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MAPKBP1 (NM_014994) Human Tagged ORF Clone

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SKU
RC221849
MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,290.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "MAPKBP1 or NM_014994"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAPKBP1
Synonyms JNKBP-1; JNKBP1; NPHP20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221849 representing NM_014994
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTGGAAGGGTCAACCATTACCAGCCGGATCAAGAATCTGTTGAGATCTCCATCCATCAAACTGC
GCAGGAGTAAGGCAGGAAACCGACGAGAGGACCTCAGCTCCAAGGTGACCTTGGAGAAGGTGCTGGGAAT
TACAGTGTCTGGAGGCAGAGGACTTGCCTGTGACCCCCGATCAGGTTTAGTTGCTTACCCAGCAGGGTGT
GTGGTTGTGTTGTTCAATCCCCGGAAACACAAACAGCACCACATCCTCAACAGTTCCAGGAAAACCATCA
CTGCCCTTGCCTTCTCCCCTGATGGCAAGTACTTGGTCACTGGAGAGAGTGGGCACATGCCTGCCGTGCG
GGTTTGGGACGTGGCAGAGCACAGCCAGGTGGCCGAGCTGCAGGAGCACAAGTATGGTGTGGCTTGTGTG
GCCTTCTCTCCTAGCGCCAAGTACATTGTCTCTGTGGGCTACCAGCATGACATGATCGTCAACGTGTGGG
CCTGGAAGAAAAACATTGTGGTGGCCTCCAACAAGGTGTCCAGTCGGGTGACAGCAGTGTCCTTCTCTGA
GGATTGCAGCTACTTTGTCACTGCAGGCAACCGACACATCAAATTCTGGTATCTCGATGACAGCAAGACC
TCAAAGGTGAATGCCACTGTGCCCTTGCTGGGCCGCTCAGGGCTGCTGGGAGAGCTACGGAACAACCTAT
TCACTGATGTGGCCTGTGGCAGAGGAAAAAAGGCGGACAGTACCTTCTGCATCACGTCCTCAGGGCTGCT
GTGCGAGTTCAGTGATCGAAGGCTTTTGGACAAGTGGGTGGAGCTGAGGACCACAGTGGCCCACTGCATC
TCTGTGAGCCAAGACTACATCTTCTGTGGCTGTGCTGATGGCACCGTGCGCCTTTTCAACCCCTCTAACC
TGCACTTCCTTAGCACCTTGCCCCGACCCCATGCTCTGGGGACAGACATTGCTAGCGTCACCGAGGCCAG
TCGCCTCTTCTCTGGAGTGGCGAATGCCAGGTATCCAGACACCATTGCCTTGACCTTTGATCCTACTAAT
CAGTGGCTGTCTTGTGTGTACAACGATCATAGCATTTATGTTTGGGATGTGAGGGACCCCAAGAAAGTGG
GCAAGGTGTACTCGGCTCTGTATCATTCTTCCTGCGTCTGGAGTGTGGAGGTCTACCCCGAGGTGAAGGA
TAGTAACCAGGCCTGCCTGCCCCCCAGTTCCTTTATTACCTGCTCCTCAGACAACACCATCCGCCTGTGG
AACACAGAGAGCTCCGGGGTGCATGGCTCCACCCTCCACCGAAACATCCTCAGCAGTGACCTCATTAAAA
TCATCTATGTGGATGGGAACACCCAGGCCCTGCTGGACACAGAGCTGCCTGGAGGAGACAAAGCTGATGC
ATCCCTGTTGGATCCCCGCGTGGGCATCCGCTCGGTGTGTGTCAGCCCCAATGGACAGCATCTAGCATCA
GGGGACCGTATGGGCACACTTAGGGTGCACGAACTTCAGTCCCTGAGTGAGATGCTGAAGGTGGAGGCCC
