATGTAGCCGATATTTATCTTGCAAACATCAACAAGCAAAGGAAGCGT; CCAGAATCTGGGAGCATCCGGAAAACTTTTCGGAGTGACAGCCATGGGCTGAGCAGTTCCCTGACTGACT; CCTCCTCCCCAGGGGTGGGGGCTAGCTGCCGCCCATCCTCCCAGCCCATCATGAGCCAGAGCCTCCCCAA; AGAAGGGCCAGATAAGTGTTCCATCAGTGGGCACGGGAGCCTCAACTCCATCAGCCGCCACTCATCCCTG; AAGAATCGGCTGGATAGTCCACAGATCCGGAAGACTGCCACAGCGGGAAGGTCAAAAAGCTTCAATAACC; ATCGGCCCATGGACCCTGAGGTCATTGCTCAGGATATTGAGGCAACAATGAACTCGGCCCTGAATGAGCT; ACGGGAACTAGAACGGCAGAGCAGTGTCAAACACACCCCTGACGTGGTTCTGGACACCTTGGAGCCCCTC; AAAACCTCCCCAGTGGTGGCCCCCACGTCAGAGCCCTCCAGCCCTCTGCACACCCAGCTCCTCAAGGACC; CCGAGCCCGCCTTCCAGCGCAGCGCCAGTACTGCTGGGGACATCGCCTGCGCCTTCCGGCCTGTCAAGTC; TGTCAAGATGGCTGCCCCGGTCAAACCACCAGCCACACGGCCCAAGCCCACTGTCTTCCCCAAAACAAAT; GCCACTAGCCCTGGTGTCAACTCATCAACTTCCCCACAGTCTACTGACAAGTCTTGTACTGTC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224139 representing NM_015326; Red=Cloning site Green=Tags(s); ; MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL; AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK; KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST; ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCC; DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG; DMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME; SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR; RSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS; IAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFS; EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYC; DSPHGETTSVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH; NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINKQRKR; PESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISGHGSLNSISRHSSL; KNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELRELERQSSVKHTPDVVLDTLEPL; KTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTN; ATSPGVNSSTSPQSTDKSCTV; ; SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015326
• ORF Size: 3213 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015326.2
• RefSeq Size: 6305 bp
• RefSeq ORF: 3216 bp
• Locus ID: 23380
• UniProt ID: O75044
• Cytogenetics: 1q32.1
• Protein Pathways: Axon guidance
• MW: 120.7 kDa
• Summary: This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014]