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DUS4L (NM_181581) Human Tagged ORF Clone

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SKU
RC222488
DUS4L (Myc-DDK-tagged)-Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUS4L
Synonyms DUS4; PP35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222488 representing NM_181581
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGTGACTGCATGCAAACGACAATATGTCAGGAAAGAAAAAAAGATCCCATAGAAATGTTTCATT
CTGGACAGCTGGTAAAAGTCTGTGCCCCAATGGTTCGATATTCAAAGTTGGCTTTTAGGACACTAGTAAG
AAAATATAGTTGTGATCTGTGTTACACACCAATGATTGTTGCCGCTGATTTTGTCAAATCTATAAAAGCC
AGAGACAGCGAATTTACCACAAATCAAGGTGATTGCCCATTGATTGTTCAGTTTGCTGCTAACGATGCAA
GACTTTTATCTGATGCTGCTCGTATAGTCTGTCCTTATGCGAATGGAATAGACATTAACTGTGGTTGCCC
TCAGAGGTGGGCAATGGCAGAAGGTTATGGGGCTTGCTTAATAAACAAGCCAGAGCTTGTTCAAGACATG
GTGAAACAAGTAAGAAATCAAGTGGAAACCCCTGGATTTTCAGTTTCTATTAAAATAAGGATCCATGATG
ACCTTAAAAGAACTGTAGATCTTTGTCAAAAGGCTGAAGCAACAGGAGTTTCATGGATTACAGTCCATGG
AAGAACTGCTGAAGAAAGACATCAGCCAGTGCACTATGATTCCATTAAAATAATTAAGGAAAATATGTCT
ATACCTGTAATTGCTAATGGAGACATCAGAAGCTTAAAGGAAGCAGAAAATGTGTGGCGGATTACTGGGA
CAGATGGTGTGATGGTTGCAAGAGGACTCTTAGCAAACCCGGCCATGTTTGCTGGATATGAGGAAACCCC
ACTGAAATGCATCTGGGACTGGGTTGACATTGCTCTTGAACTCGGGACTCCTTACATGTGTTTCCATCAA
CATTTAATGTACATGATGGAAAAGATAACTTCAAGGCAGGAAAAAAGGGTATTTAATGCTCTGTCAAGCA
CATCAGCAATCATAGATTACCTTACAGACCATTATGGCATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222488 representing NM_181581
Red=Cloning site Green=Tags(s)

MKSDCMQTTICQERKKDPIEMFHSGQLVKVCAPMVRYSKLAFRTLVRKYSCDLCYTPMIVAADFVKSIKA
RDSEFTTNQGDCPLIVQFAANDARLLSDAARIVCPYANGIDINCGCPQRWAMAEGYGACLINKPELVQDM
VKQVRNQVETPGFSVSIKIRIHDDLKRTVDLCQKAEATGVSWITVHGRTAEERHQPVHYDSIKIIKENMS
IPVIANGDIRSLKEAENVWRITGTDGVMVARGLLANPAMFAGYEETPLKCIWDWVDIALELGTPYMCFHQ
HLMYMMEKITSRQEKRVFNALSSTSAIIDYLTDHYGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181581
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181581.3
RefSeq Size 2252 bp
RefSeq ORF 954 bp
Locus ID 11062
UniProt ID O95620, A4D0R5
Cytogenetics 7q22.3
MW 35.6 kDa
Summary Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.UniProtKB/Swiss-Prot Function
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"NM_181581" in other vectors (4)
SKU Description Size Price
RC222488L3 Lenti ORF clone of Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L), Myc-DDK-tagged 10 ug
$600.00
RC222488L4 Lenti ORF clone of Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L), mGFP tagged 10 ug
$600.00
RG222488 DUS4L (tGFP-tagged) - Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L) 10 ug
$489.00 $500.00
SC111180 DUS4L (untagged)-Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L) 10 ug
$300.00

Plasmid Map for RC222488

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC222488
Circular map for RC222488

file-alt Citations

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