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MYLK3 (NM_182493) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC222246L1
Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3)
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,050.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MYLK3
Synonyms caMLCK; MLCK; MLCK2
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC222246).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182493
ORF Size 2457 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182493.2
RefSeq Size 7998 bp
RefSeq ORF 2460 bp
Locus ID 91807
UniProt ID Q32MK0
Cytogenetics 16q11.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
MW 88.8 kDa
Summary Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 PubMed 18202317).supplied by OMIM, Jul 2008
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"NM_182493" in other vectors (8)
SKU Description Size Price
RC222246 MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$589.00 $750.00
RC222246L2 Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RC222246L3 Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RC222246L4 Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RG222246 MYLK3 (tGFP-tagged) - Human myosin light chain kinase 3 (MYLK3) 10 ug
$789.00 $950.00
SC307402 MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$802.00
SC318299 MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$826.00
SC323576 MYLK3 (untagged)-Kinase deficient mutant (K520M) of Human myosin light chain kinase 3 (MYLK3) 10 ug
$729.00

Plasmid Map for RC222246L1

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Cloning scheme for RC222246L1
Circular map for RC222246L1

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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