OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

DUSP18 (NM_152511) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC219496
DUSP18 (Myc-DDK-tagged)-Human dual specificity phosphatase 18 (DUSP18)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "DUSP18 or NM_152511"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUSP18
Synonyms DSP18; DUSP20; LMWDSP20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219496 representing NM_152511
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCACCCTCGTGTGCCTTCCCAGTTCAGTTCCGGCAGCCCTCAGTCAGCGGCCTCTCGCAGATAA
CCAAAAGCCTGTATATCAGCAATGGTGTGGCCGCCAACAACAAGCTCATGCTGTCTAGCAACCAGATCAC
CATGGTCATCAATGTCTCAGTGGAGGTAGTGAACACCTTGTATGAGGATATCCAGTACATGCAGGTACCT
GTGGCTGACTCCCCTAACTCACGTCTCTGTGACTTCTTTGACCCTATTGCTGACCATATCCACAGCGTGG
AGATGAAGCAGGGCCGTACTTTGCTGCACTGTGCTGCTGGTGTGAGCCGCTCAGCTGCCCTGTGCCTCGC
CTACCTCATGAAGTACCACGCCATGTCCCTGCTGGACGCCCACACGTGGACCAAGTCATGCCGGCCCATC
ATCCGACCCAACAGCGGCTTTTGGGAGCAGCTCATCCACTATGAGTTCCAATTGTTTGGCAAGAACACTG
TGCACATGGTCAGTTCCCCAGTGGGAATGATCCCTGACATCTATGAGAAGGAAGTCCGTTTGATGATTCC
ACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219496 representing NM_152511
Red=Cloning site Green=Tags(s)

MTAPSCAFPVQFRQPSVSGLSQITKSLYISNGVAANNKLMLSSNQITMVINVSVEVVNTLYEDIQYMQVP
VADSPNSRLCDFFDPIADHIHSVEMKQGRTLLHCAAGVSRSAALCLAYLMKYHAMSLLDAHTWTKSCRPI
IRPNSGFWEQLIHYEFQLFGKNTVHMVSSPVGMIPDIYEKEVRLMIPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152511
ORF Size 564 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152511.5
RefSeq Size 2453 bp
RefSeq ORF 567 bp
Locus ID 150290
UniProt ID Q8NEJ0, A0A024R1L2
Cytogenetics 22q12.2
Domains DSPc
Protein Families Druggable Genome, Phosphatase
MW 20.9 kDa
Summary Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP18 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 PubMed 19228121).supplied by OMIM, Dec 2009
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_152511" in other vectors (4)
SKU Description Size Price
RC219496L3 Lenti ORF clone of Human dual specificity phosphatase 18 (DUSP18), Myc-DDK-tagged 10 ug
$600.00
RC219496L4 Lenti ORF clone of Human dual specificity phosphatase 18 (DUSP18), mGFP tagged 10 ug
$600.00
RG219496 DUSP18 (tGFP-tagged) - Human dual specificity phosphatase 18 (DUSP18) 10 ug
$489.00 $500.00
SC101321 DUSP18 (untagged)-Human dual specificity phosphatase 18 (DUSP18) 10 ug
$300.00

Plasmid Map for RC219496

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219496
Circular map for RC219496

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products