SMARCD3 (NM_001003801) Human Tagged ORF Clone

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SKU
RC222004
SMARCD3 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (SMARCD3), transcript variant 3
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SMARCD3
Synonyms BAF60C; CRACD3; Rsc6p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC222004 representing NM_001003801
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGGACGAAGTTGCCGGAGGGGCGCGCAAAGCCACGAAAAGCAAACTTTTTGAGTTTCTGGTCC
ATGGGGTGCGCCCCGGGATGCCGTCTGGAGCCCGGATGCCCCACCAGGGGGCGCCCATGGGCCCCCCGGG
CTCCCCGTACATGGGCAGCCCCGCCGTGCGACCCGGCCTGGCCCCCGCGGGCATGGAGCCCGCCCGCAAG
CGAGCAGCGCCCCCGCCCGGGCAGAGCCAGGCACAGAGCCAGGGCCAGCCGGTGCCCACCGCCCCCGCGC
GGAGCCGCAGTGCCAAGAGGAGGAAGATGGCTGACAAAATCCTCCCTCAAAGGATTCGGGAGCTGGTCCC
CGAGTCCCAGGCTTACATGGACCTCTTGGCATTTGAGAGGAAACTGGATCAAACCATCATGCGGAAGCGG
GTGGACATCCAGGAGGCTCTGAAGAGGCCCATGAAGCAAAAGCGGAAGCTGCGACTCTATATCTCCAACA
CTTTTAACCCTGCGAAGCCTGATGCTGAGGATTCCGACGGCAGCATTGCCTCCTGGGAGCTACGGGTGGA
GGGGAAGCTCCTGGATGATCCCAGCAAACAGAAGCGGAAGTTCTCTTCTTTCTTCAAGAGTTTGGTCATC
GAGCTGGACAAAGATCTTTATGGCCCTGACAACCACCTCGTTGAGTGGCATCGGACACCCACGACCCAGG
AGACGGACGGCTTCCAGGTGAAACGGCCTGGGGACCTGAGTGTGCGCTGCACGCTGCTCCTCATGCTGGA
CTACCAGCCTCCCCAGTTCAAACTGGATCCCCGCCTAGCCCGGCTGCTGGGGCTGCACACACAGAGCCGC
TCAGCCATTGTCCAGGCCCTGTGGCAGTATGTGAAGACCAACAGGCTGCAGGACTCCCATGACAAGGAAT
ACATCAATGGGGACAAGTATTTCCAGCAGATTTTTGATTGTCCCCGGCTGAAGTTTTCTGAGATTCCCCA
GCGCCTCACAGCCCTGCTATTGCCCCCTGACCCAATTGTCATCAACCATGTCATCAGCGTGGACCCTTCA
GACCAGAAGAAGACGGCGTGCTATGACATTGACGTGGAGGTGGAGGAGCCATTAAAGGGGCAGATGAGCA
GCTTCCTCCTATCCACGGCCAACCAGCAGGAGATCAGTGCTCTGGACAGTAAGATCCATGAGACGATTGA
GTCCATAAACCAGCTCAAGATCCAGAGGGACTTCATGCTAAGCTTCTCCAGAGACCCCAAAGGCTATGTC
CAAGACCTGCTCCGCTCCCAGAGCCGGGACCTCAAGGTGATGACAGATGTAGCCGGCAACCCTGAAGAGG
AGCGCCGGGCTGAGTTCTACCACCAGCCCTGGTCCCAGGAGGCCGTCAGTCGCTACTTCTACTGCAAGAT
CCAGCAGCGCAGGCAGGAGCTGGAGCAGTCGCTGGTTGTGCGCAACACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC222004 representing NM_001003801
Red=Cloning site Green=Tags(s)

MAADEVAGGARKATKSKLFEFLVHGVRPGMPSGARMPHQGAPMGPPGSPYMGSPAVRPGLAPAGMEPARK
RAAPPPGQSQAQSQGQPVPTAPARSRSAKRRKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIMRKR
VDIQEALKRPMKQKRKLRLYISNTFNPAKPDAEDSDGSIASWELRVEGKLLDDPSKQKRKFSSFFKSLVI
ELDKDLYGPDNHLVEWHRTPTTQETDGFQVKRPGDLSVRCTLLLMLDYQPPQFKLDPRLARLLGLHTQSR
SAIVQALWQYVKTNRLQDSHDKEYINGDKYFQQIFDCPRLKFSEIPQRLTALLLPPDPIVINHVISVDPS
DQKKTACYDIDVEVEEPLKGQMSSFLLSTANQQEISALDSKIHETIESINQLKIQRDFMLSFSRDPKGYV
QDLLRSQSRDLKVMTDVAGNPEEERRAEFYHQPWSQEAVSRYFYCKIQQRRQELEQSLVVRNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001003801
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001003801.2
RefSeq Size 1700 bp
RefSeq ORF 1452 bp
Locus ID 6604
UniProt ID Q6STE5
Cytogenetics 7q36.1
Protein Families Druggable Genome, Transcription Factors
MW 54.8 kDa
Summary The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001003801" in other vectors (4)
SKU Description Size Price
RC222004L3 Lenti-ORF clone of SMARCD3 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (SMARCD3), transcript variant 3 10 ug
$757.00
RC222004L4 Lenti-ORF clone of SMARCD3 (mGFP-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (SMARCD3), transcript variant 3 10 ug
$757.00
RG222004 SMARCD3 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (SMARCD3), transcript variant 3 10 ug
$657.00
SC300553 SMARCD3 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (SMARCD3), transcript variant 3 10 ug
$457.00

Plasmid Map for RC222004

Download sequence for

Cloning scheme for RC222004

Circular map for RC222004

file-alt Citations

SMARCD3 Promotes Epithelial–Mesenchymal Transition in Gastric Cancer by Integrating PI3K-AKT and WNT/β-Catenin Pathways
Park, Park, Jung et al
Cancers (2025) 17 (21), 3526-3526 DOI: 10.3390/cancers17213526
Cited by 1
AI Summary: OriGene products contributed to this study by providing the pCMV6-entry empty vector (cat #: PS100001) and the pCMV6-SMARCD3-FLAG-DDK overexpression plasmid (cat #: RC222004), which were used to manipulate SMARCD3 expression in gastric cancer cell lines to investigate its role in EMT and signaling pathways.
Snippet: For plasmid overexpression, cells were transfected with either a pCMV6-entry empty vector (cat #: PS100001;OriGene Technologies, Rockville, MD, USA) or a pCMV6-SMARCD3 construct tagged with FLAG-DDK (cat #: RC222004;OriGene Technologies, Rockville, MD, USA) using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocol
Showing 1-1 of 1 papers.
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