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XPF (ERCC4) (NM_005236) Human Untagged Clone

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SKU
SC314914
ERCC4 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4)
  $840.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol XPF
Synonyms ERCC11; FANCQ; RAD1; XFEPS; XPF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_005236 edited
ATGGAGTCAGGGCAGCCGGCTCGACGGATTGCCATGGCGCCGCTGCTGGAGTACGAGCGA
CAGCTGGTGCTGGAACTGCTCGACACTGACGGGCTAGTAGTGTGCGCCCGCGGGCTCGGC
GCGGACCGGCTCCTCTACCACTTTCTCCAGCTGCACTGCCACCCAGCCTGCCTGGTGCTG
GTGCTCAACACGCAGCCGGCCGAGGAGGAGTATTTTATCAATCAGCTGAAGATAGAAGGA
GTTGAACACCTCCCTCGCCGTGTAACAAATGAAATCACAAGCAACAGTCGCTATGAAGTT
TACACACAAGGTGGTGTTATATTTGCGACAAGTAGGATACTTGTGGTTGACTTCTTGACT
GATAGAATACCTTCAGATTTAATTACTGGCATCTTGGTGTATAGAGCCCACAGAATAATC
GAGTCTTGTCAAGAAGCATTCATCTTGCGCCTCTTTCGCCAGAAAAACAAACGTGGTTTT
ATTAAAGCTTTCACAGACAATGCTGTTGCCTTTGATACTGGTTTTTGTCATGTGGAAAGA
GTGATGAGAAATCTTTTTGTGAGGAAACTGTATCTGTGGCCAAGGTTCCATGTAGCAGTA
AACTCATTTTTAGAACAGCACAAACCTGAAGTTGTAGAAATCCATGTTTCTATGACACCT
ACCATGCTTGCTATACAGACTGCTATACTGGACATTTTAAATGCATGTCTAAAGGAACTA
AAATGCCATAACCCATCGCTTGAAGTGGAAGATTTATCTTTAGAAAATGCTATTGGAAAA
CCTTTTGACAAGACAATCCGCCATTATCTGGATCCTTTGTGGCACCAGCTTGGAGCCAAG
ACTAAATCCTTAGTTCAGGATTTGAAGATATTACGAACTTTGCTGCAGTATCTCTCTCAG
TATGATTGTGTCACATTTCTTAATCTTCTGGAATCTCTGAGAGCAACGGAAAAAGCTTTT
GGTCAGAATTCAGGTTGGCTGTTTCTTGACTCCAGCACCTCGATGTTTATAAATGCTCGA
GCAAGGGTTTATCATCTTCCAGATGCCAAAATGAGTAAAAAAGAAAAAATATCTGAAAAA
ATGGAAATTAAAGAAGGGGAAGAAACAAAAAAGGAACTGGTCCTAGAAAGCAACCCAAAG
TGGGAGGCACTGACTGAAGTATTAAAAGAAATTGAGGCAGAAAATAAGGAGAGTGAAGCT
CTTGGTGGTCCAGGTCAAGTACTGATTTGTGCAAGTGATGACCGAACATGTTCCCAGCTG
AGAGACTATATCACTCTTGGAGCGGAGGCCTTCTTATTGAGGCTCTACAGGAAAACCTTT
GAGAAGGATAGCAAAGCTGAAGAAGTCTGGATGAAATTTAGGAAGGAAGACAGTTCAAAG
AGAATTAGGAAATCTCACAAAAGACCTAAAGACCCCCAAAACAAAGAACGGGCTTCTACC
AAAGAAAGAACCCTCAAAAAGAAAAAACGGAAGTTGACCTTAACTCAAATGGTAGGAAAA
CCTGAAGAACTGGAAGAGGAAGGAGATGTCGAGGAAGGATATCGTCGAGAAATAAGCAGT
AGCCCAGAAAGCTGCCCGGAAGAAATTAAGCATGAAGAATTTGATGTAAATTTGTCATCG
GATGCTGCTTTCGGAATCCTGAAAGAACCCCTCACTATCATCCATCCGCTTCTGGGTTGC
AGCGACCCCTATGCTCTGACAAGGGTACTACATGAAGTGGAGCCAAGATACGTGGTTCTT
TATGACGCAGAGCTAACCTTTGTTCGGCAGCTTGAAATTTACAGGGCGAGTAGGCCTGGG
AAACCTCTGAGGGTTTACTTTCTTATATACGGAGGTTCAACTGAGGAACAACGCTATCTC
ACTGCTTTGCGGAAAGAAAAGGAAGCTTTTGAAAAACTCATAAGGGAAAAAGCAAGCATG
GTTGTCCCTGAAGAAAGAGAAGGCAGAGATGAAACAAACTTAGACCTAGTAAGAGGCACA
GCATCTGCAGATGTTTCCACTGACACTCGGAAAGCCGGTGGCCAGGAACAGAATGGTACA
CAGCAAAGCATAGTTGTGGATATGCGTGAATTTCGAAGTGAGCTTCCATCTCTGATCCAT
CGTCGGGGCATTGACATTGAACCCGTGACTTTAGAGGTTGGAGATTACATCCTCACTCCA
GAAATGTGCGTGGAGCGCAAGAGTATCAGTGATTTAATCGGCTCTTTAAATAACGGCCGC
CTCTACAGCCAGTGCATCTCCATGTCCCGCTACTACAAGCGTCCCGTGCTTCTGATTGAG