ATGACTCTGAGATTCTGTGCCTGGAGTATTCTAAGCCAGACACAGGTCTGAAACTGCTAGCATCGGCGAG
CCGGGACCGGCTGATCCATGTGCTGGATGCCGGGCGGGAGTACAGCCTACAGCAGACGCTGGACGAACAC
TCATCCTCCATCACTGCTGTTAAGTTTGCAGCCAGTGATGGGCAAGTCCGCATGATCAGCTGTGGAGCAG
ACAAGAGCATCTACTTCCGCACTGCGCAGAAGTCTGGAGATGGAGTGCAGTTCACACGGACACACCACGT
GGTGCGGAAGACGACCCTCTATGACATGGATGTGGAGCCCAGCTGGAAGTACACGGCTATCGGCTGCCAG
GACCGAAATATTCGGATATTTAACATCAGCAGTGGAAAGCAGAAGAAGCTGTTTAAAGGGTCACAGGGTG
AGGACGGCACACTCATTAAGGTGCAGACAGACCCCTCAGGGATCTACATTGCCACCAGCTGTTCTGACAA
GAATCTCTCCATTTTTGACTTCTCCTCAGGCGAGTGCGTGGCCACCATGTTTGGCCACTCAGAGATTGTC
ACTGGCATGAAATTTAGTAATGATTGTAAACATCTCATCTCTGTGTCTGGGGACAGCTGCATATTTGTGT
GGCGCCTGAGCTCTGAGATGACCATCAGCATGAGGCAGCGTCTGGCCGAGTTGCGCCAGCGTCAGCGGGG
CGGCAAGCAGCAAGGACCATCCTCTCCCCAAAGGGCTTCTGGACCCAACCGGCACCAGGCCCCATCAATG
CTGTCTCCTGGACCGGCTCTCTCATCAGACAGTGACAAGGAGGGAGAAGATGAGGGGACTGAAGAAGAAC
TTCCAGCACTGCCCGTCCTTGCCAAGAGTACCAAGAAGGCACTGGCCTCGGTCCCCAGCCCAGCTTTGCC
CCGAAGCCTGTCCCACTGGGAGATGAGTCGGGCACAGGAGTCCGTGGGGTTCCTGGACCCAGCTCCTGCA
GCCAACCCAGGACCCAGAAGAAGAGGGCGCTGGGTTCAGCCAGGTGTGGAACTGAGCGTTAGATCCATGC
TGGATCTGCGGCAGCTGGAAACACTGGCCCCAAGCCTGCAGGACCCTAGCCAGGACTCGCTGGCCATCAT
CCCATCTGGTCCCAGGAAGCATGGGCAGGAGGCCCTTGAGACTTCACTCACTAGCCAGAATGAAAAGCCC
CCTCGGCCTCAGGCTTCCCAACCTTGTTCCTATCCCCATATTATCCGATTATTGTCACAAGAGGAAGGGG
TCTTTGCCCAAGATCTGGAACCTGCACCCATTGAAGATGGTATTGTCTACCCGGAGCCGAGTGACAACCC
CACCATGGATACCAGTGAGTTCCAAGTGCAGGCTCCAGCCCGGGGAACTCTGGGAAGAGTGTACCCAGGC
AGCAGGAGCTCAGAAAAGCACAGCCCTGACAGTGCCTGCTCTGTGGATTACAGCAGCAGCTGCCTTTCCA
GCCCGGAGCACCCCACTGAAGACTCTGAGAGCACGGAGCCCCTCAGTGTGGATGGCATCTCCTCAGACCT
TGAAGAGCCAGCTGAGGGTGATGAAGAAGAGGAAGAAGAGGAGGGAGGCATGGGCCCCTATGGGCTACAG
GAGGGCAGCCCCCAGACTCCAGACCAGGAGCAGTTTCTAAAACAGCACTTTGAGACTCTGGCCAGTGGAG
CTGCTCCAGGGGCCCCAGTGCAGGTCCCAGAGAGGTCAGAGTCTCGGAGTATCTCTTCACGATTCCTGTT
GCAAGTACAGACCCGCCCACTCAGGGAACCATCCCCATCCTCCTCAAGCCTGGCACTGATGTCGAGACCA
GCCCAGGTGCCACAGGCATCTGGTGAGCAGCCGAGAGGCAATGGTGCCAATCCCCCTGGAGCACCCCCGG
AGGTGGAACCGTCCTCTGGCAACCCCAGCCCCCAGCAGGCAGCCTCTGTGCTGTTGCCACGATGCCGTCT
CAACCCTGACAGCAGCTGGGCTCCCAAGAGAGTGGCCACAGCCAGCCCCTTTTCTGGACTCCAGAAGGCC
CAGTCTGTGCACAGTCTGGTGCCACAGGAAAGACATGAGGCCAGTCTGCAGGCCCCTTCACCAGGCGCAC
