PRODH (NM_016335) Human Untagged Clone

SKU
SC304405
PRODH (untagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1
  $895.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRODH
Synonyms HSPOX2; PIG6; POX; PRODH1; PRODH2; TP53I6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304405 representing NM_016335.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTCTGAGGCGCGCCCTGCCCGCGCTGCGCCCCTGCATTCCCCGCTTCGTCCCGCTGTCCACGGCG
CCGGCCTCCCGCGAGCAGCCCGCAGCGGGCCCAGCGGCCGTGCCAGGAGGTGGGTCGGCCACGGCAGTG
CGGCCGCCGGTGCCCGCCGTGGACTTCGGCAACGCGCAGGAGGCGTACCGCAGCCGGCGAACCTGGGAG
CTGGCGCGGAGCCTGCTGGTGCTGCGCTTGTGCGCCTGGCCCGCGCTGCTGGCGCGCCACGAGCAGCTG
CTGTATGTTTCCAGGAAACTTCTAGGACAGAGGCTATTCAACAAGCTCATGAAGATGACCTTCTATGGG
CATTTTGTAGCCGGGGAGGACCAGGAGTCCATCCAGCCCCTGCTTCGGCACTACAGGGCCTTCGGTGTC
AGCGCCATCCTGGACTATGGAGTGGAGGAGGACCTGAGCCCCGAGGAGGCAGAGCACAAGGAGATGGAG
TCCTGCACCTCAGCTGCGGAGAGGGATGGCAGTGGCACGAATAAGCGGGACAAGCAATACCAGGCCCAC
CGGGCCTTCGGGGACCGCAGGAATGGTGTCATCAGTGCCCGCACCTACTTCTACGCCAATGAGGCCAAG
TGCGACAGCCACATGGAGACATTCTTGCGCTGCATCGAAGCCTCAGGTAGAGTCAGCGATGACGGCTTC
ATAGCCATTAAGCTCACAGCACTGGGGAGACCCCAGTTTCTGCTGCAGTTCTCAGAGGTGCTGGCCAAG
TGGAGGTGCTTCTTTCACCAAATGGCTGTGGAGCAAGGGCAGGCGGGCCTGGCTGCCATGGACACCAAG
CTGGAGGTGGCGGTGCTGCAGGAAAGTGTCGCAAAGTTGGGCATCGCATCCAGGGCTGAGATTGAGGAC
TGGTTCACGGCAGAGACCCTGGGAGTGTCTGGCACCATGGACCTGCTGGACTGGAGCAGCCTCATCGAC
AGCAGGACCAAGCTGTCCAAGCACTTGGTAGTCCCCAACGCACAGACAGGACAGCTGGAGCCCCTGCTG
TCCCGGTTCACTGAGGAGGAGGAGCTACAGATGACCAGGATGCTACAGCGGATGGATGTCCTGGCCAAG
AAAGCCACAGAGATGGGCGTGCGGCTGATGGTGGATGCCGAGCAGACCTACTTCCAGCCGGCCATCAGC
CGCCTGACGCTGGAGATGCAGCGGAAGTTCAATGTGGAGAAGCCGCTCATCTTCAACACATACCAGTGC
TACCTCAAGGATGCCTATGACAATGTGACCCTGGACGTGGAGCTGGCTCGCCGTGAGGGCTGGTGTTTT
GGGGCCAAGCTGGTGCGGGGCGCATACCTGGCCCAGGAGCGAGCCCGTGCGGCAGAGATCGGCTATGAG
GACCCCATCAACCCCACGTACGAGGCCACCAACGCCATGTACCACAGGTGCCTGGACTACGTGTTGGAG
GAGCTGAAGCACAACGCCAAGGCCAAGGTGATGGTGGCCTCCCACAATGAGGACACAGTGCGCTTTGCA
CTGCGCAGGATGGAGGAGCTGGGCCTGCATCCTGCTGACCACCAGGTGTACTTTGGACAGCTGCTAGGC
ATGTGTGACCAGATCAGCTTCCCGCTGGGCCAGGCCGGCTACCCCGTGTACAAGTACGTGCCCTATGGC
CCCGTGATGGAGGTGCTGCCCTACTTGTCCCGCCGTGCCCTGGAGAACAGCAGCCTCATGAAGGGCACC
CATCGGGAGCGGCAGCTGCTGTGGCTGGAGCTCTTGAGGCGGCTCCGAACTGGCAACCTCTTCCATCGC
CCTGCCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_016335
Insert Size 1803 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016335.4
RefSeq Size 2423 bp
RefSeq ORF 1803 bp
Locus ID 5625
UniProt ID O43272
Cytogenetics 22q11.21
Protein Families Druggable Genome
Protein Pathways Arginine and proline metabolism, Metabolic pathways
MW 68 kDa
Summary This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Aug 2010
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_016335" in other vectors (6)
SKU Description Size Price
RC220096 PRODH (Myc-DDK-tagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$837.00
RC220096L1 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$1,137.00
RC220096L2 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$1,137.00
RC220096L3 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$1,137.00
RC220096L4 Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$1,137.00
RG220096 PRODH (tGFP-tagged) - Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$1,037.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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