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AP1S3 (NM_001039569) Human Tagged ORF Clone

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SKU
RC220329
AP1S3 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "AP1S3 or NM_001039569"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AP1S3
Synonyms PSORS15; sigma1C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220329 representing NM_001039569
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATACATTTCATATTGCTCTTCAGTCGACAAGGGAAATTACGGCTACAGAAATGGTACATCACTCTCC
CTGATAAAGAGAGGAAGAAGATCACCCGGGAAATTGTTCAGATTATTCTCTCCCGTGGTCACAGGACAAG
CAGTTTTGTTGACTGGAAGGAGCTAAAACTTGTTTATAAAAGGTATGCTAGTTTATATTTTTGCTGTGCA
ATAGAAAATCAGGACAATGAGCTCTTGACGCTAGAGATTGTGCATCGTTACGTGGAGCTGCTGGACAAAT
ATTTTGGAAATGTCTGTGAGCTGGATATTATCTTTAATTTTGAAAAGGCTTATTTCATCCTGGACGAGTT
TATAATAGGTGGGGAAATTCAGGAAACATCCAAGAAAATTGCTGTCAAAGCCATTGAAGACTCTGATATG
TTACAGGAGACAATGGAAGAATACATGAACAAGCCTACATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220329 representing NM_001039569
Red=Cloning site Green=Tags(s)

MIHFILLFSRQGKLRLQKWYITLPDKERKKITREIVQIILSRGHRTSSFVDWKELKLVYKRYASLYFCCA
IENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIGGEIQETSKKIAVKAIEDSDM
LQETMEEYMNKPTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039569
ORF Size 462 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039569.2
RefSeq Size 4005 bp
RefSeq ORF 465 bp
Locus ID 130340
UniProt ID Q96PC3
Cytogenetics 2q36.1
Protein Pathways Lysosome
MW 18.3 kDa
Summary This gene encodes a member of the adaptor-related protein complex 1, sigma subunit genes. The encoded protein is a component of adaptor protein complex 1 (AP-1), one of the AP complexes involved in claathrin-mediated vesicular transport from the Golgi or endosomes. Disruption of the pathway for display of HIV-1 antigens, which prevents recognition of the virus by cytotoxic T cells, has been shown to involve the AP-1 complex (PMID: 15569716). Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
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"NM_001039569" in other vectors (4)
SKU Description Size Price
RC220329L3 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3), Myc-DDK-tagged 10 ug
$450.00
RC220329L4 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3), mGFP tagged 10 ug
$450.00
RG220329 AP1S3 (tGFP-tagged) - Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3) 10 ug
$350.00
SC310773 AP1S3 (untagged)-Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3) 10 ug
$165.00

Plasmid Map for RC220329

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220329
Circular map for RC220329

file-alt Citations

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