TTTGACCCTAGCAAGCCTTTCTCTCTCACTTCCCGAGGTGCCTTGTTTCAGGAGATCTCC
AGCAATGACATTAGTTCCAAACTCACTCTTCTTACACTTCACTTCCCCAGACTACGGATT
CTCTGGTGCCCCTCTCCTCATGCAACGGCGGAGTTGTTTGAGGAGCTGAAACAAAGCAAG
CCACAGCCTGATGCGGCGACAGCACTGGCCATTACAGCAGATTCCGAAACCCTTCCCGAG
TCAGAGAAGTATAATCCTGGTCCCCAAGACTTCTTGTTAAAAATGCCAGGGGTGAATGCC
AAAAACTGCCGCTCCTTGATGCACCACGTTAAGAACATCGCAGAATTAGCAGCCCTGTCA
CAAGACGAGCTCACGAGTATTCTGGGGAATGCTGCAAATGCCAAACAGCTTTATGATTTC
ATTCACACCTCTTTTGCAGAAGTCGTATCAAAAGGAAAAGGGAAAAAGTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005236 unedited
CTCCACAGAGCACAACGACATACCACTAGGGCGGCCGCGAACTCGGCACGAGACGGAGCC
AGGGCAGCCGGCCCGACGGATCGCCACGGCGCCGCCGCCGGAGCACGAGCGACAGCCGGT
GCTGGAACCGCTCGACACTGACGGGCTAGTAGCGTGCGCCCGCGGGCTCGGCGCGGACCG
GCCCCTCTACCACTACCTCCAGCCGCACTGCCACCCAGCCTGCCTGGTGCTGGTGCTCAA
CACGCAGCCGGCCGAGGAGGAGTATTTTATCAATCAGCTGAAGATAGAAGGAGTTGAACA
CCTCCCTCGCCGTGTAACAAATGAAATCACAAGCAACAGTCGCTATGAAGTCTACACACA
AGGTGGTGTTATATTTGCGACAAGTAGGATACTTGTGGTTGACTTCTTGACTGATAGAAT
ACCTTCAGATTTAATTACTGGCATCTTGGTGTATAGAGCCCACAGAATAATCGAGTCTTG
TCAAGAAGCATTCATCTTGCGCCTCTTTCGCCAGAAAAACAAACGTGGTTTTATTAAAGC
TTTCACAGACAATGCTGTTGCCTTTGATACTGGTTTTTGTCATGTGGAAAGAGTGATGAG
AAATCTTTTTGTGAGGAAACTGTATCTGTGGCCAAGGTTCCATGTAGCAGTAAACTCATT
TTTAGAACAGCACAAACCTGAAGTTGTAGAAATCCATGTTTCTATGACACCTACCATGCT
TGCTATACAGACTGCTATACTGGACATTTTAAATGCATGTCTAAAGGAACTAAAATGCCA
TAACCCATCGCTTGAAGTGGAGATTTATCTTTAGAAAATGCTATTGGAAAACCTTTTGAC
AGACATCCGCCATTATTCTGGATCCTTTGTGCACAGCTTGAGCCCAGACTAATCCTAGTC
AGGATTGGAGATTTACGACTTGCTGCAGTA
Restriction Sites Please inquire |
ACCN NM_005236
Insert Size 5500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005236.1, NP_005227.1
RefSeq Size 2751 bp
RefSeq ORF 2751 bp
Locus ID 2072
UniProt ID Q92889
Cytogenetics 16p13.12
Protein Families Druggable Genome
Protein Pathways Nucleotide excision repair
Summary The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).provided by RefSeq, Mar 2009
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Documents
Resources
"NM_005236" in other vectors (6)
SKU Description Size Price
RC223300 ERCC4 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) 10 ug
$839.00
RC223300L1 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged 10 ug
$1,139.00
RC223300L2 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged 10 ug
$1,139.00
RC223300L3 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), Myc-DDK-tagged 10 ug
$1,139.00
RC223300L4 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4), mGFP tagged 10 ug
$1,139.00
RG223300 ERCC4 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 4 (ERCC4) 10 ug
$789.00 $1,039.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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