TGCTGTCTCGGGAGATTGAAGCTCAGGATGGTCTGGGCTCCCTGCCCCCAGCTGATGGCCGTCCGTCTCG
GCCTCACTCCTATCAGAACCCCACCACCAGTTCCATGGCCAAGATATCCCGCAGTATCTCTGTTGGGGAG
AACCTGGGCCTGGTGGCTGAACCTCAAGCTCATGCCCCCATCCGAGTCTCACCACTCAGCAAGCTGGCCC
TGCCCAGCCGGGCTCACCTGGTCCTGGACATCCCCAAACCACTGCCTGACCGTCCTACCCTGGCTGCATT
CTCTCCTGTCACCAAAGGCCGGGCCCCTGGCGAGGCAGAAAAGCCTGGCTTCCCGGTGGGCCTAGGAAAA
GCTCACAGTACAACTGAGAGATGGGCCTGTTTGGGGGAGGGCACCACTCCCAAGCCTAGGACAGAGTGCC
AGGCTCATCCTGGGCCCAGCAGCCCCTGTGCCCAGCAACTGCCAGTCAGCAGCCTCTTCCAAGGCCCTGA
AAACTTGCAGCCCCCACCCCCTGAGAAGACTCCCAACCCCATGGAATGCACCAAGCCAGGGGCAGCCCTG
AGCCAGGACTCAGAGCCAGCGGTGAGCCTGGAGCAGTGTGAGCAGCTGGTGGCAGAGCTCCGCGGCAGCG
TGCGCCAGGCAGTGCGGCTCTACCACTCGGTGGCTGGCTGCAAGATGCCCTCAGCAGAGCAAAGTCGGAT
TGCCCAGCTCCTCAGAGACACCTTCTCTTCAGTGCGACAGGAGCTGGAAGCTGTGGCTGGGGCAGTGCTG
TCCAGCCCAGGCAGCAGCCCTGGGGCTGTGGGAGCCGAGCAGACACAGGCCCTGCTGGAGCAATACTCAG
AACTGTTGCTTCGAGCCGTGGAACGGCGTATGGAACGCAAACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221849 protein sequence
Red=Cloning site Green=Tags(s)

MAVEGSTITSRIKNLLRSPSIKLRRSKAGNRREDLSSKVTLEKVLGITVSGGRGLACDPRSGLVAYPAGC
VVVLFNPRKHKQHHILNSSRKTITALAFSPDGKYLVTGESGHMPAVRVWDVAEHSQVAELQEHKYGVACV
AFSPSAKYIVSVGYQHDMIVNVWAWKKNIVVASNKVSSRVTAVSFSEDCSYFVTAGNRHIKFWYLDDSKT
SKVNATVPLLGRSGLLGELRNNLFTDVACGRGKKADSTFCITSSGLLCEFSDRRLLDKWVELRTTVAHCI
SVSQDYIFCGCADGTVRLFNPSNLHFLSTLPRPHALGTDIASVTEASRLFSGVANARYPDTIALTFDPTN
QWLSCVYNDHSIYVWDVRDPKKVGKVYSALYHSSCVWSVEVYPEVKDSNQACLPPSSFITCSSDNTIRLW
NTESSGVHGSTLHRNILSSDLIKIIYVDGNTQALLDTELPGGDKADASLLDPRVGIRSVCVSPNGQHLAS
GDRMGTLRVHELQSLSEMLKVEAHDSEILCLEYSKPDTGLKLLASASRDRLIHVLDAGREYSLQQTLDEH
SSSITAVKFAASDGQVRMISCGADKSIYFRTAQKSGDGVQFTRTHHVVRKTTLYDMDVEPSWKYTAIGCQ
DRNIRIFNISSGKQKKLFKGSQGEDGTLIKVQTDPSGIYIATSCSDKNLSIFDFSSGECVATMFGHSEIV
TGMKFSNDCKHLISVSGDSCIFVWRLSSEMTISMRQRLAELRQRQRGGKQQGPSSPQRASGPNRHQAPSM
LSPGPALSSDSDKEGEDEGTEEELPALPVLAKSTKKALASVPSPALPRSLSHWEMSRAQESVGFLDPAPA
ANPGPRRRGRWVQPGVELSVRSMLDLRQLETLAPSLQDPSQDSLAIIPSGPRKHGQEALETSLTSQNEKP
PRPQASQPCSYPHIIRLLSQEEGVFAQDLEPAPIEDGIVYPEPSDNPTMDTSEFQVQAPARGTLGRVYPG
SRSSEKHSPDSACSVDYSSSCLSSPEHPTEDSESTEPLSVDGISSDLEEPAEGDEEEEEEEGGMGPYGLQ
EGSPQTPDQEQFLKQHFETLASGAAPGAPVQVPERSESRSISSRFLLQVQTRPLREPSPSSSSLALMSRP
