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TCF12 (NM_207036) Human Tagged ORF Clone

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SKU
RC220164
TCF12 (Myc-DDK-tagged)-Human transcription factor 12 (TCF12), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $649.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TCF12
Synonyms bHLHb20; CRS3; HEB; HsT17266; HTF4; p64; TCF-12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220164 representing NM_207036
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCCCAGCAACAACGCATGGCCGCTATAGGGACCGACAAGGAGCTGAGCGACCTACTGGACTTCA
GTGCGATGTTTTCCCCACCTGTTAATAGTGGGAAAACTAGACCAACTACACTGGGAAGCAGTCAATTCAG
TGGATCAGGTATTGATGAAAGAGGAGGTACAACATCTTGGGGAACAAGTGGTCAACCAAGTCCTTCCTAT
GATTCATCTAGAGGTTTTACAGACAGCCCTCATTACAGTGATCACTTGAATGACAGTCGATTAGGAGCCC
ATGAAGGCTTGTCCCCAACACCTTTCATGAACTCAAATCTGATGGGAAAAACATCAGAGAGAGGCTCATT
TTCCCTGTACAGCAGAGATACTGGATTACCAGGCTGTCAATCTAGTCTCCTGAGACAAGATCTGGGGCTT
GGGAGCCCAGCACAGCTATCTTCTTCAGGAAAACCTGGGACAGCATACTATTCATTCTCTGCTACAAGTT
CCAGGAGGAGACCACTCCATGACTCTGCAGCGCTTGATCCCTTGCAAGCAAAAAAAGTCAGAAAGGTGCC
TCCTGGTTTGCCTTCTTCTGTATATGCACCATCCCCAAATTCAGATGATTTCAACCGTGAATCTCCTAGT
TATCCATCTCCTAAGCCACCAACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAATT
CTTCTGACCTTTGGAGTTCATCAAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCAC
TTCCCACATGTCTCAATCCAGTAGTTATGGCAACCTTCATTCACATGACCGCTTGAGTTATCCTCCACAC
TCAGTTTCACCAACAGACATAAACACGAGTCTTCCACCAATGTCCAGCTTTCATCGCGGCAGTACCAGCA
GTTCACCTTACGTTGCTGCCTCACACACTCCTCCCATCAATGGATCAGACAGCATTCTAGGAACCAGAGG
GAATGCTGCTGGAAGCTCACAGACAGGTGATGCACTTGGAAAGGCTTTGGCATCTATTTATTCTCCTGAC
CATACCAGCAGTAGTTTTCCGTCAAATCCATCAACACCAGTTGGATCACCTTCACCTCTCACAGGTACCA
GTCAGTGGCCAAGACCTGGAGGGCAAGCACCTTCATCCCCAAGCTATGAAAACTCACTCCACTCCCTGAA
AAATCGAGTTGAGCAGCAACTTCACGAGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAG
CAGTCTCGAATGGAGGATCGTTTAGACAGACTGGATGATGCAATCCATGTGCTGCGGAACCATGCTGTGG
GACCTTCCACCAGTTTGCCTGCTGGTCACAGTGATATACATAGTTTATTGGGACCATCCCATAATGCACC
AATTGGAAGCCTCAATTCAAACTATGGAGGATCAAGCCTTGTTGCAAGCAGTCGATCAGCTTCAATGGTT
GGAACTCATCGGGAAGACTCTGTCAGTCTCAATGGCAATCATTCAGTCCTGTCTAGTACAGTCACTACTT
CAAGCACAGACCTGAACCATAAAACACAAGAAAATTATAGAGGTGGCTTGCAAAGTCAGTCTGGAACTGT
TGTTACAACAGAAATCAAGACTGAAAACAAAGAAAAGGATGAAAACCTTCATGAACCTCCTTCATCAGAT
GACATGAAGTCAGATGATGAATCCTCCCAAAAAGATATCAAGGTTTCATCTAGAGGCAGAACAAGCAGTA
CTAATGAAGATGAGGATTTGAACCCTGAACAGAAGATAGAAAGGGAGAAGGAGAGGCGGATGGCTAACAA
TGCCAGAGAACGCTTACGCGTGCGGGATATTAATGAAGCATTCAAAGAGCTTGGCCGAATGTGTCAGCTT
CACTTGAAGAGTGAAAAACCCCAAACAAAACTCCTTATTCTTCATCAAGCCGTGGCAGTCATCCTTAGTC
TAGAACAGCAAGTCAGAGAGAGGAACCTTAACCCCAAAGCAGCCTGCCTTAAGAGAAGGGAAGAAGAAAA
AGTTTCTGCCGTATCGGCAGAGCCGCCAACCACACTGCCAGGAACCCATCCTGGGCTTAGTGAAACTACC
AACCCTATGGGTCATATGAGCGGACCG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220164 representing NM_207036
Red=Cloning site Green=Tags(s)

MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGIDERGGTTSWGTSGQPSPSY
DSSRGFTDSPHYSDHLNDSRLGAHEGLSPTPFMNSNLMGKTSERGSFSLYSRDTGLPGCQSSLLRQDLGL
GSPAQLSSSGKPGTAYYSFSATSSRRRPLHDSAALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPS
YPSPKPPTSMFASTFFMQDGTHNSSDLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGNLHSHDRLSYPPH
SVSPTDINTSLPPMSSFHRGSTSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPD
HTSSSFPSNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCE
QSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMV
GTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSD
DMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQL
HLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETT
NPMGHMSGP

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_207036
ORF Size 2127 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207036.1, NP_996919.1
RefSeq Size 4796 bp
RefSeq ORF 2121 bp
Locus ID 6938
UniProt ID Q99081, A0A024R5Z0
Cytogenetics 15q21.3
Protein Families Druggable Genome, Transcription Factors
MW 75.7 kDa
Summary The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. provided by RefSeq, Jul 2008
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Documents
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"NM_207036" in other vectors (6)
SKU Description Size Price
RC220164L1 Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, Myc-DDK-tagged 10 ug
$949.00
RC220164L2 Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, mGFP tagged 10 ug
$949.00
RC220164L3 Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, Myc-DDK-tagged 10 ug
$949.00
RC220164L4 Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, mGFP tagged 10 ug
$949.00
RG220164 TCF12 (tGFP-tagged) - Human transcription factor 12 (TCF12), transcript variant 1 10 ug
$789.00 $849.00
SC308371 TCF12 (untagged)-Human transcription factor 12 (TCF12), transcript variant 1 10 ug
$647.00

Plasmid Map for RC220164

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220164
Circular map for RC220164

file-alt Citations

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