Complement factor 8 beta (C8B) (NM_000066) Human Tagged ORF Clone

SKU RC219942

C8B (Myc-DDK-tagged)-Human complement component 8, beta polypeptide (C8B)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Complement factor 8 beta
• Synonyms: C82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219942 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCT; GTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTT; TGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGG; TCCTCTTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTC; AGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAG; AAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC; AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGC; ATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGA; GGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTT; AGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGT; ATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGG; TTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG; AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAAGTAGCACATT; ACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGA; GTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTT; GGGGGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACA; ACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGG; TGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG; GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGC; CGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGT; GGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAG; CAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCC; CTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTC; CTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA; CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTG; CTTCAGAAACACTTGACTGCTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219942 protein sequence; Red=Cloning site Green=Tags(s); ; MKNSRTWAWRAPVELFLLCAALGCLSLPGSRGERPHSFGSNAVNKSFAKSRQMRSVDVTLMPIDCELSSW; SSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRCEGFVCAQTGRCVNRRLLC; NGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLDHRYYAGGCSPHYILNTRF; RKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIR; RTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVL; GGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMV; EDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMK; QALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGR; RKTRQRQCNNPPPQNGGSPCSGPASETLDCS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000066
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000066.3
• RefSeq Size: 2178 bp
• RefSeq ORF: 1776 bp
• Locus ID: 732
• UniProt ID: P07358
• Cytogenetics: 1p32.2
• Domains: ldl_recept_a, MACPF, tsp_1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 67 kDa
• Summary: This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]