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SP3 (NM_003111) Human Tagged ORF Clone

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SKU
RC222027
SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $715.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SP3 or NM_003111"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SP3
Synonyms SPR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222027 representing NM_003111
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGCTCCCGAAAAGCCCGTGAAACAAGAGGAAATGGCTGCCTTGGACGTGGATAGCGGCGGCGGCG
GTGGCGGCGGCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCACGGAAACGGTGCGGTGGCGGC
GGCAGCGGCGGCCCAGGACACTCAGCCGTCACCGCTCGCTCTGCTGGCCGCTACCTGCAGCAAGATAGGG
CCGCCATCGCCGGGCGACGACGAGGAGGAGGCGGCCGCCGCAGCCGGGGCCCCCGCCGCCGCCGGAGCGA
CAGGTGATTTGGCTTCTGCACAGTTAGGAGGAGCACCAAACCGATGGGAGGTTTTGTCAGCCACACCTAC
AACTATAAAAGATGAAGCTGGTAATCTAGTCCAGATTCCAAGTGCTGCTACTTCAAGTGGGCAGTATGTT
CTTCCCCTTCAGAATTTGCAGAATCAACAAATATTTTCCGTTGCACCAGGATCAGATTCATCAAATGGTA
CAGTGTCCAGTGTTCAATATCAAGTGATACCACAGATCCAGTCAGCAGATGGTCAGCAGGTTCAAATTGG
TTTCACAGGCTCTTCAGATAATGGGGGTATAAATCAAGAAAGCAGTCAAATTCAGATCATTCCTGGCTCT
AATCAAACCTTACTTGCCTCTGGAACACCTTCTGCTAACATCCAGAATCTCATACCACAGACTGGTCAAG
TCCAGGTTCAGGGAGTTGCAATTGGTGGTTCATCTTTTCCTGGTCAAACCCAAGTAGTTGCTAATGTGCC
TCTTGGTCTGCCAGGAAATATTACGTTTGTACCAATCAATAGTGTCGATCTAGATTCTTTGGGACTCTCG
GGCAGTTCTCAGACAATGACTGCAGGCATTAATGCCGACGGACATTTGATAAACACAGGACAAGCTATGG
ATAGTTCAGACAATTCAGAAAGGACTGGTGAGCGGGTTTCTCCTGATATTAATGAAACTAATACTGATAC
AGATTTATTTGTGCCAACATCCTCTTCATCACAGTTGCCTGTTACGATAGATAGTACAGGTATATTACAA
CAAAACACAAATAGCTTGACTACATCTAGTGGGCAGGTTCATTCTTCAGATCTTCAGGGAAATTATATCC
AGTCGCCTGTTTCTGAAGAGACACAGGCACAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAGCA
TCTACAACTTCAAGAGTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACA
ATCCATGGTGTGCAAGCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAATCTTCAGTTGCAGCTGA
ATCCTGGAACCTTTTTAATTCAGGCACAGACAGTGACCCCTTCTGGACAGGTAACTTGGCAAACGTTTCA
AGTACAAGGGGTCCAGAACTTGCAGAATTTGCAAATACAGAATACTGCTGCCCAACAAATAACTTTGACG
CCTGTTCAAACCCTCACACTTGGTCAAGTTGCGGCAGGTGGAGCCTTCACTTCAACTCCAGTTAGTCTAA
GCACTGGTCAGTTGCCAAATCTACAAACAGTTACAGTGAACTCTATAGATTCTGCTGGTATACAGCTACA
TCCAGGAGAGAATGCTGACAGTCCTGCAGATATTAGGATCAAGGAAGAAGAACCTGATCCTGAAGAGTGG
CAGCTCAGTGGTGATTCTACCTTGAATACCAATGACCTAACACACTTAAGAGTACAGGTGGTAGATGAAG
AAGGGGACCAACAACATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGCACCTGTCCCAACTGTAAAGA
AGGTGGTGGAAGAGGTACCAATCTTGGGAAAAAGAAGCAACACATTTGTCATATACCAGGATGTGGTAAA
GTCTATGGGAAGACCTCACATCTGAGAGCTCATCTGCGTTGGCATTCTGGAGAACGCCCTTTTGTTTGTA
ACTGGATGTACTGTGGTAAAAGATTTACTCGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGG
TGAGAAGAAATTTGTTTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATT
AAAACACACCAGAATAAAAAAGGTATTCACTCTAGCAGTACAGTGCTGGCATCTGTGGAAGCTGCGCGAG
ATGATACTTTGATTACTGCAGGAGGAACAACGCTTATCCTTGCAAATATTCAACAAGGTTCTGTTTCAGG
GATAGGAACTGTTAATACTTCCGCCACCAGCAATCAAGATATCCTTACCAACACTGAAATACCTTTACAG
CTTGTCACAGTTTCTGGAAATGAGACAATGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC222027 protein sequence
Red=Cloning site Green=Tags(s)

