EXD3 (NM_017820) Human Tagged ORF Clone
SKU
RC222174
EXD3 (Myc-DDK-tagged)-Human exonuclease 3'-5' domain containing 3 (EXD3)
-
TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | EXD3 |
Synonyms | mut-7; Nbr |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC222174 representing NM_017820
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCAGGAGATCCCGCTGGTGACCCTGCCGCTGGCGAGCGCCACCGCATGGGCCAGGACCCCCTCC TGCTCCTGCAGGCCCTGCAGACCCTGTGGTCCACGCGGGAGCGGAAGCAGCTCCGGGAGGAAGCCTGGCG GGGGTTTGCTGCCTTGGACGACCCCCTGGCCGGGCTTCTGGACATGCTGGAGAGCTGCCGGGGCCAGCGG GGAGAGGGCCCCTCCCTGGCGGCCTGGATCTCCCACCAGCTGCAGTGCTGGCTACAGGCACAGCCATGCC CGAGCCTGGCCCAGCACAGCCTGAGGCTGAAGCAGCTGCAGGCCCGAGCGGTCAAAGTCCTCACTGAGAG CCCCCCCAGCCTTGCGGCACCACTGGCCAGCATCTTCCAGCTGCAGGATGCAGACAGGAGCTGCCTGCTG GCACACGTCCACCGCCTCCACCACGAGGGCAGGTTCAGAGAAGCAGCCACGCTGGGCACGACGTTGAAGC TGCAGTCGGAGCTTGGCGTTGAAAAGATGAGCATCCCACTGCTCCTCCAGGACAAGGTGGCCCTCGTGGA GCGCTATGTGGCCGGCTTCCCGGACCTCCAGAGGAGGCTGCTGGTCCTCATGGATTCCTGGTGCCAGCCC GGCTTTGACATCAAGGACGTTGCCAGACGGTACCCTGAGGTGACCTCCTTGAGCCTGGAGAAGCTGAGTC CGAAGGCGCTGAGCAGGCAGGTCTTGCGTCTGCAGGAGCGGTACGGCGTAGCCCCAGCCCTGTGTCCCAA CGCGGCCATTCAGCAGCGCCTGGCGGCCCTGCGGCACCTGTGCCACAAGCGGTTTGTGGAGAAGAGCCTG TCACAGGAGAACTGGACCGACCATGTGCAGGGCCTGGTGGGGCAGAGCCCGTGGCTTCAGGAGCAGCTGT CTCAGCTGCTGGTCTCCCACAGTGACCCAGTCACGGCCGCCCAGTGTGCCATGGAACTCTTGCTGCCCGA GGAGCGGCTGCCGGCTGCGGTGGCTGTGGAACTCCGCCGGTTCAGGCTCCAGGGGAGGGCGACTGAGGCT GACTCGAGGCTGGAGGTGAAGGACATGAAGGACCGTTACTACCAGCTGCCCATCCCCAGGGAGAACGTCC ACCTCCTGGCCTCGTGGGAAGACCTGACCAGACACGAGGGTGCACTCCTGCAGTGCCACCAAGTGGTTGG TGTAGACGTGGAGTGGACACCTGTGTTTGTTGCTGGGGGCCGGCCTCGGCCGTCACTCCTGCAGGTGGCC GTGGAGGGCCACGTGTTCCTTCTGGACGTCCTGGCACTCTCGCAGCCACCAACAGGGCAGGGAGCCCAGG CCTTTTCCCGGCTGGTGGCCCAGCTCCTCTCGGACCCCTCTATCACCAAGCTGGGCTACGGGATGGTGGG GGACCTGCAAAAACTGGGCACGTCCTGCCCCGCCCTGGCCCATGTGGAGAAGCAGATTCTGGGCGGCATG GACCTGCTGCTGGTGCACAGACAGATGCGGGTGGCGAGCGTGCCAGCCCCAGCCGTGGACAGGGCCAGGG AGCTGAGGGGCCTGAGCCTCCTGGTGCAGCAGGTGCTGGGCACAGCCCTGGACAAGACGCAGCAGCTGTC CAACTGGGACCGGAGGCCGCTCTACGAGGAGCAGGTCATCTACGCAGCTGCCGACGCCTACTGCCTGCTG GAGGTGCACCAAGCCCTGTGCAGAGAGCCCGCCCGCTTCCACCTGTCGGAGGACCTGGCTGGGAGCCGGA GGCCCAGGCACAGAGAGAGACCAGGGGCACGGAAGCCACCCGGCCTGCAGAAAGCGTCAGCACCGGCCGC ACCCAGGCAGGTCCCTGTGGCTGTGGCTGTGTCTGAGGGCGCTGCCCCTCAGATTCCGGCCAGGGCCTTC CGTGTGGTGTGTGACAACATGCTGCAGGGGCTGGCACGGAGCCTCCGCTGTCTCGGTGTGGATGCACGCA TGCTGGGCAATGGTGAAGACCACCGCAGGGCGGCCGAGGTTGCCAGGCAGGAGGGGAGGATCATTCTGAC GTCGGGGCAGCCATTCCACAAGCTCCGGGCCCAGGTCGGGGCTGGGCGCTGCCTCTCGGTCGACTGCTCC CTGAAGGCCCAGCAGCAGGCCAAGGCTGTGCTCAAGCATTTCAACGTGCGTGTCACCCACGCAGACATCT