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UNC45B (NM_173167) Human Tagged ORF Clone

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SKU
RC223751
UNC45B (Myc-DDK-tagged)-Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UNC45B
Synonyms CMYA4; CTRCT43; MFM11; SMUNC45; UNC-45B; UNC45
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223751 representing NM_173167
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGTGGAAGCGGTACAGCTGAAGGAGGAAGGAAACCGGCATTTCCAGCTCCAGGACTACAAGG
CCGCCACAAATAGCTACAGCCAGGCCCTGAAGCTGACCAAGGACAAGGCCCTGCTGGCCACGCTTTATCG
GAACCGGGCAGCCTGTGGCCTGAAAACGGAGAGCTACATCCAGGCAGCTTCAGATGCCTCCAGAGCCATC
GACATCAACTCCTCGGACATCAAGGCTCTGTATCGGCGATGCCAGGCACTGGAGCACCTGGGGAAGCTGG
ACCAGGCCTTCAAAGACGTGCAGCGTTGTGCCACCCTCGAGCCACGGAACCAGAACTTCCAGGAGATGCT
GAGGAGACTCAACACCAGCATTCAGGAGAAGCTCCGAGTGCAGTTCTCCACAGACTCGAGGGTACAGAAG
ATGTTTGAGATCCTCTTGGATGAAAACAGTGAGGCTGATAAGCGGGAAAAGGCTGCCAACAATCTCATTG
TCCTAGGCCGTGAGGAAGCAGGGGCTGAGAAGATCTTCCAGAACAATGGAGTAGCCTTGCTACTGCAGCT
TCTGGACACTAAGAAGCCTGAGCTGGTGCTGGCTGCAGTGCGGACCCTGTCGGGCATGTGCAGCGGCCAC
CAAGCCAGAGCCACAGTGATTCTGCATGCAGTGCGGATAGACCGAATCTGTAGCCTCATGGCCGTGGAGA
ATGAGGAGATGTCTCTGGCTGTCTGCAACCTGCTCCAAGCCATCATTGACTCCTTGTCTGGGGAGGACAA
GCGGGAGCATCGAGGGAAGGAGGAGGCCCTGGTTCTAGACACCAAGAAGGACCTGAAGCAGATCACCAGC
CACCTGCTGGACATGCTAGTCAGCAAGAAGGTGTCTGGCCAGGGCAGGGATCAGGCGCTGAACCTGCTCA
ATAAGAATGTTCCCAGGAAGGACCTTGCCATTCATGACAACTCACGTACCATCTATGTGGTGGATAATGG
TCTGAGGAAGATCCTGAAGGTTGTGGGGCAGGTTCCAGATCTGCCATCCTGCCTGCCCCTGACTGACAAC
ACCCGCATGCTGGCCTCTATCCTCATCAACAAGCTCTATGATGACCTGCGCTGTGACCCGGAGCGCGATC
ACTTCCGCAAGATCTGTGAGGAATATATCACGGGCAAGTTTGACCCCCAGGACATGGACAAGAACTTGAA
TGCCATCCAGACAGTGTCAGGGATCCTGCAGGGCCCCTTTGACCTGGGCAACCAGCTGCTGGGACTGAAA
GGTGTGATGGAGATGATGGTGGCACTATGTGGCTCAGAGCGCGAGACGGACCAGCTGGTGGCCGTGGAGG
CCCTCATCCATGCCTCCACGAAGCTCAGCCGCGCCACCTTCATCATCACCAATGGAGTGTCACTGCTCAA
ACAGATCTACAAGACCACCAAAAATGAGAAGATCAAGATCCGCACACTGGTGGGACTCTGTAAGCTCGGC
TCTGCAGGTGGCACAGACTACGGTCTCAGGCAGTTTGCGGAAGGGTCGACAGAAAAACTGGCCAAACAGT
GTCGCAAGTGGCTGTGCAATATGTCCATAGACACTCGGACCCGACGCTGGGCAGTGGAGGGCCTGGCCTA
CCTCACGCTGGACGCTGATGTGAAGGACGACTTTGTCCAGGACGTCCCTGCCCTGCAGGCCATGTTTGAG
CTGGCCAAG::::::ACCAGTGACAAGACCATCCTGTACTCGGTGGCCACCACCCTGGTGAACTGCACCA
ACAGCTACGATGTCAAGGAGGTCATCCCAGAGCTTGTCCAGCTCGCCAAGTTCTCCAAGCAGCATGTGCC
CGAGGAACACCCCAAGGACAAGAAGGACTTTATAGACATGCGGGTGAAGCGGCTTCTGAAGGCGGGTGTC
ATCTCTGCCCTGGCTTGCATGGTGAAAGCAGATAGTGCCATCCTCACTGACCAGACCAAGGAGCTGCTGG
CCAGGGTATTCCTGGCACTGTGTGACAACCCAAAGGACCGAGGCACCATTGTGGCTCAAGGTGGTGGCAA
GGCCCTGATTCCCCTGGCTTTGGAGGGCACAGATGTGGGCAAGGTGAAGGCAGCCCACGCTCTAGCAAAG
ATCGCTGCTGTCTCCAATCCGGACATTGCTTTTCCTGGGGAGCGGGTGTATGAGGTGGTGCGGCCCCTTG
TAAGACTCTTGGACACACAGAGGGATGGGCTTCAGAACTATGAGGCTCTCCTAGGCCTCACCAACCTGTC
TGGGCGGAGTGACAAACTCCGGCAGAAGATCTTTAAGGAGAGGGCCTTGCCAGACATCGAGAACTACATG
TTTGAGAATCATGATCAGCTGCGGCAGGCGGCCACCGAGTGCATGTGCAACATGGTGCTCCACAAGGAGG
TACAGGAAAGGTTCTTGGCTGACGGGAATGACCGGCTGAAGCTGGTGGTGCTGCTCTGCGGGGAGGATGA
TGATAAGGTGCAGAATGCGGCTGCAGGGGCTCTGGCCATGCTGACAGCAGCACACAAGAAACTGTGCCTC
AAGATGACTCAAGTGACAACCCAGTGGTTGGAGATCCTCCAGCGGCTTTGCCTGCACGACCAGCTGTCTG
TCCAACACCGGGGCCTGGTCATTGCCTACAACCTACTGGCAGCCGATGCTGAGCTGGCCAAGAAGCTGGT
GGAGAGTGAGCTGCTGGAGATCCTGACTGTGGTGGGCAAACAGGAGCCAGATGAGAAGAAGGCAGAAGTG
GTTCAGACAGCCCGAGAATGTCTCATCAAGTGCATGGATTATGGTTTCATTAAACCAGTGTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC223751 representing NM_173167
Red=Cloning site Green=Tags(s)