AQVPQASGEQPRGNGANPPGAPPEVEPSSGNPSPQQAASVLLPRCRLNPDSSWAPKRVATASPFSGLQKA
QSVHSLVPQERHEASLQAPSPGALLSREIEAQDGLGSLPPADGRPSRPHSYQNPTTSSMAKISRSISVGE
NLGLVAEPQAHAPIRVSPLSKLALPSRAHLVLDIPKPLPDRPTLAAFSPVTKGRAPGEAEKPGFPVGLGK
AHSTTERWACLGEGTTPKPRTECQAHPGPSSPCAQQLPVSSLFQGPENLQPPPPEKTPNPMECTKPGAAL
SQDSEPAVSLEQCEQLVAELRGSVRQAVRLYHSVAGCKMPSAEQSRIAQLLRDTFSSVRQELEAVAGAVL
SSPGSSPGAVGAEQTQALLEQYSELLLRAVERRMERKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_014994
ORF Size 4524 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014994.2, NP_055809.2
RefSeq Size 7174 bp
RefSeq ORF 4527 bp
Locus ID 23005
UniProt ID O60336
Cytogenetics 15q15.1
Protein Families Druggable Genome
MW 163 kDa
Summary This gene encodes a scaffold protein that regulates the JNK (c-Jun N-terminal kinase) and NOD2 (nucleotide-binding oligomerization domain-containing protein 2) signaling pathways. The encoded protein interacts with another related JNK pathway scaffold protein, WDR62, via a conserved dimerization domain, and enhances JNK signaling. This protein may play a role in bacterial immunity by binding to the NOD2 receptor and negatively regulating downstream antibacterial and pro-inflammatory signaling. Mutations in this gene that impair cellular localization of the encoded protein cause a form of nephronophthisis, an autosomal-recessive kidney disorder, in human patients. provided by RefSeq, May 2017
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"NM_014994" in other vectors (6)
SKU Description Size Price
RC221849L1 Lenti-ORF clone of MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L2 Lenti-ORF clone of MAPKBP1 (mGFP-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L3 Lenti-ORF clone of MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L4 Lenti-ORF clone of MAPKBP1 (mGFP-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RG221849 MAPKBP1 (tGFP-tagged) - Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,490.00
SC304199 MAPKBP1 (untagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,420.00

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