TCAGCCGCTGCCAGGCCTGTAACTGTGACCAGTACCTAAAGGTCTCCAGGGACATGATGAAGCAGCTCAT GTGGCTCAGCAGTCACCAGGAGGGGCCCAGGAGCTCAGGTGACGAGGCCACCCAGAGCCAGGCGGTGCAG GAGCCAGGCCCAGCCCCAGACGCAGCCCCTGAGGGCTGCACCTATGACCGCCCCTGCCGCTGGCTGCAGA TGGCTGACCTGCGGGCGGAGACACCGGACATGCTGGCCGACGGCACCCGGCTGCAGCTGGCAGGGGTCCC GGTGGGTGTGCTGAGGACACCTGGGCTGCGGTGCTTCTACTGCTGCACGGGCTGTGGAAAGGTCTTCTGG GACGGCTCCCACCTGGGTCGTGTTGCCACCCACTTCCGAGACATGCTGGAGAGCGCCCCCAGCCCCTGCG AGCCGAGCCCAGCCCCCAGCCCGGCCAGCAGTCCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222174 representing NM_017820
Red=Cloning site Green=Tags(s) MDPGDPAGDPAAGERHRMGQDPLLLLQALQTLWSTRERKQLREEAWRGFAALDDPLAGLLDMLESCRGQR GEGPSLAAWISHQLQCWLQAQPCPSLAQHSLRLKQLQARAVKVLTESPPSLAAPLASIFQLQDADRSCLL AHVHRLHHEGRFREAATLGTTLKLQSELGVEKMSIPLLLQDKVALVERYVAGFPDLQRRLLVLMDSWCQP GFDIKDVARRYPEVTSLSLEKLSPKALSRQVLRLQERYGVAPALCPNAAIQQRLAALRHLCHKRFVEKSL SQENWTDHVQGLVGQSPWLQEQLSQLLVSHSDPVTAAQCAMELLLPEERLPAAVAVELRRFRLQGRATEA DSRLEVKDMKDRYYQLPIPRENVHLLASWEDLTRHEGALLQCHQVVGVDVEWTPVFVAGGRPRPSLLQVA VEGHVFLLDVLALSQPPTGQGAQAFSRLVAQLLSDPSITKLGYGMVGDLQKLGTSCPALAHVEKQILGGM DLLLVHRQMRVASVPAPAVDRARELRGLSLLVQQVLGTALDKTQQLSNWDRRPLYEEQVIYAAADAYCLL EVHQALCREPARFHLSEDLAGSRRPRHRERPGARKPPGLQKASAPAAPRQVPVAVAVSEGAAPQIPARAF RVVCDNMLQGLARSLRCLGVDARMLGNGEDHRRAAEVARQEGRIILTSGQPFHKLRAQVGAGRCLSVDCS LKAQQQAKAVLKHFNVRVTHADIFSRCQACNCDQYLKVSRDMMKQLMWLSSHQEGPRSSGDEATQSQAVQ EPGPAPDAAPEGCTYDRPCRWLQMADLRAETPDMLADGTRLQLAGVPVGVLRTPGLRCFYCCTGCGKVFW DGSHLGRVATHFRDMLESAPSPCEPSPAPSPASSPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017820 |
ORF Size | 2628 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_017820.5 |
RefSeq Size | 2887 bp |
RefSeq ORF | 2631 bp |
Locus ID | 54932 |
UniProt ID | Q8N9H8 |
Cytogenetics | 9q34.3 |
MW | 96.4 kDa |
Summary | Possesses 3'-5' exoribonuclease activity. Required for 3'-end trimming of AGO1-bound miRNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC222174L1 | Lenti-ORF clone of EXD3 (Myc-DDK-tagged)-Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$1,102.00
|
|
RC222174L2 | Lenti-ORF clone of EXD3 (mGFP-tagged)-Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$1,102.00
|
|
RC222174L3 | Lenti-ORF clone of EXD3 (Myc-DDK-tagged)-Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$1,102.00
|
|
RC222174L4 | Lenti-ORF clone of EXD3 (mGFP-tagged)-Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$1,102.00
|
|
RG222174 | EXD3 (tGFP-tagged) - Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$1,002.00
|
|
SC318352 | EXD3 (untagged)-Human exonuclease 3'-5' domain containing 3 (EXD3) | 10 ug |
$883.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.