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XPF (ERCC4) (NM_005236) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC223300L1
Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,139.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol XPF
Synonyms ERCC11; FANCQ; RAD1; XFEPS; XPF
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC223300).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005236
ORF Size 2748 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005236.1
RefSeq Size 2751 bp
RefSeq ORF 2751 bp
Locus ID 2072
UniProt ID Q92889
Cytogenetics 16p13.12
Protein Families Druggable Genome
Protein Pathways Nucleotide excision repair
MW 104.3 kDa
Summary The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).provided by RefSeq, Mar 2009
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"NM_005236" in other vectors (6)
SKU Description Size Price
RC223300 ERCC4 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) 10 ug
$839.00
RC223300L2 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged 10 ug
$1,139.00
RC223300L3 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged 10 ug
$1,139.00
RC223300L4 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged 10 ug
$1,139.00
RG223300 ERCC4 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) 10 ug
$789.00 $1,039.00
SC314914 ERCC4 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) 10 ug
$840.00

Plasmid Map for RC223300L1

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Cloning scheme for RC223300L1
Circular map for RC223300L1

file-alt Citations

A Unique Subset of Epithelial Ovarian Cancers with Platinum Sensitivity and PARP Inhibitor Resistance
Ceccaldi, O'Connor, Mouw et al
Cancer Res. (2015) 75 (4), 628-34 DOI: 10.1158/0008-5472.CAN-14-2593
AI Summary: In this study, OriGene’s pLenti vector was used to complement ERCC4-deficient cell lines with wild-type or mutant ERCC4, enabling functional analysis of NER gene alterations in ovarian cancer platinum sensitivity and PARP inhibitor resistance.
Snippet: ERCC6-deficient line was complemented with either wild-type or mutant N-terminal Flag-tagged ERCC6, cloned in a pOZ vector. The ERCC4-deficient line was complemented with C-terminal Myc-tagged wild-type or mutant ERCC4, cloned in a pLenti vector (Origene ... Cite SaveSaving
Showing 1-1 of 1 papers.
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