PRODH (NM_016335) Human Tagged ORF Clone

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SKU
RC220096
PRODH (Myc-DDK-tagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1
  $837.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRODH
Synonyms HSPOX2; PIG6; POX; PRODH1; PRODH2; TP53I6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220096 representing NM_016335
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGAGGCGCGCCCTGCCCGCGCTGCGCCCCTGCATTCCCCGCTTCGTCCCGCTGTCCACGGCGC
CGGCCTCCCGCGAGCAGCCCGCAGCGGGCCCAGCGGCCGTGCCAGGAGGTGGGTCGGCCACGGCAGTGCG
GCCGCCGGTGCCCGCCGTGGACTTCGGCAACGCGCAGGAGGCGTACCGCAGCCGGCGAACCTGGGAGCTG
GCGCGGAGCCTGCTGGTGCTGCGCTTGTGCGCCTGGCCCGCGCTGCTGGCGCGCCACGAGCAGCTGCTGT
ATGTTTCCAGGAAACTTCTAGGACAGAGGCTATTCAACAAGCTCATGAAGATGACCTTCTATGGGCATTT
TGTAGCCGGGGAGGACCAGGAGTCCATCCAGCCCCTGCTTCGGCACTACAGGGCCTTCGGTGTCAGCGCC
ATCCTGGACTATGGAGTGGAGGAGGACCTGAGCCCCGAGGAGGCAGAGCACAAGGAGATGGAGTCCTGCA
CCTCAGCTGCGGAGAGGGATGGCAGTGGCACGAATAAGCGGGACAAGCAATACCAGGCCCACCGGGCCTT
CGGGGACCGCAGGAATGGTGTCATCAGTGCCCGCACCTACTTCTACGCCAATGAGGCCAAGTGCGACAGC
CACATGGAGACATTCTTGCGCTGCATCGAAGCCTCAGGTAGAGTCAGCGATGACGGCTTCATAGCCATTA
AGCTCACAGCACTGGGGAGACCCCAGTTTCTGCTGCAGTTCTCAGAGGTGCTGGCCAAGTGGAGGTGCTT
CTTTCACCAAATGGCTGTGGAGCAAGGGCAGGCGGGCCTGGCTGCCATGGACACCAAGCTGGAGGTGGCG
GTGCTGCAGGAAAGTGTCGCAAAGTTGGGCATCGCATCCAGGGCTGAGATTGAGGACTGGTTCACGGCAG
AGACCCTGGGAGTGTCTGGCACCATGGACCTGCTGGACTGGAGCAGCCTCATCGACAGCAGGACCAAGCT
GTCCAAGCACTTGGTAGTCCCCAACGCACAGACAGGACAGCTGGAGCCCCTGCTGTCCCGGTTCACTGAG
GAGGAGGAGCTACAGATGACCAGGATGCTACAGCGGATGGATGTCCTGGCCAAGAAAGCCACAGAGATGG
GCGTGCGGCTGATGGTGGATGCCGAGCAGACCTACTTCCAGCCGGCCATCAGCCGCCTGACGCTGGAGAT
GCAGCGGAAGTTCAATGTGGAGAAGCCGCTCATCTTCAACACATACCAGTGCTACCTCAAGGATGCCTAT
GACAATGTGACCCTGGACGTGGAGCTGGCTCGCCGTGAGGGCTGGTGTTTTGGGGCCAAGCTGGTGCGGG
GCGCATACCTGGCCCAGGAGCGAGCCCGTGCGGCAGAGATCGGCTATGAGGACCCCATCAACCCCACGTA
CGAGGCCACCAACGCCATGTACCACAGGTGCCTGGACTACGTGTTGGAGGAGCTGAAGCACAACGCCAAG
GCCAAGGTGATGGTGGCCTCCCACAATGAGGACACAGTGCGCTTTGCACTGCGCAGGATGGAGGAGCTGG
GCCTGCATCCTGCTGACCACCGGGTGTACTTTGGACAGCTGCTAGGCATGTGTGACCAGATCAGCTTCCC
GCTGGGCCAGGCTGGCTACCCCGTGTACAAGTACGTGCCCTATGGCCCCGTGATGGAGGTGCTGCCCTAC
TTGTCCCGCCGTGCCCTGGAGAACAGCAGCCTCATGAAGGGCACCCATCGGGAGCGGCAGCTGCTGTGGC
TGGAGCTCTTGAGGCGGCTCCGAACTGGCAACCTCTTCCATCGCCCTGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC220096 representing NM_016335
Red=Cloning site Green=Tags(s)

MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWEL
ARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSA
ILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHRAFGDRRNGVISARTYFYANEAKCDS
HMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVA
VLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTE
EEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAY
DNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAK
AKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPY
LSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016335
ORF Size 1800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016335.6
RefSeq Size 2400 bp
RefSeq ORF 1803 bp
Locus ID 5625
UniProt ID O43272
Cytogenetics 22q11.21
Protein Families Druggable Genome
Protein Pathways Arginine and proline metabolism, Metabolic pathways
MW 67.8 kDa
Summary This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Aug 2010
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"NM_016335" in other vectors (6)
SKU Description Size Price
RC220096L1 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$1,137.00
RC220096L2 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$1,137.00
RC220096L3 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$1,137.00
RC220096L4 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$1,137.00
RG220096 PRODH (tGFP-tagged) - Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$1,037.00
SC304405 PRODH (untagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$895.00

Plasmid Map for RC220096

Download sequence for

Cloning scheme for RC220096

Circular map for RC220096

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