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AP1S3 (NM_001039569) Human Untagged Clone

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SKU
SC310773
AP1S3 (untagged)-Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3)
  $165.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol AP1S3
Synonyms PSORS15; sigma1C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC310773 representing NM_001039569.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATACATTTCATATTGCTCTTCAGTCGACAAGGGAAATTACGGCTACAGAAATGGTACATCACTCTC
CCTGATAAAGAGAGGAAGAAGATCACCCGGGAAATTGTTCAGATTATTCTCTCCCGTGGTCACAGGACA
AGCAGTTTTGTTGACTGGAAGGAGCTAAAACTTGTTTATAAAAGGTATGCTAGTTTATATTTTTGCTGT
GCAATAGAAAATCAGGACAATGAGCTCTTGACGCTAGAGATTGTGCATCGTTACGTGGAGCTGCTGGAC
AAATATTTTGGAAATGTCTGTGAGCTGGATATTATCTTTAATTTTGAAAAGGCTTATTTCATCCTGGAC
GAGTTTATAATAGGTGGGGAAATTCAGGAAACATCCAAGAAAATTGCTGTCAAAGCCATTGAAGACTCT
GATATGTTACAGGAGACAATGGAAGAATACATGAACAAGCCTACATTTTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001039569
Insert Size 465 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039569.1
RefSeq Size 4005 bp
RefSeq ORF 465 bp
Locus ID 130340
UniProt ID Q96PC3
Cytogenetics 2q36.1
Protein Pathways Lysosome
MW 18.5 kDa
Summary This gene encodes a member of the adaptor-related protein complex 1, sigma subunit genes. The encoded protein is a component of adaptor protein complex 1 (AP-1), one of the AP complexes involved in claathrin-mediated vesicular transport from the Golgi or endosomes. Disruption of the pathway for display of HIV-1 antigens, which prevents recognition of the virus by cytotoxic T cells, has been shown to involve the AP-1 complex (PMID: 15569716). Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001039569" in other vectors (4)
SKU Description Size Price
RC220329 AP1S3 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3) 10 ug
$150.00
RC220329L3 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3), Myc-DDK-tagged 10 ug
$450.00
RC220329L4 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3), mGFP tagged 10 ug
$450.00
RG220329 AP1S3 (tGFP-tagged) - Human adaptor-related protein complex 1, sigma 3 subunit (AP1S3) 10 ug
$350.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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