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NIRF (UHRF2) (NM_152896) Human Tagged ORF Clone

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SKU
RC219579
UHRF2 (Myc-DDK-tagged)-Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $734.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NIRF
Synonyms NIRF; RNF107; TDRD23; URF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219579 representing NM_152896
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGATACAGGTTCGCACCATTGATGGCTCCAAGACGTGCACCATTGAGGACGTGTCTCGCAAAGCCA
CGATTGAGGAGCTGCGCGAGCGGGTGTGGGCGCTGTTCGACGTGCGGCCCGAATGCCAGCGCCTCTTCTA
CCGGGGCAAGCAGTTGGAAAATGGATATACCTTATTTGATTATGATGTTGGACTGAATGATATAATTCAG
CTGCTAGTTCGCCCAGACCCTGATCATCTTCCTGGCACATCTACACAGATTGAGGCTAAACCCTGTTCTA
ATAGTCCACCTAAAGTAAAGAAAGCTCCGAGGGTAGGACCTTCCAATCAGCCATCTACATCAGCTCGTGC
CCGTCTTATTGATCCTGGCTTTGGAATATATAAGGTAAATGAATTGGTGGATGCCAGAGATGTCGGCCTT
GGTGCTTGGTTTGAAGCACACATACATAGTGTTACTAGAGCTTCTGATGGACAGTCACGTGGCAAAACTC
CACTGAAGAATGGCAGTTCTTGTAAAAGGACTAATGGAAATATAAAGCATAAATCCAAAGAGAACACAAA
TAAATTGGACAGTGTACCCTCTACGTCTAATTCAGACTGTGTTGCTGCTGATGAAGACGTTATTTACCAT
ATCCAGTATGATGAATACCCAGAAAGCGGTACTCTAGAAATGAATGTCAAGGATCTTAGACCACGAGCTA
GAACCATTTTGAAATGGAATGAACTAAATGTTGGTGATGTGGTAATGGTTAATTATAATGTAGAAAGTCC
TGGACAAAGAGGATTCTGGTTTGATGCAGAAATTACCACATTGAAGACAATCTCAAGGACCAAAAAAGAA
CTTCGTGTGAAAATTTTCCTGGGGGGTTCTGAAGGAACATTAAATGACTGCAAGATAATATCTGTAGATG
AAATCTTCAAGATTGAGAGACCTGGAGCCCATCCCCTTTCATTTGCAGATGGAAAGTTTTTAAGGCGAAA
TGACCCTGAATGTGACCTGTGTGGTGGAGACCCAGAAAAGAAATGTCATTCTTGCTCCTGTCGTGTATGT
GGTGGGAAACATGAACCCAACATGCAGCTTCTGTGTGATGAATGTAATGTGGCTTATCATATTTACTGTC
TGAATCCACCTTTGGATAAAGTCCCAGAAGAGGAATACTGGTATTGTCCTTCTTGTAAAACTGATTCCAG
TGAAGTTGTAAAGGCTGGTGAAAGACTCAAGATGAGTAAAAAGAAAGCAAAGATGCCGTCAGCTAGTACT
GAAAGCCGAAGAGACTGGGGCAGGGGAATGGCTTGTGTTGGTCGTACGAGAGAATGTACTATTGTCCCTT
CTAATCATTATGGACCCATTCCTGGTATTCCTGTTGGATCAACTTGGAGATTTAGAGTTCAGGTGAGCGA
AGCAGGTGTTCACAGACCCCATGTTGGTGGAATTCATGGTCGAAGTAATGATGGGGCTTATTCTCTTGTA
CTGGCTGGTGGATTTGCGGATGAAGTCGACCGAGGTGATGAGTTCACATACACTGGAAGCGGTGGTAAAA
ATCTTGCTGGTAACAAAAGAATTGGTGCACCTTCAGCTGATCAAACATTAACAAACATGAACAGGGCATT
GGCCCTAAACTGTGATGCTCCATTGGATGATAAAATTGGAGCAGAGTCTCGGAATTGGAGAGCTGGTAAG
CCAGTCAGAGTGATACGCAGTTTTAAAGGGAGGAAGATCAGCAAATATGCTCCTGAAGAAGGCAACAGAT
ATGATGGCATTTATAAGGTGGTGAAATACTGGCCAGAGATTTCATCAAGCCATGGATTCTTGGTTTGGCG
CTATCTTTTAAGAAGAGATGATGTTGAACCTGCTCCTTGGACCTCTGAAGGAATAGAACGGTCAAGGAGA
TTATGTCTACGTTTACAGTATCCAGCAGGTTACCCTTCAGATAAAGAAGGGAAGAAGCCTAAAGGACAGT
CAAAGAAGCAGCCCAGTGGAACCACAAAAAGGCCAATTTCAGATGATGACTGTCCAAGTGCCTCCAAAGT
GTACAAAGCATCAGATTCAGCAGAAGCAATTGAGGCTTTTCAACTAACTCCTCAACAGCAACATCTCATC
AGAGAAGATTGTCAAAACCAGAAGCTGTGGGATGAAGTGCTTTCACATCTTGTGGAAGGACCAAATTTTC
TGAAAAAATTGGAACAATCTTTTATGTGCGTTTGCTGTCAGGAGCTAGTTTACCAGCCTGTGACAACTGA
GTGCTTCCACAATGTCTGTAAAGATTGTCTACAGCGCTCCTTTAAGGCACAGGTTTTCTCCTGCCCTGCT
TGCCGGCATGATCTTGGCCAGAATTACATCATGATTCCCAATGAGATTCTGCAGACTCTACTTGACCTTT
TCTTCCCTGGCTACAGCAAAGGACGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219579 representing NM_152896
Red=Cloning site Green=Tags(s)

