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MAPKBP1 (NM_014994) Human Untagged Clone

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SKU
SC304199
MAPKBP1 (untagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1
  $1,420.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAPKBP1
Synonyms JNKBP-1; JNKBP1; NPHP20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304199 representing NM_014994.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTGTGGAAGGGTCAACCATTACCAGCCGGATCAAGAATCTGTTGAGATCTCCATCCATCAAACTG
CGCAGGAGTAAGGCAGGAAACCGACGAGAGGACCTCAGCTCCAAGGTGACCTTGGAGAAGGTGCTGGGA
ATTACAGTGTCTGGAGGCAGAGGACTTGCCTGTGACCCCCGATCAGGTTTAGTTGCTTACCCAGCAGGG
TGTGTGGTTGTGTTGTTCAATCCCCGGAAACACAAACAGCACCACATCCTCAACAGTTCCAGGAAAACC
ATCACTGCCCTTGCCTTCTCCCCTGATGGCAAGTACTTGGTCACTGGAGAGAGTGGGCACATGCCTGCC
GTGCGGGTTTGGGACGTGGCAGAGCACAGCCAGGTGGCCGAGCTGCAGGAGCACAAGTATGGTGTGGCT
TGTGTGGCCTTCTCTCCTAGCGCCAAGTACATTGTCTCTGTGGGCTACCAGCATGACATGATCGTCAAC
GTGTGGGCCTGGAAGAAAAACATTGTGGTGGCCTCCAACAAGGTGTCCAGTCGGGTGACAGCAGTGTCC
TTCTCTGAGGATTGCAGCTACTTTGTCACTGCAGGCAACCGACACATCAAATTCTGGTATCTCGATGAC
AGCAAGACCTCAAAGGTGAATGCCACTGTGCCCTTGCTGGGCCGCTCAGGGCTGCTGGGAGAGCTACGG
AACAACCTATTCACTGATGTGGCCTGTGGCAGAGGAAAAAAGGCGGACAGTACCTTCTGCATCACGTCC
TCAGGGCTGCTGTGCGAGTTCAGTGATCGAAGGCTTTTGGACAAGTGGGTGGAGCTGAGGACCACAGTG
GCCCACTGCATCTCTGTGAGCCAAGACTACATCTTCTGTGGCTGTGCTGATGGCACCGTGCGCCTTTTC
AACCCCTCTAACCTGCACTTCCTTAGCACCTTGCCCCGACCCCATGCTCTGGGGACAGACATTGCTAGC
GTCACCGAGGCCAGTCGCCTCTTCTCTGGAGTGGCGAATGCCAGGTATCCAGACACCATTGCCTTGACC
TTTGATCCTACTAATCAGTGGCTGTCTTGTGTGTACAACGATCATAGCATTTATGTTTGGGATGTGAGG
GACCCCAAGAAAGTGGGCAAGGTGTACTCGGCTCTGTATCATTCTTCCTGCGTCTGGAGTGTGGAGGTC
TACCCCGAGGTGAAGGATAGTAACCAGGCCTGCCTGCCCCCCAGTTCCTTTATTACCTGCTCCTCAGAC
AACACCATCCGCCTGTGGAACACAGAGAGCTCCGGGGTGCATGGCTCCACCCTCCACCGAAACATCCTC
AGCAGTGACCTCATTAAAATCATCTATGTGGATGGGAACACCCAGGCCCTGCTGGACACAGAGCTGCCT
GGAGGAGACAAAGCTGATGCATCCCTGTTGGATCCCCGCGTGGGCATCCGCTCGGTGTGTGTCAGCCCC
AATGGACAGCATCTAGCATCAGGGGACCGTATGGGCACACTTAGGGTGCACGAACTTCAGTCCCTGAGT
GAGATGCTGAAGGTGGAGGCCCATGACTCTGAGATTCTGTGCCTGGAGTATTCTAAGCCAGACACAGGT
CTGAAACTGCTAGCATCGGCGAGCCGGGACCGGCTGATCCATGTGCTGGATGCCGGGCGGGAGTACAGC
CTACAGCAGACGCTGGACGAACACTCATCCTCCATCACTGCTGTTAAGTTTGCAGCCAGTGATGGGCAA
GTCCGCATGATCAGCTGTGGAGCAGACAAGAGCATCTACTTCCGCACTGCGCAGAAGTCTGGAGATGGA
