Complement factor 8 beta (C8B) (NM_000066) Human Untagged Clone

SKU SC115997

C8B (untagged)-Human complement component 8, beta polypeptide (C8B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Complement factor 8 beta
• Synonyms: C82
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115997 sequence for NM_000066 edited (data generated by NextGen Sequencing); ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCT; GCCCTGGGCTGTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCA; AATGCAGTCAACAAGAGCTTTGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTG; ATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTCTTGGACCACATGTGACCCCTGTCAG; AAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGC; AACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAGAAGTCAAGTG; CGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC; AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAA; AAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAAT; TTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGC; TCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACG; CCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTT; GAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATA; CCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG; AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAA; GTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGA; GTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTT; GGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATG; AACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAA; AATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTA; GGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG; GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATAC; CAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCA; GCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCC; TATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTT; AGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGC; TGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAG; AATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA; CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGT; TCAGGCCCTGCTTCAGAAACACTTGACTGCTCCTAG; ; Clone variation with respect to NM_000066.2; 349 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000066 unedited; NNNNNGGGTTCAGATTTGTATACGACTCCTATAGGCGGCCGCGGTTTACTTTGCAGAGCT; ATTTGCTCTTGGGGACAGAAGCTGACAGTGGCACTCACAGCACAGGCTTGTTATGGGTCT; AGCAGCCTCTGTGGCATCTCCTGTCACATTGGGAAAATGAAGAATTCCAGGACATGGGCT; TGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCTGTCTCAGTTTGCCT; GGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTTTGCT; AAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCT; AGTTGGTCCTCTTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTAC; TTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAA; GACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTNTGTGTGTGCA; CAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGNCATGGGGACAATGACTGTGGAGAC; CAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAA; TACTGNGGAAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTACAAACAGTTTTGAGGGC; CCCAGTCTTGATCACAGGTATTATNGCAGGNTGGATGCTCCCCGCATTACATNCTGACAA; CGAGGNTTTANGAAGCCCTACNNATGTGAAAGCTACACGCCACAGACCCCAGGCAATACG; AATTCTATTAAAAGAGTNTGAATCTACTCAGATTTGAACGCATGTCCANAGAATGGCAGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000066 unedited; NTTTTTGACTTGNACNCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTTT; TAAGCCTTTAACTCAGTATTTATTTAAATCAACTTGCATTTTACAAGGTAACATCTTTAT; TTTAAACAGCTTGCATGGCACTGCCTTTTGCCCTTGCATGAACTCCAGGTGGAAACTGGT; GTAGGGCTGAGCTGGCATGAGTTCTTGAGGGCTCAGGGCTCTCATTGTATGTAGCCCACT; GCTGTATCATCTGCTAGGAGCAGTCAAGTGTTTCTGAAGCAGGGCCTGAACAGGGGCTAC; CCCCATTTTGAGGAGGTGGATTGTTACACTGCCTTTGTCTTGTCTTACGTCTTCCAGAGC; ATGAAGACCAATTTGACCAGCAATTCCACTTCCCATCAATGGGGGTATTCTTCCGATAGG; AGACCTCACAGGCTAGGCCTTGGGATCCAACAGGACAGATGCAGTCACAGCGTGATCCTT; TCAGGACAGGGACTCCATTTCCTTGGCAGGGAGCACAGTGGCAGGAACTAACTTCCTTCT; GGAACTCCTCCAGTGCCTGCTTCATGTTCTGCCTCACTGTGCTGGAATAGGCAAAATCTG; TGGCTGTCACTAGTTCATACAGAGGCTCCACCTTAACTTTGATGATGGCTGGGGTTGTAC; TGCACAGCGTCTCCCCACTCCTGCATCAGGTCCGCCGTCGGCAGCTCCTGGTATGCCAGG; GGTGGTGATGTGCTCACTTGCCCCTCCTCGTACAGGACCACCAAGTCCTCCCCATGGNGN; NCCTCNTGNTTCTGGCTTTTATTTCATTCAGAATACCTCTGCATTTGCCTACAGACACCC; CAGACTGACGTAGACCTCTTCATGGCACCACCCATTTAAAANTCATTTTGGCACNNNGCA; TGAACGTGTAAAAGNATATCTCCTCTCTCATGCCCCTCTTGTATAACCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000066
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000066.2, NP_000057.1
• RefSeq Size: 2053 bp
• RefSeq ORF: 1776 bp
• Locus ID: 732
• UniProt ID: P07358
• Cytogenetics: 1p32.2
• Domains: ldl_recept_a, MACPF, tsp_1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Summary: This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]; Transcript Variant: This variant (1) encodes the longest isoform (1).