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SP3 (NM_003111) Human Untagged Clone

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SKU
SC121408
SP3 (untagged)-Human Sp3 transcription factor (SP3), transcript variant 1
  $716.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SP3
Synonyms SPR2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC121408 sequence for NM_003111 edited (data generated by NextGen Sequencing)
ATGACCGCTCCCGAAAAGCCCGTGAAACAAGAGGAAATGGCTGCCTTGGACGTGGATAGC
GGCGGCGGCGGTGGCGGCGGCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCAC
GGAAACGGTGCGGTGGCGGCGGCAGCGGCGGCCCAGGACACTCAGCCGTCACCGCTCGCT
CTGCTGGCCGCTACCTGCAGCAAGATAGGGCCGCCATCGCCGGGCGACGACGAGGAGGAG
GCGGCCGCCGCAGCCGGGGCCCCCGCCGCCGCCGGAGCGACAGGTGATTTGGCTTCTGCA
CAGTTAGGAGGAGCACCAAACCGATGGGAGGTTTTGTCAGCCACACCTACAACTATAAAA
GATGAAGCTGGTAATCTAGTCCAGATTCCAAGTGCTGCTACTTCAAGTGGGCAGTATGTT
CTTCCCCTTCAGAATTTGCAGAATCAACAAATATTTTCCGTTGCACCAGGATCAGATTCA
TCAAATGGTGCAGTGTCCAGTGTTCAATATCAAGTGATACCACAGATCCAGTCAGCAGAT
GGTCAGCAGGTTCAAATTGGTTTCACAGGCTCTTCAGATAATGGGGGTATAAATCAAGAA
AGCAGTCAAATTCAGATCATTCCTGGCTCTAATCAAACCTTACTTGCCTCTGGAACACCT
TCTGCTAACATCCAGAATCTCATACCACAGACTGGTCAAGTCCAGGTTCAGGGAGTTGCA
ATTGGTGGTTCATCTTTTCCTGGTCAAACCCAAGTAGTTGCTAATGTGCCTCTTGGTCTG
CCAGGAAATATTACGTTTGTACCAATCAATAGTGTCGATCTAGATTCTTTGGGACTCTCG
GGCAGTTCTCAGACAATGACTGCAGGCATTAATGCCGACGGACATTTGATAAACACAGGA
CAAGCTATGGATAGTTCAGACAATTCAGAAAGGACTGGTGAGCGGGTTTCTCCTGATATT
AATGAAACTAATACTGATACAGATTTATTTGTGCCAACATCCTCTTCATCACAGTTGCCT
GTTACGATAGATAGTACAGGTATATTACAACAAAACACAAATAGCTTGACTACATCTAGT
GGGCAGGTTCATTCTTCAGATCTTCAGGGAAATTATATCCAGTCGCCTGTTTCTGAAGAG
ACACAGGCACAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAGCATCTACAACTT
CAAGAGTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACA
ATCCATGGTGTGCAAGCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAATCTTCAG
TTGCAGCTGAATCCTGGAACCTTTTTAATTCAGGCACAGACAGTGACCCCTTCTGGACAG
GTAACTTGGCAAACGTTTCAAGTACAAGGGGTCCAGAACTTGCAGAATTTGCAAATACAG
AATACTGCTGCCCAACAAATAACTTTGACGCCTGTTCAAACCCTCACACTTGGTCAAGTT
GCGGCAGGTGGAGCCTTCACTTCAACTCCAGTTAGTCTAAGCACTGGTCAGTTGCCAAAT
CTACAAACAGTTACAGTGAACTCTATAGATTCTGCTGGTATACAGCTACATCCAGGAGAG
AATGCTGACAGTCCTGCAGATATTAGGATCAAGGAAGAAGAACCTGATCCTGAAGAGTGG
CAGCTCAGTGGTGATTCTACCTTGAATACCAATGACCTAACACACTTAAGAGTACAGGTG
GTAGATGAAGAAGGGGACCAACAACATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGC
ACCTGTCCCAACTGTAAAGAAGGTGGTGGAAGAGGTACCAATCTTGGGAAAAAGAAGCAA
CACATTTGTCATATACCAGGATGTGGTAAAGTCTATGGGAAGACCTCACATCTGAGAGCT
CATCTGCGTTGGCATTCTGGAGAACGCCCTTTTGTTTGTAACTGGATGTACTGTGGTAAA
AGATTTACTCGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGGTGAGAAGAAA
TTTGTTTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATT
AAAACACACCAGAATAAAAAAGGTATTCACTCTAGCAGTACAGTGCTGGCATCTGTGGAA
GCTGCGCGAGATGATACTTTGATTACTGCAGGAGGAACAACGCTTATCCTTGCAAATATT
CAACAAGGTTCTGTTTCAGGGATAGGAACTGTTAATACTTCCGCCACCAGCAATCAAGAT
ATCCTTACCAACACTGAAATACCTTTACAGCTTGTCACAGTTTCTGGAAATGAGACAATG
GAGTAA