GTGCAGTTCACACGGACACACCACGTGGTGCGGAAGACGACCCTCTATGACATGGATGTGGAGCCCAGC
TGGAAGTACACGGCTATCGGCTGCCAGGACCGAAATATTCGGATATTTAACATCAGCAGTGGAAAGCAG
AAGAAGCTGTTTAAAGGGTCACAGGGTGAGGACGGCACACTCATTAAGGTGCAGACAGACCCCTCAGGG
ATCTACATTGCCACCAGCTGTTCTGACAAGAATCTCTCCATTTTTGACTTCTCCTCAGGCGAGTGCGTG
GCCACCATGTTTGGCCACTCAGAGATTGTCACTGGCATGAAATTTAGTAATGATTGTAAACATCTCATC
TCTGTGTCTGGGGACAGCTGCATATTTGTGTGGCGCCTGAGCTCTGAGATGACCATCAGCATGAGGCAG
CGTCTGGCCGAGTTGCGCCAGCGTCAGCGGGGCGGCAAGCAGCAAGGACCATCCTCTCCCCAAAGGGCT
TCTGGACCCAACCGGCACCAGGCCCCATCAATGCTGTCTCCTGGACCGGCTCTCTCATCAGACAGTGAC
AAGGAGGGAGAAGATGAGGGGACTGAAGAAGAACTTCCAGCACTGCCCGTCCTTGCCAAGAGTACCAAG
AAGGCACTGGCCTCGGTCCCCAGCCCAGCTTTGCCCCGAAGCCTGTCCCACTGGGAGATGAGTCGGGCA
CAGGAGTCCGTGGGGTTCCTGGACCCAGCTCCTGCAGCCAACCCAGGACCCAGAAGAAGAGGGCGCTGG
GTTCAGCCAGGTGTGGAACTGAGCGTTAGATCCATGCTGGATCTGCGGCAGCTGGAAACACTGGCCCCA
AGCCTGCAGGACCCTAGCCAGGACTCGCTGGCCATCATCCCATCTGGTCCCAGGAAGCATGGGCAGGAG
GCCCTTGAGACTTCACTCACTAGCCAGAATGAAAAGCCCCCTCGGCCTCAGGCTTCCCAACCTTGTTCC
TATCCCCATATTATCCGATTATTGTCACAAGAGGAAGGGGTCTTTGCCCAAGATCTGGAACCTGCACCC
ATTGAAGATGGTATTGTCTACCCGGAGCCGAGTGACAACCCCACCATGGATACCAGTGAGTTCCAAGTG
CAGGCTCCAGCCCGGGGAACTCTGGGAAGAGTGTACCCAGGCAGCAGGAGCTCAGAAAAGCACAGCCCT
GACAGTGCCTGCTCTGTGGATTACAGCAGCAGCTGCCTTTCCAGCCCGGAGCACCCCACTGAAGACTCT
GAGAGCACGGAGCCCCTCAGTGTGGATGGCATCTCCTCAGACCTTGAAGAGCCAGCTGAGGGTGATGAA
GAAGAGGAAGAAGAGGAGGGAGGCATGGGCCCCTATGGGCTACAGGAGGGCAGCCCCCAGACTCCAGAC
CAGGAGCAGTTTCTAAAACAGCACTTTGAGACTCTGGCCAGTGGAGCTGCTCCAGGGGCCCCAGTGCAG
GTCCCAGAGAGGTCAGAGTCTCGGAGTATCTCTTCACGATTCCTGTTGCAAGTACAGACCCGCCCACTC
AGGGAACCATCCCCATCCTCCTCAAGCCTGGCACTGATGTCGAGACCAGCCCAGGTGCCACAGGCATCT
GGTGAGCAGCCGAGAGGCAATGGTGCCAATCCCCCTGGAGCACCCCCGGAGGTGGAACCGTCCTCTGGC
AACCCCAGCCCCCAGCAGGCAGCCTCTGTGCTGTTGCCACGATGCCGTCTCAACCCTGACAGCAGCTGG
GCTCCCAAGAGAGTGGCCACAGCCAGCCCCTTTTCTGGACTCCAGAAGGCCCAGTCTGTGCACAGTCTG
GTGCCACAGGAAAGACATGAGGCCAGTCTGCAGGCCCCTTCACCAGGCGCACTGCTGTCTCGGGAGATC
GAAGCTCAGGATGGTCTGGGCTCCCTGCCCCCAGCTGATGGCCGTCCGTCTCGGCCTCACTCCTATCAG
AACCCCACCACCAGTTCCATGGCCAAGATATCCCGCAGTATCTCTGTTGGGGAGAACCTGGGCCTGGTG
GCTGAACCTCAAGCTCATGCCCCCATCCGAGTCTCACCACTCAGCAAGCTGGCCCTGCCCAGCCGGGCT
CACCTGGTCCTGGACATCCCCAAACCACTGCCTGACCGTCCTACCCTGGCTGCATTCTCTCCTGTCACC
AAAGGCCGGGCCCCTGGCGAGGCAGAAAAGCCTGGCTTCCCGGTGGGCCTAGGAAAAGCTCACAGTACA
ACTGAGAGATGGGCCTGTTTGGGGGAGGGCACCACTCCCAAGCCTAGGACAGAGTGCCAGGCTCATCCT
