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DUS4L (NM_181581) Human Untagged Clone

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SKU
SC111180
DUS4L (untagged)-Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DUS4L
Synonyms DUS4; PP35
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111180 sequence for NM_181581 edited (data generated by NextGen Sequencing)
ATGAAGAGTGACTGCATGCAAACGACAATATGTCAGGAAAGAAAAAAAGATCCCATAGAA
ATGTTTCATTCTGGACAGCTGGTAAAAGTCTGTGCCCCAATGGTTCGATATTCAAAGTTG
GCTTTTAGGACACTAGTAAGAAAATATAGTTGTGATCTGTGTTACACACCAATGATTGTT
GCCGCTGATTTTGTCAAATCTATAAAAGCCAGAGACAGCGAATTTACCACAAATCAAGGT
GATTGCCCATTGATTGTTCAGTTTGCTGCTAACGATGCAAGACTTTTATCTGATGCTGCT
CGTATAGTCTGTCCTTATGCGAATGGAATAGACATTAACTGTGGTTGCCCTCAGAGGTGG
GCAATGGCAGAAGGTTATGGGGCTTGCTTAATAAACAAGCCAGAGCTTGTTCAAGACATG
GTGAAACAAGTAAGAAATCAAGTGGAAACCCCTGGATTTTCAGTTTCTATTAAAATAAGG
ATCCATGATGACCTTAAAAGAACTGTAGATCTTTGTCAAAAGGCTGAAGCAACAGGAGTT
TCATGGATTACAGTCCATGGAAGAACTGCTGAAGAAAGACATCAGCCAGTGCACTATGAT
TCCATTAAAATAATTAAGGAAAATATGTCTATACCTGTAATTGCTAATGGAGACATCAGA
AGCTTAAAGGAAGCAGAAAATGTGTGGCGGATTACTGGGACAGATGGTGTGATGGTTGCA
AGAGGACTCTTAGCAAACCCGGCCATGTTTGCTGGATATGAGGAAACCCCACTGAAATGC
ATCTGGGACTGGGTTGACATTGCTCTTGAACTCGGGACTCCTTACATGTGTTTCCATCAA
CATTTAATGTACATGATGGAAAAGATAACTTCAAGGCAGGAAAAAAGGGTATTTAATGCT
CTGTCAAGCACATCAGCAATCATAGATTACCTTACAGACCATTATGGCATTTGA

Clone variation with respect to NM_181581.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_181581 unedited
GGAGAGGAAACCCCCGTCCTAGNTAGGCGGAAACGCCATTCGGCACCANACCTGGCGAAC
TGACTCTCAGCCCGCGCCTGGGCTAAGCCTGGCTAGGAGCCGCGCAGGTACTCGAGCAGT
GGGCGCCCAGGGTCCGAGTGCTCTGCGCCCATCGCACCGAGGGATCCAAGGCCGTCGGGC
CGGCGCTTTCAGCTGTCTCTCGCAGCATTTCACTGCCTCGCCCCCTGGGCTGGCGTCGTG
CCCTATCCAAGGAGAAGTTTCAGTCACGTGCCAGACTTTTTCTTAATTACAAACTGATAG
ATGGTCCACATCTCAACTAACAGTATTGACGCTGTAAGAGCATCAGATTTGAAACATATC
TGTATTAATGAAGAGTGACTGCATGCAAACGACAATATGTCAGGAAAGAAAAAAAGATCC
CATAGAAATGTTTCATTCTGGACAGCTGGTAAAAGTCTGTGCCCCAATGGTTCGATATTC
AAAGTTGGCTTTTAAGACACTAGTAAGAAAATATAGTTGTGATCTGTGTTACACACCAAT
GATTGTTGCCGCTGATTTTGTCAAATCTATAAAAGCCAGAGACAGCGAATTTACCACAAA
TCAAGGTGATTGCCCATTGATTGTTCAGTTTGCTGCTAACGATGCAAGACTCTTATCTGA
TGCTGCTCGTATAGTCTGTCCTTATGCGAATGGGATAGACATTAACTGTGGTTGCCCTCA
TATGTGGGNCATGGCANAATGTTATGGGAGCTAGCTTAANTAACAAGCCAGAGCTTGGCT
CAGACATGCTGAATCAAGCTAGATATCAAGTGGGAACCCCTGGNATTTCAGCTTCTATTA
AAATAGGATCCATGATGACCTTTAAAGACTGTAGATCTTTGTCANAAGGCTGATGCACAG
AGTCTCATGGATTACAGNCCATGGNAGAACTGGCTGAGAATGACTCAGCCAGGGCCTATG
ATCCATTNAAATATNAGGNAATATGNCCTACCTGGNATGCTATGGAGACTCANAGCTTAT
GAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_181581 unedited
GTCCATTATGNACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTATGTTAAA
ATGGATTCATTCAATATCTCCCAAACGTGAATTAAACAGTTTTACATAATTGTTTCCTTA
TAACAGTTTTCATGTTGGCAAAACAAAATAATACTTTAATTATGAACGGTTTTATTTCAG
TCTGAGATACTTAATTCTTGCAGTGCATGCCCACCGATAACAAAATCTATTAATAACACT
CCAAAACCATGGGACTTTAAAGTTACAATCAACGGCTGAGAGGCACAAAAGTAATTAGGT
TTTAAAGTTAAAAACAAAACCTGGTTACTATTCTCTAGGAAATATTTCAAATATATAATC
ATCTCCAGGTTGGACACCAAATTTCCTATACTTAACATATGGTAGATTGCAATTAATTTT
ATCTTTTTAGGTAGGAGATTATTAAGCTTTCAATCTTTGGAGGACCTAGAAGTTTTGAAA
TGTACCAAATTTAATGTCATTAACCAAATGGAATTTTTAATATAAGTTCTGAGTGCAATT
CTATTCAGAATTTCCTGTTCTTTTTCATTTTGGATGGATGTTGGTATTTGTTCCACCACT
TTTTTGCACCTGCATACTTCTTGTTAATTTATAGATACTATAAGCCATAATTTCTTAGTA
TTAAATTTTATTTTANAATGTTAAAAACATGATTAGGAAAAAATCCCACTCTACCCATTC
CCCCAATATCTATAGAAACAGATTCANAGCAGTATTTGTCAATGGTTGCCTAAGATGATC
AAGATGTTTGAACCACTGGGAGTTTTCTTTAAACTGTGTTTTTCGGGGTCTACTCCAGAT
CTACCTATCAGGATCTCTTGAGTGGGGGCCTACAACTGATT
Restriction Sites NotI-NotI |
ACCN NM_181581
Insert Size 2400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181581.1, NP_853559.1
RefSeq Size 2252 bp
RefSeq ORF 954 bp
Locus ID 11062
UniProt ID O95620
Cytogenetics 7q22.3
Summary Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) is protein-coding. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_181581" in other vectors (4)
SKU Description Size Price
RC222488 DUS4L (Myc-DDK-tagged)-Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L) 10 ug
$300.00
RC222488L3 Lenti ORF clone of Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L), Myc-DDK-tagged 10 ug
$600.00
RC222488L4 Lenti ORF clone of Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L), mGFP tagged 10 ug
$600.00
RG222488 DUS4L (tGFP-tagged) - Human dihydrouridine synthase 4-like (S. cerevisiae) (DUS4L) 10 ug
$489.00 $500.00

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