MAEVEAVQLKEEGNRHFQLQDYKAATNSYSQALKLTKDKALLATLYRNRAACGLKTESYIQAASDASRAI
DINSSDIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQEMLRRLNTSIQEKLRVQFSTDSRVQK
MFEILLDENSEADKREKAANNLIVLGREEAGAEKIFQNNGVALLLQLLDTKKPELVLAAVRTLSGMCSGH
QARATVILHAVRIDRICSLMAVENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLKQITS
HLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLAIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDN
TRMLASILINKLYDDLRCDPERDHFRKICEEYITGKFDPQDMDKNLNAIQTVSGILQGPFDLGNQLLGLK
GVMEMMVALCGSERETDQLVAVEALIHASTKLSRATFIITNGVSLLKQIYKTTKNEKIKIRTLVGLCKLG
SAGGTDYGLRQFAEGSTEKLAKQCRKWLCNMSIDTRTRRWAVEGLAYLTLDADVKDDFVQDVPALQAMFE
LAKXXTSDKTILYSVATTLVNCTNSYDVKEVIPELVQLAKFSKQHVPEEHPKDKKDFIDMRVKRLLKAGV
ISALACMVKADSAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLALEGTDVGKVKAAHALAK
IAAVSNPDIAFPGERVYEVVRPLVRLLDTQRDGLQNYEALLGLTNLSGRSDKLRQKIFKERALPDIENYM
FENHDQLRQAATECMCNMVLHKEVQERFLADGNDRLKLVVLLCGEDDDKVQNAAAGALAMLTAAHKKLCL
KMTQVTTQWLEILQRLCLHDQLSVQHRGLVIAYNLLAADAELAKKLVESELLEILTVVGKQEPDEKKAEV
VQTARECLIKCMDYGFIKPVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173167
ORF Size 2793 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173167.3
RefSeq Size 5694 bp
RefSeq ORF 2796 bp
Locus ID 146862
UniProt ID Q8IWX7
Cytogenetics 17q12
MW 103.6 kDa
Summary This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_173167" in other vectors (4)
SKU Description Size Price
RC223751L3 Lenti ORF clone of Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC223751L4 Lenti ORF clone of Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1, mGFP tagged 10 ug
$600.00
RG223751 UNC45B (tGFP-tagged) - Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1 10 ug
$789.00
SC306792 UNC45B (untagged)-Human unc-45 homolog B (C. elegans) (UNC45B), transcript variant 1 10 ug
$853.00

Plasmid Map for RC223751

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC223751
Circular map for RC223751

file-alt Citations

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