GGGCCCAGCAGCCCCTGTGCCCAGCAACTGCCAGTCAGCAGCCTCTTCCAAGGCCCTGAAAACTTGCAG
CCCCCACCCCCTGAGAAGACTCCCAACCCCATGGAATGCACCAAGCCAGGGGCAGCCCTGAGCCAGGAC
TCAGAGCCAGCGGTGAGCCTGGAGCAGTGTGAGCAGCTGGTGGCAGAGCTCCGCGGCAGCGTGCGCCAG
GCAGTGCGGCTCTACCACTCGGTGGCTGGCTGCAAGATGCCCTCAGCAGAGCAAAGTCGGATTGCCCAG
CTCCTCAGAGACACCTTCTCTTCAGTGCGACAGGAGCTGGAAGCTGTGGCTGGGGCAGTGCTGTCCAGC
CCAGGCAGCAGCCCTGGGGCTGTGGGAGCCGAGCAGACACAGGCCCTGCTGGAGCAATACTCAGAACTG
TTGCTTCGAGCCGTGGAACGGCGTATGGAACGCAAACTCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_014994
Insert Size 4527 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014994.2
RefSeq Size 7247 bp
RefSeq ORF 4527 bp
Locus ID 23005
UniProt ID O60336
Cytogenetics 15q15.1
Protein Families Druggable Genome
MW 163.1 kDa
Summary This gene encodes a scaffold protein that regulates the JNK (c-Jun N-terminal kinase) and NOD2 (nucleotide-binding oligomerization domain-containing protein 2) signaling pathways. The encoded protein interacts with another related JNK pathway scaffold protein, WDR62, via a conserved dimerization domain, and enhances JNK signaling. This protein may play a role in bacterial immunity by binding to the NOD2 receptor and negatively regulating downstream antibacterial and pro-inflammatory signaling. Mutations in this gene that impair cellular localization of the encoded protein cause a form of nephronophthisis, an autosomal-recessive kidney disorder, in human patients. provided by RefSeq, May 2017
Transcript Variant: This variant (1) encodes isoform a.
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Documents
Resources
"NM_014994" in other vectors (6)
SKU Description Size Price
RC221849 MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,290.00
RC221849L1 Lenti-ORF clone of MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L2 Lenti-ORF clone of MAPKBP1 (mGFP-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L3 Lenti-ORF clone of MAPKBP1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RC221849L4 Lenti-ORF clone of MAPKBP1 (mGFP-tagged)-Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,590.00
RG221849 MAPKBP1 (tGFP-tagged) - Human mitogen-activated protein kinase binding protein 1 (MAPKBP1), transcript variant 1 10 ug
$1,490.00

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