OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Complement factor 8 beta (C8B) (NM_000066) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC219942
C8B (Myc-DDK-tagged)-Human complement component 8, beta polypeptide (C8B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $550.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA334645 Rabbit Polyclonal Anti-C8B Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Complement factor 8 beta or NM_000066"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Complement factor 8 beta
Synonyms C82
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219942 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCT
GTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTT
TGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGG
TCCTCTTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTC
AGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAG
AAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC
AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGC
ATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGA
GGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTT
AGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGT
ATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGG
TTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG
AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAAGTAGCACATT
ACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGA
GTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTT
GGGGGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACA
ACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGG
TGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG
GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGC
CGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGT
GGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAG
CAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCC
CTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTC
CTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA
CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTG
CTTCAGAAACACTTGACTGCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219942 protein sequence
Red=Cloning site Green=Tags(s)

MKNSRTWAWRAPVELFLLCAALGCLSLPGSRGERPHSFGSNAVNKSFAKSRQMRSVDVTLMPIDCELSSW
SSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRCEGFVCAQTGRCVNRRLLC
NGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLDHRYYAGGCSPHYILNTRF
RKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIR
RTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVL
GGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMV
EDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMK
QALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGR
RKTRQRQCNNPPPQNGGSPCSGPASETLDCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000066
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000066.3
RefSeq Size 2178 bp
RefSeq ORF 1776 bp
Locus ID 732
UniProt ID P07358
Cytogenetics 1p32.2
Domains ldl_recept_a, MACPF, tsp_1
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
MW 67 kDa
Summary This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2013
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_000066" in other vectors (4)
SKU Description Size Price
RC219942L3 Lenti ORF clone of Human complement component 8, beta polypeptide (C8B), Myc-DDK-tagged 10 ug
$850.00
RC219942L4 Lenti ORF clone of Human complement component 8, beta polypeptide (C8B), mGFP tagged 10 ug
$850.00
RG219942 C8B (tGFP-tagged) - Human complement component 8, beta polypeptide (C8B) 10 ug
$489.00 $750.00
SC115997 C8B (untagged)-Human complement component 8, beta polypeptide (C8B) 10 ug
$551.00

Plasmid Map for RC219942

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219942
Circular map for RC219942

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products