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MYLK3 (NM_182493) Human Untagged Clone

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SKU
SC318299
MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3)
  $826.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MYLK3
Synonyms caMLCK; MLCK; MLCK2
Vector pCMV6 series
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_182493, the custom clone sequence may differ by one or more nucleotides
ATGTCAGGAACCTCCAAGGAGAGTCTGGGGCATGGGGGGCTGCCAGGGTTGGGCAAGACC
TGCTTAACAACCATGGACACAAAGCTGAACATGCTGAACGAGAAGGTGGACCAGCTCCTG
CACTTCCAAGAAGATGTCACAGAGAAGTTGCAGAGCATGTGCCGAGACATGGGCCACCTG
GAGCGGGGCCTGCACAGGCTGGAGGCCTCCCGGGCACCGGGCCCGGGCGGGGCTGATGGG
GTTCCCCACATTGACACCCAGGCTGGGTGGCCCGAGGTCCTGGAGCTGGTGAGGGCCATG
CAGCAGGATGCGGCCCAGCACGGTGCCAGGCTGGAGGCCCTCTTCAGGATGGTGGCTGCG
GTGGACAGGGCCATCGCTTTGGTGGGGGCCACGTTCCAGAAATCAAAGGTGGCGGATTTC
CTCATGCAGGGGCGTGTGCCCTGGAGGAGAGGCAGCCCAGGTGACAGCCCTGAGGAGAAT
AAAGAGCGAGTGGAAGAAGAGGGAGGAAAACCAAAGCATGTGCTGAGCACCAGTGGGGTG
CAGTCTGATGCCAGGGAGCCTGGGGAAGAGAGCCAGAAGGCGGACGTGCTGGAGGGGACA
GCGGAGAGGCTGCCCCCCATCAGAGCGTCAGGGCTGGGAGCTGACCCCGCCCAGGCAGTG
GTCTCACCGGGCCAGGGAGATGGTGTTCCTGGCCCAGCCCAGGCATTCCCTGGCCACCTG
CCCCTGCCCACAAAGGTGGAAGCCAAGGCTCCTGAGACACCCAGCGAGAACCTCAGGACT
GGCCTGGAATTGGCTCCAGCACCCGGCAGGGTCAATGTGGTCTCCCCGAGCCTGGAGGTT
GCACCAGGTGCAGGACAAGGAGCATCGTCCAGCAGGCCTGACCCTGAGCCCTTAGAGGAA
GGCACGAGGCTGACTCCAGGGCCTGGCCCTCAGTGCCCAGGGCCTCCAGGGCTGCCAGCC
CAGGCCAGGGCAACCCACAGTGGTGGAGAAACACCTCCAAGGATCTCCATCCACATACAA
GAGATGGATACTCCTGGGGAGATGCTGATGACAGGCAGGGGCAGCCTTGGACCCACCCTC
ACCACAGAGGCTCCAGCAGCTGCCCAGCCAGGCAAGCAGGGCCCACCTGGGACCGGGCGC
TGCCTCCAAGCCCCTGGGACTGAGCCCGGAGAACAGACCCCTGAAGGAGCCAGAGAGCTC
TCCCCGCTGCAGGAGAGCAGCAGCCCCGGGGGAGTGAAGGCAGAGGAGGAGCAAAGGGCT
GGGGCCGAGCCTGGCACGAGACCAAGCTTGGCCAGGAGTGACGACAATGACCACGAGGTT
GGGGCCCTGGGCCTGCAGCAGGGCAAAAGCCCAGGGGCGGGAAACCCTGAGCCTGAGCAG
GACTGTGCAGCCAGGGCTCCGGTGAGAGCTGAAGCAGTAAGGAGGATGCCCCCAGGCGCC
GAGGCTGGCAGCGTGGTTCTGGATGACAGTCCGGCCCCACCAGCTCCTTTTGAACACCGG
GTAGTGAGCGTCAAGGAGACCTCCATCTCTGCGGGTTACGAGGTGTGCCAGCACGAAGTC
TTGGGAGGGGGTCGGTTTGGCCAGGTCCACAGGTGCACAGAGAAGTCCACAGGCCTCCCA
CTGGCTGCCAAGATCATCAAAGTGAAGAGCGCCAAGGACCGGGAGGACGTGAAGAACGAG
ATCAACATCATGAACCAGCTCAGCCACGTGAACCTGATCCAGCTCTATGACGCCTTCGAG
AGCAAGCACAGCTGCACCCTTGTCATGGAGTACGTGGACGGGGGTGAGCTCTTCGACCGG
ATCACAGATGAGAAGTACCACCTGACTGAGCTGGATGTGGTCCTGTTCACCAGGCAGATC
TGTGAGGGTGTGCATTACCTGCACCAGCACTACATCCTGCACCTGGACCTCAAGCCGGAG
AACATATTGTGCGTCAATCAGACAGGACATCAAATTAAGATCATTGACTTTGGGCTGGCC
AGAAGGTACAAGCCTCGAGAGAAGCTGAAGGTGAACTTCGGCACTCCTGAGTTCCTGGCC
CCAGAAGTCGTCAATTATGAGTTTGTCTCATTCCCCACAGACATGTGGAGTGTGGGAGTC
ATCACCTACATGCTACTCAGTGGCTTGTCCCCATTTCTAGGGGAAACAGATGCAGAGACC
ATGAATTTCATTGTAAACTGTAGCTGGGATTTTGATGCTGACACCTTTGAAGGGCTCTCG
GAGGAGGCCAAGGACTTTGTTTCCCGGTTGCTGGTCAAAGAGAAGAGCTGCAGAATGAGT
GCCACACAGTGCCTGAAACACGAGTGGCTGAATAATTTGCCTGCCAAAGCTTCAAGATCC
AAAACTCGTCTCAAATCCCAACTACTGCTGCAGAAATACATAGCTCAAAGAAAATGGAAG
AAACATTTCTATGTGGTGACTGCTGCCAACAGGTTAAGGAAATTTCCAACTTCTCCC
Restriction Sites Please inquire |
ACCN NM_182493
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182493.2, NP_872299.2
RefSeq Size 7998 bp
RefSeq ORF 2460 bp
Locus ID 91807
UniProt ID Q32MK0
Cytogenetics 16q11.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
Summary Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 PubMed 18202317).supplied by OMIM, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
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"NM_182493" in other vectors (8)
SKU Description Size Price
RC222246 MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$589.00 $750.00
RC222246L1 Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RC222246L2 Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RC222246L3 Lenti-ORF clone of MYLK3 (Myc-DDK-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RC222246L4 Lenti-ORF clone of MYLK3 (mGFP-tagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$1,050.00
RG222246 MYLK3 (tGFP-tagged) - Human myosin light chain kinase 3 (MYLK3) 10 ug
$789.00 $950.00
SC307402 MYLK3 (untagged)-Human myosin light chain kinase 3 (MYLK3) 10 ug
$802.00
SC323576 MYLK3 (untagged)-Kinase deficient mutant (K520M) of Human myosin light chain kinase 3 (MYLK3) 10 ug
$729.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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