Cathepsin E (CTSE) (NM_148964) Human Tagged ORF Clone

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SKU
RC220117
CTSE (Myc-DDK-tagged)-Human cathepsin E (CTSE), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Cathepsin E
Synonyms CATE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220117 representing NM_148964
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAACGCTCCTTCTTTTGCTGCTGGTGCTCCTGGAGCTGGGAGAGGCCCAAGGATCCCTTCACAGGG
TGCCCCTCAGGAGGCATCCGTCCCTCAAGAAGAAGCTGCGGGCACGGAGCCAGCTCTCTGAGTTCTGGAA
ATCCCATAATTTGGACATGATCCAGTTCACCGAGTCCTGCTCAATGGACCAGAGTGCCAAGGAACCCCTC
ATCAACTACTTGGATATGGAATACTTCGGCACTATCTCCATTGGCTCCCCACCACAGAACTTCACTGTCA
TCTTCGACACTGGCTCCTCCAACCTCTGGGTCCCCTCTGTGTACTGCACTAGCCCAGCCTGCAAGACGCA
CAGCAGGTTCCAGCCTTCCCAGTCCAGCACATACAGCCAGCCAGGTCAATCTTTCTCCATTCAGTATGGA
ACCGGGAGCTTGTCCGGGATCATTGGAGCCGACCAAGTCTCTGTGGAAGGACTAACCGTGGTTGGCCAGC
AGTTTGGAGAAAGTGTCACAGAGCCAGGCCAGACCTTTGTGGATGCAGAGTTTGATGGAATTCTGGGCCT
GGGATACCCCTCCTTGGCTGTGGGAGGAGTGACTCCAGTATTTGACAACATGATGGCTCAGAACCTGGTG
GACTTGCCGATGTTTTCTGTCTACATGAGCAGTAACCCAGAAGGTGGTGCGGGGAGCGAGCTGATTTTTG
GAGGCTACGACCACTCCCATTTCTCTGGGAGCCTGAATTGGGTCCCAGTCACCAAGCAAGCTTACTGGCA
GATTGCACTGGATAATATGCTGTGGAGTGTGCCAACCTTAACGTCATGCCGGATGTCACCTTCACCATTA
ACGGAGTCCCCTATACCCTCAGCCCAACTGCCTACACCCTACTGGACTTCGTGGATGGAATGCAGTTCTG
CAGCAGTGGCTTTCAAGGACTTGACATCCACCCTCCAGCTGGGCCCCTCTGGATCCTGGGGGATGTCTTC
ATTCGACAGTTTTACTCAGTCTTTGACCGTGGGAATAACCGTGTGGGACTGGCCCCAGCAGTCCCCTAAG
GAGGGGCCTTGTGTCTGTGCCTGCCTGTCTGACAGACCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC220117 representing NM_148964
Red=Cloning site Green=Tags(s)

MKTLLLLLLVLLELGEAQGSLHRVPLRRHPSLKKKLRARSQLSEFWKSHNLDMIQFTESCSMDQSAKEPL
INYLDMEYFGTISIGSPPQNFTVIFDTGSSNLWVPSVYCTSPACKTHSRFQPSQSSTYSQPGQSFSIQYG
TGSLSGIIGADQVSVEGLTVVGQQFGESVTEPGQTFVDAEFDGILGLGYPSLAVGGVTPVFDNMMAQNLV
DLPMFSVYMSSNPEGGAGSELIFGGYDHSHFSGSLNWVPVTKQAYWQIALDNMLWSVPTLTSCRMSPSPL
TESPIPSAQLPTPYWTSWMECSSAAVAFKDLTSTLQLGPSGSWGMSSFDSFTQSLTVGITVWDWPQQSPK
EGPCVCACLSDRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148964
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148964.3
RefSeq Size 2086 bp
RefSeq ORF 1092 bp
Locus ID 1510
UniProt ID P14091
Cytogenetics 1q32.1
Protein Families Druggable Genome, Protease
Protein Pathways Lysosome
MW 37.7 kDa
Summary This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_148964" in other vectors (4)
SKU Description Size Price
RC220117L3 Lenti ORF clone of Human cathepsin E (CTSE), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC220117L4 Lenti ORF clone of Human cathepsin E (CTSE), transcript variant 2, mGFP tagged 10 ug
$757.00
RG220117 CTSE (tGFP-tagged) - Human cathepsin E (CTSE), transcript variant 2 10 ug
$657.00
SC309487 CTSE (untagged)-Human cathepsin E (CTSE), transcript variant 2 10 ug
$503.00

Plasmid Map for RC220117

Download sequence for

Cloning scheme for RC220117

Circular map for RC220117

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