MWIQVRTIDGSKTCTIEDVSRKATIEELRERVWALFDVRPECQRLFYRGKQLENGYTLFDYDVGLNDIIQ
LLVRPDPDHLPGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGL
GAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIKHKSKENTNKLDSVPSTSNSDCVAADEDVIYH
IQYDEYPESGTLEMNVKDLRPRARTILKWNELNVGDVVMVNYNVESPGQRGFWFDAEITTLKTISRTKKE
LRVKIFLGGSEGTLNDCKIISVDEIFKIERPGAHPLSFADGKFLRRNDPECDLCGGDPEKKCHSCSCRVC
GGKHEPNMQLLCDECNVAYHIYCLNPPLDKVPEEEYWYCPSCKTDSSEVVKAGERLKMSKKKAKMPSAST
ESRRDWGRGMACVGRTRECTIVPSNHYGPIPGIPVGSTWRFRVQVSEAGVHRPHVGGIHGRSNDGAYSLV
LAGGFADEVDRGDEFTYTGSGGKNLAGNKRIGAPSADQTLTNMNRALALNCDAPLDDKIGAESRNWRAGK
PVRVIRSFKGRKISKYAPEEGNRYDGIYKVVKYWPEISSSHGFLVWRYLLRRDDVEPAPWTSEGIERSRR
LCLRLQYPAGYPSDKEGKKPKGQSKKQPSGTTKRPISDDDCPSASKVYKASDSAEAIEAFQLTPQQQHLI
REDCQNQKLWDEVLSHLVEGPNFLKKLEQSFMCVCCQELVYQPVTTECFHNVCKDCLQRSFKAQVFSCPA
CRHDLGQNYIMIPNEILQTLLDLFFPGYSKGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_152896
ORF Size 2406 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152896.3
RefSeq Size 3621 bp
RefSeq ORF 2409 bp
Locus ID 115426
UniProt ID Q96PU4
Cytogenetics 9p24.1
Protein Families Druggable Genome, Transcription Factors
MW 89.8 kDa
Summary This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. provided by RefSeq, Feb 2012
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"NM_152896" in other vectors (6)
SKU Description Size Price
RC219579L1 Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), Myc-DDK-tagged 10 ug
$1,034.00
RC219579L2 Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mGFP tagged 10 ug
$1,034.00
RC219579L3 Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), Myc-DDK-tagged 10 ug
$1,034.00
RC219579L4 Lenti ORF clone of Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2), mGFP tagged 10 ug
$1,034.00
RG219579 UHRF2 (tGFP-tagged) - Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2) 10 ug
$789.00 $934.00
SC124707 UHRF2 (untagged)-Human ubiquitin-like with PHD and ring finger domains 2 (UHRF2) 10 ug
$735.00

file-alt Citations

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