Clone variation with respect to NM_003111.4
490 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003111 unedited
GGGGGTCACATAGNAAACCCTTCCTATAGGCGGCCGCGNAATTCGCACGAGCCGGCCGTG
TCCGCCGCCGCTTCCCGCATCCTCTCCCGCCGCCGCCGCCTTCGCTCCTCACCATGTGTA
AGGCGGCGGGGAGCCCCGCCTGAGTGCCCTAAACACACTATGACCGCTCCCGAAAAGCCC
GTNGAAACAAGAGGAAATGGCTGCCTTGGTTCTTTTATAGCGGCGGCGGCGGTGGCGGCG
GCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCACGGAAACGGTGCGGTGGCGG
CGGCAGCGGCGGCCCAGGACACTCAGCCGTCACCGCTCGCTCTGCTGGCCGCTACCTGCA
GCAAGATAGGGCCGCCATCGCCGGGCGACGACGAGGAGGAGGCGGCCGCCGCAGCCGGGG
CCCCCGCCGCCGCCGGAGCGACAGGTGATTTGGCTTCTGCACAGTTTGGAGGAGCACCAA
ACCGATGGGAGGTTTTGTCAGCCACACCTACAACTTNAAAGATGAAGCTGGTAATCTAGT
CCAGATTCCAAGTGCTGCTTCTTCCAGTGGGCAGTATGTTCTTCCCCTTCCTAATTTGCG
GAATCACATATATTTTCCGTTGCCCCGGGTTCAGATTCCTCAAATGGTGCAGTGTCCCAT
GTTTCATTTCATGTGGTCCCCCCGATCCCGTCCGCGCTGGGTCCCCAGGTTCAAATTTGG
TTTCACAGGCTTTTCCGATAATGGGGGGGTTTAATCCAGGAAAGCGTTCCATTTCGGTTC
TTCCTGGCTCTTTATTAACCCTTATTTGGCCTTTGGAACCCCTTTCGGTTTCTCTCCCCA
ATCCCTTCCCCCCACCTGGTTCACTCCCACGTTCAGGGGCTGCCAACTTGGGAGTAAAAA
AATA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003111 unedited
TTAGAATACACCCTAGACGATGACCATAAATGAGACACCATCTAGATTTAGAGGATGCGC
TCTCTAAAATATAGCAAATGAACAGCAATTCCAAATTTTCTGAAATATGTGAACTGCTGC
AATTACAAGTATACATTTAGGAAAACTGTCTAGCTCTTACAAGACCAGCAATGTAACTTT
ATTTTGTACATTTTTGAATTGAAAATATAAACAATAATTAAAAAATAAAAAGAAAATACA
GCATAATAAAAAACATACGCTTCTCAATTAAATGTACTGCATACATATAAATTTTAAGGG
AAGAAGCACAAAAGGAAAATGATTGATATTTAAGTGCAGACTGACTACCTACACCAAAAA
TAAAAAGACTTAAAAAAATATCCTAAAACCTCTAGTTCTTCTATGACTAATATCCATATG
GTTGGAGTATCGTCACTATGGAACTGATTTTGTTAAGTTTGCCTATGTTACAGTTTACTG
TTAATTTTCATGATGGCTGTTAAAGCCCTGAGAGACAAGGTTTTAGGTATAAAGATGGAT
ACAAATAACAGTTAAAACGAAGGACAGATTACTTATCCGGTCCCCCAGGAGTCAAGAAAT
GGGACCATTGACGATAAGATTGCGCCTAACACCGAAGGGCCCTCACTTAATCCAAACTGA
ACCCTCTATAAAAGCTAGGTCCTTCGGCCATCCCGCGTGTTCACCAAAGCTCTATCCACC
CCCTCATTTTCCGGGAGTGCATCCTTATACCAGGTCATTGTCAAACACCATCCCACGGTA
GACCTATCCAACCGGGTTGCTGTCAACGCAAAGACAGTCCAATAAGTTCGACCACTAGAA
TCATCTATAGCATTTGTAATTTATCATAGATCCAGTGCTCACCAATAGCCTATAAACAGA
TCTTATATCTT
Restriction Sites NotI-NotI |
ACCN NM_003111
Insert Size 4400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003111.3, NP_003102.1
RefSeq Size 3920 bp
RefSeq ORF 2346 bp
Locus ID 6670
UniProt ID Q02447
Cytogenetics 2q31.1
Protein Families Druggable Genome, Transcription Factors
Summary This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. provided by RefSeq, Feb 2010
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Alternative translation initiation from downstream, in-frame start codons produces shorter isoforms. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_003111" in other vectors (6)
SKU Description Size Price
RC222027 SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 1 10 ug
$715.00
RC222027L1 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged 10 ug
$1,015.00
RC222027L2 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged 10 ug
$1,015.00
RC222027L3 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged 10 ug
$1,015.00
RC222027L4 Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged 10 ug
$1,015.00
RG222027 SP3 (tGFP-tagged) - Human Sp3 transcription factor (SP3), transcript variant 1 10 ug
$789.00 $915.00

file-alt Citations

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