MTAPEKPVKQEEMAALDVDSGGGGGGGGGHGEYLQQQQQHGNGAVAAAAAAQDTQPSPLALLAATCSKIG
PPSPGDDEEEAAAAAGAPAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPSAATSSGQYV
LPLQNLQNQQIFSVAPGSDSSNGTVSSVQYQVIPQIQSADGQQVQIGFTGSSDNGGINQESSQIQIIPGS
NQTLLASGTPSANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLS
GSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQ
QNTNSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQT
IHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQVTWQTFQVQGVQNLQNLQIQNTAAQQITLT
PVQTLTLGQVAAGGAFTSTPVSLSTGQLPNLQTVTVNSIDSAGIQLHPGENADSPADIRIKEEEPDPEEW
QLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGK
VYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHI
KTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQ
LVTVSGNETME

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_003111
ORF Size 2343 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003111.5
RefSeq Size 3920 bp
RefSeq ORF 2346 bp
Locus ID 6670
UniProt ID Q02447, Q86TP0
Cytogenetics 2q31.1
Protein Families Druggable Genome, Transcription Factors
MW 81.7 kDa
Summary This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. provided by RefSeq, Feb 2010
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"NM_003111" in other vectors (6)
SKU Description Size Price
RC222027L1 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged 10 ug
$1,015.00
RC222027L2 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged 10 ug
$1,015.00
RC222027L3 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged 10 ug
$1,015.00
RC222027L4 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged 10 ug
$1,015.00
RG222027 SP3 (tGFP-tagged) - Human Sp3 transcription factor (SP3), transcript variant 1 10 ug
$789.00 $915.00
SC121408 SP3 (untagged)-Human Sp3 transcription factor (SP3), transcript variant 1 10 ug
$716.00

file-alt Citations

A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
Hiramoto, Udagawa, Ishibashi et al
Int J Mol Med (2018) 41 (2), 717-728 DOI: 10.3892/ijmm.2017.3273
Cited by 17
AI Summary: In this study, OriGene products—specifically the human SP3 shRNA plasmid (TL313206) and human KDM1A (LSD1) shRNA plasmid (TL313865)—were used to knock down SP3 and KDM1A gene expression, enabling the authors to investigate how these proteins interact with the KCNQ1 SNP rs163184 locus and affect CDKN1C gene expression.
FigureFigureFigure
Differential effects of Sp cellular transcription factors on viral promoter activation by varicella-zoster virus (VZV) IE62 protein
Khalil, Ruyechan, Hay et al
Virology (2015) 485, 47-57 DOI: 10.1016/j.virol.2015.06.031
Cited by 7
AI Summary: OriGene products contributed to this study by providing the pCMV-Sp1, pCMV-Sp2, pCMV-Sp3, and pCMV-Sp4 expression plasmids, which were used to analyze the effects of Sp family transcription factors on VZV IE62-mediated viral promoter activation.
Snippet: The pCMV62-d20 was derived from pCMV62 by site-directed mutagenesis using the QuikChange site-directed mutagenesis kit (Stratagene, Cedar Creek, TX) to delete 20 amino acids (238–258) in the ORF62 gene. The pCMV-Sp1, pCMV-Sp2, pCMV-Sp3 and pCMV-Sp4 expressing plasmids were purchased from Origene Technologies (Rockville, MD)
Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation
Kasaai, Gaumond, Moffatt
J. Biol. Chem. (2013) 288 (19), 13278-94 DOI: 10.1074/jbc.M113.457010
Cited by 2
AI Summary: OriGene products contributed to this study by providing plasmids for human Sp3 variant 1 (Sp3-L1, rc222027) and human Sp3 variant 2 (Sp3-L2, rc220658), which were used to investigate the role of Sp3 isoforms in regulating BRIL gene transcription.
Snippet: The following plasmids were purchased from Origene: human Sp3 variant 1 (Sp3-L1) (rc222027), human Sp3 variant 2 (Sp3-L2) (rc220658), human ... with T4 polynucleotide kinase (New England Biolabs) and 50 μCi of [γ- 32 P]ATP (3000 Ci/mmol) (PerkinElmer Life Sciences) at
Showing 1-3 of 3 papers.
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