TCF12 (NM_207036) Human Untagged Clone
$647.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | TCF12 |
| Synonyms | bHLHb20; CRS3; HEB; HsT17266; HTF4; p64; TCF-12 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_207036 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_207036.1, NP_996919.1 |
| RefSeq Size | 4796 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 6938 |
| UniProt ID | Q99081 |
| Cytogenetics | 15q21.3 |
| Protein Families | Druggable Genome, Transcription Factors |
| Summary | The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 2 both encode the same isoform a. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_207036" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220164 | TCF12 (Myc-DDK-tagged)-Human transcription factor 12 (TCF12), transcript variant 1 | 10 ug |
$649.00
|
|
| RC220164L1 | Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, Myc-DDK-tagged | 10 ug |
$949.00
|
|
| RC220164L2 | Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, mGFP tagged | 10 ug |
$949.00
|
|
| RC220164L3 | Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, Myc-DDK-tagged | 10 ug |
$949.00
|
|
| RC220164L4 | Lenti ORF clone of Human transcription factor 12 (TCF12), transcript variant 1, mGFP tagged | 10 ug |
$949.00
|
|
| RG220164 | TCF12 (tGFP-tagged) - Human transcription factor 12 (TCF12), transcript variant 1 | 10 ug |
$789.00
$849.00
|
|
Citations
Mutations in TCF12, encoding a basic helix-loop-helix partner of TWIST1, are a frequent cause of coronal craniosynostosis
Sharma, Fenwick, Brockop et al
Nat. Genet. (2013) 45 (3), 304-7 DOI: 10.1038/ng.2531
Sharma, Fenwick, Brockop et al
Nat. Genet. (2013) 45 (3), 304-7 DOI: 10.1038/ng.2531
Cited by 228
AI Summary: OriGene’s full-length TCF12 cDNA clone (pCMV6-XL5) was used to study the functional effects of TCF12 mutations in transactivation assays, helping to demonstrate the critical interaction between TCF12 and TWIST1 in coronal craniosynostosis.
Snippet: by ELISA. Transactivation analysis of missense mutations. We obtained a full-length TCF12 cDNA clone (pCMV6-XL5, encoding HEBβ) from OriGene and synthesized the TWIST1" style="font-weight: bold;">TWIST1 clone (pCMV-Twist) in house. The pCaSpeR
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
